Waxy Gene-Orthologs in Wheat × Thinopyrum Amphidiploids

Abstract

<jats:p>Starch, as the main component of grain in cereals, serves as the major source of calories in staple food and as a raw material for industry. As the technological and digestive properties of starch depend on its content, the management of its components, amylose and amylopectin, is of great importance. The starch properties of wheat grain can be attuned using allelic variations of genes, including granule-bound starch synthase I (GBSS I), or Wx. The tertiary gene pool, including wheatgrass (Thinopyrum) species, provides a wide spectrum of genes-orthologs that can be used to increase the allelic diversity of wheat genes by wide hybridization. Octaploid partial wheat–wheatgrass hybrids (amphidiploids, WWGHs) combine the complete genome of bread wheat (BBAADD), and a mixed genome from the chromosomes of intermediate wheatgrass (Thinopyrum intermedium, genomic composition JrJrJvsJvsStSt) and tall wheatgrass (Th. ponticum, JJJJJJJsJsJsJs). Thus, WWGHs may carry Wx genes not only of wheat (Wx-B1, Wx-A1 and Wx-D1) but also of wheatgrass origin. We aimed to assess the level of amylose in starch and investigate the polymorphism of Wx genes in 12 accessions of WWGHs. Additionally, we characterized orthologous Wx genes in the genomes of wild wheat-related species involved in the development of the studied WWGHs, Th. intermedium and Th. ponticum, as well as in the putative donors of their subgenomes, bessarabian wheatgrass (Th. bessarabicum, JbJb) and bluebunch wheatgrass (Pseudoroegneria stipifolia, St1St1St2St2). Although no significant differences in amylose content were found between different WWGH accessions, SDS-PAGE demonstrated that at least two WWGHs have an additional band. We sequenced the Wx gene-orthologs in Th. bessarabicum, P. stipifolia, Th. intermedium and Th. ponticum, and developed a WXTH marker that can discriminate the Thinopyrum Wx gene in the wheat background, and localized it to the 7E chromosome in Th. elongatum. Using the WXTH marker we revealed the allelic polymorphism of the Thinopyrum Wx gene in the studied WWGHs. The applicability of Thinopyrum Wx genes in wheat breeding and their effect on starch quality are discussed.</jats:p>

Trends and Perspectives for the Use of Crop Wild Relatives in Crop Breeding

“…In addition to disease resistance, other agronomic traits such as male sterility (Sigareva and Earle, 1997 in cabbage) and abiotic stress tolerance (Wei et al, 2015 in tobacco) have also been transferred by wide hybridization. Successful development and characterization of amphidiploids have been reported in several crops such as wheat Song et al, 2019;Klimushina et al, 2020;Zuo et al, 2020;, rice (Wang et al, 2013;), peanut (de Paula et al, 2017Li-Na et al, 2017;Favero et al, 2020;Balleń-Taborda et al, 2022), Brassica (Thomsen, 1990;Song et al, 1993), Vigna (Mjnocha et al, 1991), Dianthus (Nimura et al, 2006), Cucumis (Chen et al, 2002), and Solanum (Khan et al, 2013;. Even though many workers have attempted and succeeded in developing interspecific hybrids, the introgressed lines could be utilized only after eliminating the fertility barriers associated with the hybrids.…”

Genetic enhancement of okra [Abelmoschus esculentus (L.) Moench] germplasm through wide hybridization

“…In addition to disease resistance, other agronomic traits such as male sterility (Sigareva and Earle, 1997 in cabbage) and abiotic stress tolerance (Wei et al, 2015 in tobacco) have also been transferred by wide hybridization. Successful development and characterization of amphidiploids have been reported in several crops such as wheat (Zhang et al, 2016;Song et al, 2019;Klimushina et al, 2020;Zuo et al, 2020;Ren et al, 2022), rice (Wang et al, 2013;Kumar et al, 2019), peanut (de Paula et al, 2017;Li-Na et al, 2017;Favero et al, 2020;Balleń-Taborda et al, 2022), Brassica (Thomsen, 1990;Song et al, 1993), Vigna (Mjnocha et al, 1991), Dianthus (Nimura et al, 2006), Cucumis (Chen et al, 2002), and Solanum (Khan et al, 2013;Zhou et al, 2018). Even though many workers have attempted and succeeded in developing interspecific hybrids, the introgressed lines could be utilized only after eliminating the fertility barriers associated with the hybrids.…”

Androgenic response of Triticum durum-Dasypyrum villosum amphidiploids and their parental forms

“…Although contemporary science has reached high levels of use of the latter two technologies, wide hybridization remains a main conventional tool for achieving high genetic variabi lity. There is a large amount of research on amphidiploids de veloped through wide hybridization among the cereal crops (Zhang et al, 2010;Ming et al, 2011;Babaiants et al, 2012;Stoyanov, 2013Stoyanov, , 2014Dai et al, 2015;Nemeth et al, 2015;Song et al, 2019;Klimushina et al, 2020;Zuo et al, 2020;Kiani et al, 2021). One of the most promising species for enrichment of the genome of common and durum wheat, however, is Dasypyrum villosum.…”