Wavelength-dependent optoacoustic imaging probes for NMDA receptor visualisation

Sim, Neil; Gottschalk, Sven; Pál, Róbert; Delbianco, Martina; Degtyaruk, Oleksiy; Razansky, Daniel; Westmeyer, Gil G.; Ntziachristos, Vasilis; Parker, David; Mishra, Anurag

doi:10.1039/c5cc06277b

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…After verifying high-affinity binding of the material to NMDA receptors in cultured mouse motor neurons (NSC-34), contrast agents were injected into the mouse motor cortex in vivo. Using MSOT imaging, it was possible to retrieve not only highly localized activity of selected contrasts (e.g., L1) in vivo, but also show in 3D the reversibility of this interaction, closely related to changes of local glutamatergic activity (Sim et al, 2015). Specific enrichment of the glutamate sensor in the mouse motor cortex was confirmed by post-mortem cryoslicing and epifluorescence microscopy.…”

Section: Multispectral Molecular Optoacoustic Neuroimagingmentioning

confidence: 93%

“…On the neurotransmitter and receptor imaging frontier, Mishra and colleagues recently applied MSOT for visualizing the activity of extracellular glutamate and NMDA receptors, taking advantage of the conjugates of competitive and non-competitive antagonists of NMDA receptors with heptamethine cyaninederived optoacoustic probes (Sim et al, 2015). After verifying high-affinity binding of the material to NMDA receptors in cultured mouse motor neurons (NSC-34), contrast agents were injected into the mouse motor cortex in vivo.…”

Section: Multispectral Molecular Optoacoustic Neuroimagingmentioning

confidence: 99%

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Pushing the Boundaries of Neuroimaging with Optoacoustics

Ovsepian

Olefir

Westmeyer

et al. 2017

Neuron

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With the central ability to visualize a variety of endogenous chromophores and biomarkers or exogenous contrast agents, optoacoustic (photoacoustic) imaging empowers new experimental capabilities for investigating brain mechanisms and functions. Here, the operational principles of optoacoustic neuroimaging are reviewed in conjunction with recent advances enabling high-resolution and real-time observation, which extend beyond the reach of optical imaging methods. Multiple implementations of optoacoustics for monitoring hemodynamics and neuro-vascular responses in the brain are showcased. The unique capabilities of optoacoustic imaging for multi-spectral cellular and molecular sensing are discussed with reference to recent application for visualizing healthy and diseased brains. Outstanding challenges in the field are considered in the context of current and future applications of optoacoustic neuroimaging for basic and translational neuroscience research. In pushing the boundaries of brain imaging, optoacoustic methods afford major insights into the neuronal mechanisms of brain functions and organization of behavior.

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Section: Multispectral Molecular Optoacoustic Neuroimagingmentioning

confidence: 93%

Section: Multispectral Molecular Optoacoustic Neuroimagingmentioning

confidence: 99%

Pushing the Boundaries of Neuroimaging with Optoacoustics

Ovsepian

Olefir

Westmeyer

et al. 2017

Neuron

Self Cite

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“…Depending on the investigated disease model or organ of interest, alternative administration routes may include subcutaneous, intratumoral or intracranial injections. The latter is important in brain imaging applications to label neurons or other cells that are otherwise unreachable with intravenous administration due to the high restrictiveness of the BBB [218]. The BBB can be overcome via an invasive procedure in which a small hole is drilled into the skull.…”

Section: Dynamic Contrast Enhancement Approachesmentioning

confidence: 99%

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

et al. 2017

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Visualization of dynamic functional and molecular events in an unperturbed in vivo environment is essential for understanding the complex biology of living organisms and of disease state and progression. To this end, optoacoustic (photoacoustic) sensing and imaging have demonstrated the exclusive capacity to maintain excellent optical contrast and high resolution in deep-tissue observations, far beyond the penetration limits of modern microscopy. Yet, the time domain is paramount for the observation and study of complex biological interactions that may be invisible in single snapshots of living systems. This review focuses on the recent advances in optoacoustic imaging assisted by smart molecular labeling and dynamic contrast enhancement approaches that enable new types of multiscale dynamic observations not attainable with other bio-imaging modalities. A wealth of investigated new research topics and clinical applications is further discussed, including imaging of large-scale brain activity patterns, volumetric visualization of moving organs and contrast agent kinetics, molecular imaging using targeted and genetically expressed labels, as well as three-dimensional handheld diagnostics of human subjects.

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“…A dual-modality OFI/PAI probe can be constructed from a single fluorophore 114 , 115 . Generally, NIR dyes with a high molar extinction coefficient and a moderate fluorescence quantum yield are selected as the fluorophore for better tissue penetration.…”

Section: Design and Study Of Multimodality Ofi Probesmentioning

confidence: 99%

Recent developments in multimodality fluorescence imaging probes

Zhao

Chen

et al. 2018

Acta Pharmaceutica Sinica B

185

142

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Multimodality optical imaging probes have emerged as powerful tools that improve detection sensitivity and accuracy, important in disease diagnosis and treatment. In this review, we focus on recent developments of optical fluorescence imaging (OFI) probe integration with other imaging modalities such as X-ray computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), single-photon emission computed tomography (SPECT), and photoacoustic imaging (PAI). The imaging technologies are briefly described in order to introduce the strengths and limitations of each techniques and the need for further multimodality optical imaging probe development. The emphasis of this account is placed on how design strategies are currently implemented to afford physicochemically and biologically compatible multimodality optical fluorescence imaging probes. We also present studies that overcame intrinsic disadvantages of each imaging technique by multimodality approach with improved detection sensitivity and accuracy.

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Wavelength-dependent optoacoustic imaging probes for NMDA receptor visualisation

Abstract: The cellular localisation and binding specificity of two NMDAR-targeted near-IR imaging probes has been examined by microscopy, followed by exemplification of MSOT to monitor simulated glutamate bursts in cellulo and a preliminary study in mice observing the signal in the brain.

Cited by 9 publications

References 17 publications

Pushing the Boundaries of Neuroimaging with Optoacoustics

Pushing the Boundaries of Neuroimaging with Optoacoustics

Advanced optoacoustic methods for multiscale imaging of in vivo dynamics

Recent developments in multimodality fluorescence imaging probes

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