We are the change we seek

Lindsey, Merry L.; Kassiri, Zamaneh; Keehan, Kara Hansell; Brunt, Keith R.; Carter, Jason R.; Kirk, Jonathan A.; Kleinbongard, Petra; LeBlanc, Amanda J.; Ripplinger, Crystal M.

doi:10.1152/ajpheart.00090.2021

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…Likewise, in some cases the ex vivo innervated heart preparations did not capture at a particular pacing frequency, which impacted sample size for some ratematched parameters. N are specified in each figure legend and data from both sexes pooled, with O and X symbols indicating female and male sex, respectively (48,49). Statistical analysis was performed using GraphPad Prism 9.…”

Section: Statisticsmentioning

confidence: 99%

Chronic nicotine exposure is associated with electrophysiological and sympathetic remodeling in the intact rabbit heart

Guevara,

Smith,

Caldwell

et al. 2023

Preprint

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Nicotine is the primary addictive component in tobacco products. Through its actions on the heart and autonomic nervous system, nicotine exposure is associated with electrophysiological changes and increased arrhythmia susceptibility. However, the underlying mechanisms are unclear. To address this, we treated rabbits with transdermal nicotine (NIC, 21 mg/day) or control (CT) patches for 28 days prior to performing dual optical mapping of transmembrane potential (RH237) and intracellular Ca2+(Rhod-2 AM) in isolated hearts with intact sympathetic innervation. Sympathetic nerve stimulation (SNS) was performed at the 1st– 3rdthoracic vertebrae, and β-adrenergic responsiveness was additionally evaluated as changes in heart rate (HR) following norepinephrine (NE) perfusion. Baselineex vivoHR and SNS stimulation threshold were increased in NIC vs. CT (P= 0.004 andP= 0.003 respectively). Action potential duration alternans emerged at longer pacing cycle lengths (PCL) in NIC vs. CT at baseline (P= 0.002) and during SNS (P= 0.0003), with similar results obtained for Ca2+transient alternans. SNS reduced the PCL at which alternans emerged in CT but not NIC hearts. NIC exposed hearts also tended to have slower and reduced HR responses to NE perfusion. While fibrosis was unaltered, NIC hearts had lower sympathetic nerve density (P= 0.03) but no difference in NE content vs. CT. These results suggest both sympathetic hypo-innervation of the myocardium and diminished β-adrenergic responsiveness with NIC. This autonomic remodeling may underlie the increased risk of arrhythmias associated with nicotine exposure, which may be further exacerbated with continued long-term usage.NEW & NOTEWORTHYHere we show that chronic nicotine exposure was associated with increased heart rate, lower threshold for alternans and reduced sympathetic electrophysiological responses in the intact rabbit heart. We suggest that this was due to the sympathetic hypo-innervation of the myocardium and diminished β- adrenergic responsiveness observed following nicotine treatment. Though these differences did not result in increased arrhythmia propensity in our study, we hypothesize that prolonged nicotine exposure may exacerbate this pro-arrhythmic remodeling.

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Section: Statisticsmentioning

confidence: 99%

Chronic nicotine exposure is associated with electrophysiological and sympathetic remodeling in the intact rabbit heart

Guevara,

Smith,

Caldwell

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The launch of both of these initiatives constitutes part of a broader strategy in place at the journals of the American Physiological Society to promote our early career members, to maintain an exemplary standard of peer review that is supported by an integrated succession plan, and to recruit and engage a diverse and inclusive pool of editors, authors, and peer reviewers (2)(3)(4)(5)(6)(7). As Editors at AJP-Lung, we are excited to work with our early career members to continue to champion these principles and advance the field of respiratory physiology.…”

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confidence: 99%