Wavelength shift in a whispering gallery microdisk due to bacterial sensing: A theoretical approach

Ghali, Hala; Bianucci, Pablo; Peter, Yves-Alain

doi:10.1016/j.sbsr.2017.01.004

Cited by 12 publications

(8 citation statements)

References 23 publications

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“…In the previous sections, an experimental wavelength shift was found for several bacterial concentrations. A theoretical expression that links the wavelength shift to the number of bacteria that bind to the surface of the microdisk was developed in [ 18 ] and shown in Equation ( 1 ). Using this expression, it was possible to obtain an approximate number of bacteria that bound to the surface of the microdisks and contributed to the reactive shift.…”

Section: Resultsmentioning

confidence: 99%

Real-Time Detection of Staphylococcus Aureus Using Whispering Gallery Mode Optical Microdisks

et al. 2016

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Whispering Gallery Mode (WGM) microresonators have recently been studied as a means to achieve real-time label-free detection of biological targets such as virus particles, specific DNA sequences, or proteins. Due to their high quality (Q) factors, WGM resonators can be highly sensitive. A biosensor also needs to be selective, requiring proper functionalization of its surface with the appropriate ligand that will attach the biomolecule of interest. In this paper, WGM microdisks are used as biosensors for detection of Staphylococcus aureus. The microdisks are functionalized with LysK, a phage protein specific for staphylococci at the genus level. A binding event on the surface shifts the resonance peak of the microdisk resonator towards longer wavelengths. This reactive shift can be used to estimate the surface density of bacteria that bind to the surface of the resonator. The limit of detection of a microdisk with a Q-factor around 104 is on the order of 5 pg/mL, corresponding to 20 cells. No binding of Escherichia coli to the resonators is seen, supporting the specificity of the functionalization scheme.

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Section: Resultsmentioning

confidence: 99%

Real-Time Detection of Staphylococcus Aureus Using Whispering Gallery Mode Optical Microdisks

et al. 2016

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“…used a phage-specific protein to capture Staphylococcus aureus onto a microdisk. 96 , 97 Gohring and Fan were able to detect and subtype human T cells. 98 WGM devices have been applied toward the detection of influenza A, M13, and the Bean pod mottle virus.…”

Section: Sensing Applicationsmentioning

confidence: 99%

Whispering-Gallery Sensors

Jiang

Qavi

Huang

et al. 2020

Matter

220

105

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Summary Optical whispering-gallery mode (WGM) microresonators, confining resonant photons in a microscale volume for long periods of time, strongly enhance light-matter interactions, making them an ideal platform for photonic sensors. One of the features of WGM sensors is their capability to respond to environmental perturbations that influence the optical mode distribution. The exceptional sensitivity of WGM devices, coupled with the diversity in their structures and the ease of integration with existing infrastructures, such as conventional chip-based technologies, has catalyzed the development of WGM sensors for a broad range of analytes. WGM sensors have been developed for multiplexed detection of clinically relevant biomolecules while also being adapted for the analysis of single-protein interactions. They have been used for the detection of materials in different phases and forms, including gases, liquids, and chemicals. Furthermore, WGM sensors have been used for a wide variety of field-based sensing applications, including electric field, magnetic field, force, pressure, and temperature. WGM sensors hold great potential for applications in life and environmental sciences. They are expected to meet the ever-increasing demand in sensor networks, the Internet of Things, and real-time health monitoring. Here we review the mechanisms, structures, parameters, and recent advances of WGM microsensors and discuss the future of this exciting research field.

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“…The remarkable sensitivity of these WGM optical resonances to environmental changes arises from their evanescent fields at the surface of a microsphere that extend to the interaction with the surrounding solution. Microsphere WGM sensors have been widely deployed as biosensors, enabling the detection of protein monolayers, biomolecular interactions, − bacteria, − and nanoparticles. − To push the boundary of detection sensitivity to its current highest single-molecule level to enable, for example, the detection of single atomic ions, plasmonic nanorods are strategically placed on the microspheres at the location of a WGM, as depicted in Figure a. These optoplasmonic WGM sensors capitalize on the evanescent field to excite plasmon resonance in plasmonic nanorods aligned with the electromagnetic field, leading to intensity enhancements at the nanorod tips; essentially placing the probing WGM field on the scale of the molecule, where only molecules interacting with the enhanced near-field region at the tips of the nanorods are detectable.…”

Section: Introductionmentioning

confidence: 99%

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

Houghton,

Kashanian,

Derrien

et al. 2024

ACS Photonics

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Optical microcavities, specifically, whispering-gallery mode (WGM) microcavities, with their remarkable sensitivity to environmental changes, have been extensively employed as biosensors, enabling the detection of a wide range of biomolecules and nanoparticles. To push the limits of detection down to the most sensitive single-molecule level, plasmonic nanorods are strategically introduced to enhance the evanescent fields of WGM microcavities. This advancement of optoplasmonic WGM sensors allows for the detection of single molecules of a protein, conformational changes, and even atomic ions, marking significant contributions in single-molecule sensing. This Perspective discusses the exciting research prospects in optoplasmonic WGM sensing of single molecules, including the study of enzyme thermodynamics and kinetics, the emergence of thermooptoplasmonic sensing, the ultrasensitive single-molecule sensing on WGM microlasers, and applications in synthetic biology.

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Wavelength shift in a whispering gallery microdisk due to bacterial sensing: A theoretical approach

Cited by 12 publications

References 23 publications

Real-Time Detection of Staphylococcus Aureus Using Whispering Gallery Mode Optical Microdisks

Real-Time Detection of Staphylococcus Aureus Using Whispering Gallery Mode Optical Microdisks

Whispering-Gallery Sensors

Whispering-Gallery Mode Optoplasmonic Microcavities: From Advanced Single-Molecule Sensors and Microlasers to Applications in Synthetic Biology

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