Wavelength-encoded laser particles for massively multiplexed cell tagging

Martino, Nicola; Kwok, Sheldon J. J.; Liapis, Andreas C.; Forward, Sarah; Jang, Hoon; Kim, Hwi-Min; Wu, Sarah; Wu, Jiamin; Dannenberg, Paul H.; Jang, Sun‐Joo; Lee, Jun Young; Yun, Seok Hyun

doi:10.1038/s41566-019-0489-0

Cited by 125 publications

(176 citation statements)

References 46 publications

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“…Alternatively, size monitoring could be performed by spectroscopy of reflected white light, 36 which can be applied for smaller droplets, even below 1 μm. Recently, multi-cell tagging was demonstrated by the introduction of polymer beads 25 or micrometer sized semiconductor disks 26 into the cell interior. However, cells are uptaking the microlasers randomly and therefore cell tagging is happening indirectly.…”

Section: Discussionmentioning

confidence: 99%

“…Each bead with slightly different size has a unique emission spectrum, which can be used as a fingerprint to tag samples, products, small particles and even single cells. 19,[25][26][27][28] Most work done till now with WGM barcodes used randomly sized spheres and discs, so that the barcodes were also random. One of the reasons for using random sizes is the difficulty in controlling the size precisely enough to produce an appreciable number of unique barcodes.…”

Section: Introductionmentioning

confidence: 99%

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Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

2020

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

2020

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“…With an optimized delivery range, it will be very interesting to deliver the newly developed intracellular laser particles for intracellular tagging and tracking. [ 175 ]…”

Section: Future Perspectivementioning

confidence: 99%

Intracellular Labeling with Extrinsic Probes: Delivery Strategies and Applications

Liu

Fraire

Smedt

et al. 2020

Small

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Extrinsic probes have outstanding properties for intracellular labeling to visualize dynamic processes in and of living cells, both in vitro and in vivo. Since extrinsic probes are in many cases cell‐impermeable, different biochemical, and physical approaches have been used to break the cell membrane barrier for direct delivery into the cytoplasm. In this Review, these intracellular delivery strategies are discussed, briefly explaining the mechanisms and how they are used for live‐cell labeling applications. Methods that are discussed include three biochemical agents that are used for this purpose—purpose‐different nanocarriers, cell penetrating peptides and the pore‐foraming bacterial toxin streptolysin O. Most successful intracellular label delivery methods are, however, based on physical principles to permeabilize the membrane and include electroporation, laser‐induced photoporation, micro‐ and nanoinjection, nanoneedles or nanostraws, microfluidics, and nanomachines. The strengths and weaknesses of each strategy are discussed with a systematic comparison provided. Finally, the extrinsic probes that are reported for intracellular labeling so‐far are summarized, together with the delivery strategies that are used and their performance. This combined information should provide for a useful guide for choosing the most suitable delivery method for the desired probes.

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“…Microlasers have emerged as a promising technology, garnering a tremendous amount of attention owing to its potential for use in biomedical and biological applications . Various types of optical microcavities have been developed, such as Fabry–Perot cavities, photonic crystals, and whispering‐gallery‐modes (WGMs), as implemented in ring resonators, micro‐/nanodisks, and microspheres . In particular, microsphere‐based WGM lasers are appealing candidates for sensing probes owing to their convenience, extremely high Q factor, and potential for application in intracellular and extracellular probes .…”

Section: Introductionmentioning

confidence: 99%

“…Various types of optical microcavities have been developed, such as Fabry–Perot cavities, photonic crystals, and whispering‐gallery‐modes (WGMs), as implemented in ring resonators, micro‐/nanodisks, and microspheres . In particular, microsphere‐based WGM lasers are appealing candidates for sensing probes owing to their convenience, extremely high Q factor, and potential for application in intracellular and extracellular probes . Moreover, analytes can be positioned outside of the optical cavity, where an evanescent field exists at the external interface between the resonant cavity and surrounding medium .…”

Section: Introductionmentioning

confidence: 99%

Lasing‐Encoded Microsensor Driven by Interfacial Cavity Resonance Energy Transfer

Yuan

Wang

Guan

et al. 2020

Advanced Optical Materials

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for use in biomedical and biological applications. [1][2][3][4][5] Various types of optical microcavities have been developed, such as Fabry-Perot cavities, [6][7][8] photonic crystals, [9] and whispering-gallery-modes (WGMs), as implemented in ring resonators, [10][11][12] micro-/nanodisks, [13,14] and microspheres. [3][4][5][15][16][17][18][19][20] In particular, microsphere-based WGM lasers are appealing candidates for sensing probes owing to their convenience, extremely high Q factor, and potential for application in intracellular and extracellular probes. [13,15,16,21,22] Moreover, analytes can be positioned outside of the optical cavity, where an evanescent field exists at the external interface between the resonant cavity and surrounding medium. [23][24][25] At present, most microsphere or droplet-based WGM lasers are considered to be passive-detection devices, as they require physical changes (e.g., refractive indexes) to induce a resonance spectral shift, and thus cannot provide detailed biochemical information. [26][27][28] In contrast, active-detection devices, which employ analytes as the gain medium, can provide more selective and sensitive information about the biospecies. [8,29] Therefore, the ability to utilize a biological gain medium on the external surface of a microsphere cavity will allow us to amplify subtle changes in the gain medium and the resultant spectra, threshold, and lasing modes. However, the overlap factor for the optical mode and gain medium is much lower than the value of the gain within the cavity, and the background fluorescence also interferes with the external cavity sensing, making this amplification a difficult task. Thus, to enable amplification of these subtle changes, we have applied the concept of Förster resonance energy transfer (FRET) at the interface of a droplet-based laser to separate the donor and acceptor at the droplet-surface interface (Figure 1a). FRET is an electrodynamic phenomenon that is highly sensitive to distance, spectral overlap, and the orientation between donor and acceptor molecules. In the past decade, FRET has been used as a powerful tool for providing nanoscale information in many biosensing applications. The performance of FRET is mainly dependent on the design of the donor and acceptor pairs. Several groups recently utilized FRET by using donor molecules as exciton funnels, [30,31] or light-harvesting antennas, [31] to realize a wavelength tunable laser, [30] single molecule nanoprobes, [32] or light redirectioning devices [33] that strongly amplify the emission of acceptor molecules when Microlasers are emerging tools for biomedical applications. In particular, whispering-gallery-mode (WGM) microlasers are promising candidates for sensing at the biointerface owing to their high quality-factor and potential in molecular assays, and intracellular and extracellular detection. However, lasing particles with sensing functionality remain challenging since the overlap between the WGM optical mode and external gain medium is much lower compared to ...

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Wavelength-encoded laser particles for massively multiplexed cell tagging

Cited by 125 publications

References 46 publications

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

Intracellular Labeling with Extrinsic Probes: Delivery Strategies and Applications

Lasing‐Encoded Microsensor Driven by Interfacial Cavity Resonance Energy Transfer

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