We aimed developing and experimentally validating methods for 3D scale‐selective polarimetry of multiply scattered fields in diffuse myocardium layers for mechanical myocardial injury prescription histological differential diagnostics. We used the synthesis of diffuse object field polarization‐interference registration and polarization‐inhomogeneous field digital holographic reconstruction and layer‐by‐layer complex amplitudes distributions The method for selection single and diffuse object field multiply scattered components polarization maps is proposed. The conditions for eliminating the distorting influence of a depolarized background high level are found. On the basis of еру object field single scattered component polarization maps a large‐scale selective wavelet analysis the criteria (markers) for mechanical myocardial injury different prescription diagnosis was determinate. Excellent accuracy mechanical injury myocardium necrotic changes with different duration using polarization‐interference wavelet differentiation were achieved.This article is protected by copyright. All rights reserved.