WBC27, an Adenosine Tri‐phosphate‐binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis

Dou, Xiaoying; Yang, Kezhen; Zeng, Yi; Wang, Wei; Chen, Li‐Qun; Zhang, Xue-Qin; Ye, De

doi:10.1111/j.1744-7909.2010.01010.x

Cited by 77 publications

(63 citation statements)

References 52 publications

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“…Many recent reports revealed the mechanism of biosynthesis and transport of pollen wall precursors in Arabidopsis and showed that the pollen wall is important for pollen development and protection (van der Meer et al, 1992;Matsuda et al, 1996;Aarts et al, 1997;Morant et al, 2007;de Azevedo Souza et al, 2009;Dobritsa et al, 2009Dobritsa et al, , 2010Quilichini et al, 2010;Xu et al, 2010;Choi et al, 2011;Dou et al, 2011). By contrast, studies on pollen coat lipids were mostly centered on pollen-stigma interactions, such as the self-incompatibility response and pollen hydration (Preuss et al, 1993;Piffanelli et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…In many organisms, including animals, insects, fungi, and plants, ABCG subfamily members are reported to be involved in lipid transport (Li and Prinz, 2004;Velamakanni et al, 2007;Kang et al, 2011), and the deletion of some members of the transporter family results in lipid-related diseases, growth defects, and sterility (Berge et al, 2000;Bird et al, 2007;Luo et al, 2007;Panikashvili et al, 2007;Ukitsu et al, 2007;Quilichini et al, 2010;Xu et al, 2010;Choi et al, 2011;Dou et al, 2011). Plants are highly enriched in a number of ABCG transporters, but only several of these have been studied to date.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Arabidopsis ABCG9 and ABCG31 ATP Binding Cassette Transporters in Pollen Fitness and the Deposition of Steryl Glycosides on the Pollen Coat

et al. 2014

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The pollen coat protects pollen grains from harmful environmental stresses such as drought and cold. Many compounds in the pollen coat are synthesized in the tapetum. However, the pathway by which they are transferred to the pollen surface remains obscure. We found that two Arabidopsis thaliana ATP binding cassette transporters, ABCG9 and ABCG31, were highly expressed in the tapetum and are involved in pollen coat deposition. Upon exposure to dry air, many abcg9 abcg31 pollen grains shriveled up and collapsed, and this phenotype was restored by complementation with ABCG9 pro :GFP:ABCG9. GFP-tagged ABCG9 or ABCG31 localized to the plasma membrane. Electron microscopy revealed that the mutant pollen coat resembled the immature coat of the wild type, which contained many electron-lucent structures. Steryl glycosides were reduced to about half of wild-type levels in the abcg9 abcg31 pollen, but no differences in free sterols or steryl esters were observed. A mutant deficient in steryl glycoside biosynthesis, ugt80A2 ugt80B1, exhibited a similar phenotype. Together, these results indicate that steryl glycosides are critical for pollen fitness, by supporting pollen coat maturation, and that ABCG9 and ABCG31 contribute to the accumulation of this sterol on the surface of pollen.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Role of Arabidopsis ABCG9 and ABCG31 ATP Binding Cassette Transporters in Pollen Fitness and the Deposition of Steryl Glycosides on the Pollen Coat

et al. 2014

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“…Some plant ABCG proteins have been reported to contribute to the synthesis of extracellular barriers. In Arabidopsis, ABCG11 (Cuticular Defect and Organ Fusion1/DESPERADO/White-Brown Complex11; Bird et al, 2007;Panikashvili et al, 2007Panikashvili et al, , 2010, ABCG12 (Eceriferum5/WBC12; Pighin et al, 2004), ABCG13 (Panikashvili et al, 2010), ABCG29 (Alejandro et al, 2012), ABCG32 (Bessire et al, 2011), and ABCG26 (WBC27; Xu et al, 2010;Quilichini et al, 2010Quilichini et al, , 2014Choi et al, 2011;Dou et al, 2011) have been shown to be involved in transport of lipidic compounds. Notably, it has been reported that these ABCG proteins have a broad substrates spectrum (for example, ABCG11 transports both cutin and wax monomers, and ABCG13 and ABCG32 mainly transport cutin monomers, whereas ABCG12 mainly transports wax precursors, and ABCG29 mainly transports monolignol).…”

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confidence: 99%

Two ATP Binding Cassette G (ABCG) Transporters, OsABCG26 and OsABCG15, Collaboratively Regulate Rice Male Reproduction

et al. 2015

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Male reproduction in higher plants requires the support of various metabolites, including lipid molecules produced in the innermost anther wall layer (the tapetum), but how the molecules are allocated among different anther tissues remains largely unknown. Previously, rice (Oryza sativa) ATP binding cassette G15 (ABCG15) and its Arabidopsis (Arabidopsis thaliana) ortholog were shown to be required for pollen exine formation. Here, we report the significant role of OsABCG26 in regulating the development of anther cuticle and pollen exine together with OsABCG15 in rice. Cytological and chemical analyses indicate that osabcg26 shows reduced transport of lipidic molecules from tapetal cells for anther cuticle development. Supportively, the localization of OsABCG26 is on the plasma membrane of the anther wall layers. By contrast, OsABCG15 is polarly localized in tapetal plasma membrane facing anther locules. osabcg26 osabcg15 double mutant displays an almost complete absence of anther cuticle and pollen exine, similar to that of osabcg15 single mutant. Taken together, we propose that OsABCG26 and OsABCG15 collaboratively regulate rice male reproduction: OsABCG26 is mainly responsible for the transport of lipidic molecules from tapetal cells to anther wall layers, whereas OsABCG15 mainly is responsible for the export of lipidic molecules from the tapetal cells to anther locules for pollen exine development.

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“…Sporopollenin precursors are synthesized mainly in tapetal cells and transported to the microspore surface by the involvement of many genes in which mutations often cause strong defects in exine development. These genes include: MALE STERILITY2 (MS2) (Aarts et al, 1997), FACELESS POLLEN1 (FLP1) (Ariizumi et al, 2003), NO EXINE FORMATION1 (NEF1) (Ariizumi et al, 2004), CYP703A2 (Morant et al, 2007), ACYL-CoA SYNTHETASE5 (ACOS5) (de Azevedo Souza et al, 2009), LESS ADHERENT POLLEN3 (LAP3), LAP5/POLYKETIDE SYNTHASE B (PKSB), LAP6/POLYKETIDE SYNTHASE A (PKSA) (Dobritsa et al, 2009a;Dobritsa et al, 2010;Kim et al, 2010), CYP704B1 (Dobritsa et al, 2009b), DIHYDROFLAVONOL 4-REDUCTASE-LIKE1 (DRL1)/ TETRAKETIDEa-PYRONE REDUCTASE2 (TKPR2) (Tang et al, 2009;Grienenberger et al, 2010), TKPR1 , and a member of ATPbinding cassette transporter gene ABCG26 (Quilichini et al, 2010;Choi et al, 2011;Dou et al, 2011;Kuromori et al, 2011). Although many of these genes are specifically expressed in tapetal cells, it has been proposed that microspores also synthesize and secrete sporopollenin at the initial stage of exine development in tetrads (Wallace et al, 2011).…”

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confidence: 99%

KNS4/UPEX1: A Type II Arabinogalactan β-(1,3)-Galactosyltransferase Required for Pollen Exine Development

et al. 2016

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Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we named kaonashi (kns). Here we describe the identification of the causal gene of kns4 that was found to be a member of the CAZy glycosyltransferase 31 gene family, identical to UNEVEN PATTERN OF EXINE1, and the biochemical characterization of the encoded protein. The characteristic exine phenotype in the kns4 mutant is related to an abnormality of the primexine matrix laid on the surface of developing microspores. Using light microscopy with a combination of type II arabinogalactan (AG) antibodies and staining with the arabinogalactan-protein (AGP)-specific b-Glc Yariv reagent, we show that the levels of AGPs in the kns4 microspore primexine are considerably diminished, and their location differs from that of wild type, as does the distribution of pectin labeling. Furthermore, kns4 mutants exhibit reduced fertility as indicated by shorter fruit lengths and lower seed set compared to the wild type, confirming that KNS4 is critical for pollen viability and development. KNS4 was heterologously expressed in Nicotiana benthamiana, and was shown to possess b-(1,3)-galactosyltransferase activity responsible for the synthesis of AG glycans that are present on both AGPs and/or the pectic polysaccharide rhamnogalacturonan I. These data demonstrate that defects in AGP/pectic glycans, caused by disruption of KNS4 function, impact pollen development and viability in Arabidopsis.Pollen, the male gametophyte of all seed plants, is crucial for reproductive success. Due to the harsh environmental conditions that pollen must survive in, it has developed an elaborate and specialized cell wall. However, despite its importance our knowledge of the fine structure and assembly of the pollen grain wall is poorly understood (Newbigin et al., 2009;Ariizumi and Toriyama, 2011;Quilichini et al., 2015;Shi et al., 2015). Arabidopsis (Arabidopsis thaliana) provides an ideal genetic and cell biological model to dissect the assembly of the components of the pollen grain wall. Arabidopsis pollen grains have a typical surface structure that consists of an inner intine layer, which is usually made up of cellulose and pectin, and the outer exine, which is largely composed of sporopollenin (Li et al., 1995). The exine is further subdivided into inner nexine and outer sexine. The sexine has a three-dimensional (3D) structure composed of many columns called baculae and a roof called tectum. These two structures give the Arabidopsis pollen surface a reticulate appearance with a uniform mesh size. The nexine is composed of outer nexine I (foot layer), which contains sporopollenin, and inner nexine II (endexine; Ariizumi and Toriyama, 2011;Jiang et al., 2013). A recent study suggests that arabinogalactan proteins (AGPs) are key constituents of nexine with express...

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WBC27, an Adenosine Tri‐phosphate‐binding Cassette Protein, Controls Pollen Wall Formation and Patterning in Arabidopsis

Cited by 77 publications

References 52 publications

The Role of Arabidopsis ABCG9 and ABCG31 ATP Binding Cassette Transporters in Pollen Fitness and the Deposition of Steryl Glycosides on the Pollen Coat

The Role of Arabidopsis ABCG9 and ABCG31 ATP Binding Cassette Transporters in Pollen Fitness and the Deposition of Steryl Glycosides on the Pollen Coat

Two ATP Binding Cassette G (ABCG) Transporters, OsABCG26 and OsABCG15, Collaboratively Regulate Rice Male Reproduction

KNS4/UPEX1: A Type II Arabinogalactan β-(1,3)-Galactosyltransferase Required for Pollen Exine Development

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