WD Repeat-containing Protein 5 (WDR5) Localizes to the Midbody and Regulates Abscission

Bailey, Jeffrey K; Fields, Alexander T.; Cheng, Kaijian; Lee, Albert; Wagenaar, Eric; Lagrois, Remy; Schmidt, Bailey; Xia, Bin; Ma, Dzwokai

doi:10.1074/jbc.m114.623611

Cited by 31 publications

(26 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Wdr5 Moonlights Off Chromatinmentioning

confidence: 99%

“…WDR5 localizes to the mitotic spindle and to the midbody in dividing human cells [ 80 , 81 ]. This localization depends on the integrity of the Win site, as mutations in this site of WDR5 prevent its stable association with the midbody [ 80 ]. Every indication is that binding of WDR5 to the midbody and spindle is functionally relevant, as these WDR5 Win site mutants also fail to rescue mitotic defects associated with WDR5 knock-down [ 82 ].…”

Section: Wdr5 Moonlights Off Chromatinmentioning

confidence: 99%

See 1 more Smart Citation

Moonlighting with WDR5: A Cellular Multitasker

Guarnaccia

Tansey

2018

JCM

113

218

View full text Add to dashboard Cite

WDR5 is a highly conserved WD40 repeat-containing protein that is essential for proper regulation of multiple cellular processes. WDR5 is best characterized as a core scaffolding component of histone methyltransferase complexes, but emerging evidence demonstrates that it does much more, ranging from expanded functions in the nucleus through to controlling the integrity of cell division. The purpose of this review is to describe the current molecular understandings of WDR5, discuss how it participates in diverse cellular processes, and highlight drug discovery efforts around WDR5 that may form the basis of new anti-cancer therapies.

show abstract

Section: Wdr5 Moonlights Off Chromatinmentioning

confidence: 99%

Section: Wdr5 Moonlights Off Chromatinmentioning

confidence: 99%

Moonlighting with WDR5: A Cellular Multitasker

Guarnaccia

Tansey

2018

JCM

113

218

View full text Add to dashboard Cite

show abstract

“…In addition to JADE1S, several transcription factors, histone and DNA modifiers with predominantly nuclear localization such as Brd4 and WDR5, have been found to regulate cytokinesis and abscission 44, 45 . Recently, it has been reported that the novel Abscission/NoCut Checkpoint Regulator (ANCHR) protein functions in Aurora-B-dependent abscission checkpoint control in HeLa cells 46 .…”

Section: Cellular and Biochemical Functionmentioning

confidence: 99%

Structure, function and regulation of jade family PHD finger 1 (JADE1)

Panchenko

2016

Gene

View full text Add to dashboard Cite

The family of JADE proteins includes three paralogues encoded by individual genes and designated PHF17 (JADE1), PHF16 (JADE2), and PHF15 (JADE3). All three JADE proteins bear in tandem two Plant Homeo-domains (PHD) which are zinc finger domains. This review focuses on one member of the JADE family, JADE1. Studies addressing the biochemical, cellular and biological role of JADE1 are discussed. Recent discoveries of JADE1 function in the regulation of the epithelial cell cycle with potential relevance to disease are presented. Unresolved questions and future directions are formulated.

show abstract

“…Recently, more and more epigenetic regulating proteins, which mainly localize in the nucleus in interphase, have been reported to obtain new localizations and functions during mitosis. Some typical examples are that HDAC3 localizes on the spindles and affects spindle assembly (38); MeCP2 co-localizes with centrosomes and spindles, and its loss disturbs spindle morphology and mitosis (39); and WDR5 shows localization on the midbody during cytokinesis and regulates this process (40). In our study, immunostaining showed that JMJD5 also changed its localization during mitosis.…”

Section: Discussionmentioning

confidence: 72%

JMJD5 (Jumonji Domain-containing 5) Associates with Spindle Microtubules and Is Required for Proper Mitosis

et al. 2016

Journal of Biological Chemistry

View full text Add to dashboard Cite

Precise mitotic spindle assembly is a guarantee of proper chromosome segregation during mitosis. Chromosome instability caused by disturbed mitosis is one of the major features of various types of cancer. JMJD5 has been reported to be involved in epigenetic regulation of gene expression in the nucleus, but little is known about its function in mitotic process. Here we report the unexpected localization and function of JMJD5 in mitotic progression. JMJD5 partially accumulates on mitotic spindles during mitosis, and depletion of JMJD5 results in significant mitotic arrest, spindle assembly defects, and sustained activation of the spindle assembly checkpoint (SAC). Inactivating SAC can efficiently reverse the mitotic arrest caused by JMJD5 depletion. Moreover, JMJD5 is found to interact with tubulin proteins and associate with microtubules during mitosis. JMJD5-depleted cells show a significant reduction of ␣-tubulin acetylation level on mitotic spindles and fail to generate enough interkinetochore tension to satisfy the SAC. Further, JMJD5 depletion also increases the susceptibility of HeLa cells to the antimicrotubule agent. Taken together, these results suggest that JMJD5 plays an important role in regulating mitotic progression, probably by modulating the stability of spindle microtubules.

show abstract

WD Repeat-containing Protein 5 (WDR5) Localizes to the Midbody and Regulates Abscission

Cited by 31 publications

References 82 publications

Moonlighting with WDR5: A Cellular Multitasker

Moonlighting with WDR5: A Cellular Multitasker

Structure, function and regulation of jade family PHD finger 1 (JADE1)

JMJD5 (Jumonji Domain-containing 5) Associates with Spindle Microtubules and Is Required for Proper Mitosis

Contact Info

Product

Resources

About