Waxy and Soluble Starch SynthaseII-3 Alleles Regulating Rice Resistant Starch Contents from Different Processing Status

Abstract

BackgroundResistant Starch (RS) is a healthy dietary fiber that has functions of regulating diabetes, hypertension and obesity. Previous studies mainly focused on investigating RS in raw rice or cooked rice separately, which may receive different results. ResultsIn this study, ninety-nine lines from a recombinant inbred line (RIL) were selected to investigate the effects of starch synthesis-related genes on the RS content in different process status. RS content in rice will change by different processing ways. Waxy (Wx) played an important role in controlling RS content and Wxa could elevate RS content, and soluble starch synthaseII-3 (SSII-3) had an impact on RS2. Additionally, interaction of Wx and SSII-3 was responsible for variations of RS content in three sample types and RS2. Wx could affect RS in cooked rice and retrograded rice under the same SSII-3 allele. Moreover, the correlation analysis results indicated that RS was closely relative with many indexes of physicochemical properties. ConclusionsWx and SSII-3 could regulate RS content of rice, but SSII-3 especially affected RS2. The findings herein should provide useful information for molecular breeding of rice RS.