Wavelength-dependent induction of thymine dimers and growth rate reduction in the marine diatom Cyclotella sp. exposed to ultraviolet radiation

Buma, Anita G. J.; Engelen, Aschwin H.; Gieskes, W.W.C.

doi:10.3354/meps153091

Cited by 79 publications

(61 citation statements)

References 22 publications

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“…The formation of photoproducts disturbs the error-free DNA replication and RNA transcription in cells, which is one of the most important consequences of DNA damage (Karentz et al 1994). As shown previously, DNA damage (by CPDs) is only caused by UVBR (Buma et al 1997, Visser et al 1999. However, inhibition of leucine and thymidine incorporation is also caused by UVAR and PAR (Herndl et al 1993, Aas et al 1996, Visser et al 1999).…”

Section: Introductionmentioning

confidence: 62%

“…The formation of CPDs by UVR in bacterioplankton was shown by Jeffrey et al (1996a,b), Lyons et al (1998) and Visser et al (1999), in the marine diatom Cyclotella sp. by Buma et al (1997) and in phagotrophic protists by Somaruga & Buma (2000). The formation of photoproducts disturbs the error-free DNA replication and RNA transcription in cells, which is one of the most important consequences of DNA damage (Karentz et al 1994).…”

Section: Introductionmentioning

confidence: 97%

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Diurnal variations in depth profiles of UV-induced DNA damage and inhibition of bacterioplankton production in tropical coastal waters

Visser¹,

Poos²,

Scheper³

et al. 2002

Mar. Ecol. Prog. Ser.

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In this study, diurnal changes in bacterial production and DNA damage in bacterioplankton (measured as cyclobutane pyrimidine dimers, CPDs) incubated in bags at different depths in tropical coastal waters were investigated. The DNA damage and inhibition of the bacterial production was highest at the surface and decreased with depth. Inhibition of both leucine (Leu) and thymidine (TdR) incorporation was detectable to a depth of 10 m, while DNA damage was measurable only until 5 m. In addition, the decrease of the inhibition of Leu and TdR incorporation with depth was less than the decrease in DNA damage. Variations in DNA damage in bacteria and in biodosimeters were mostly explained by UV radiation (UVR) dose received over time and depth. UVB radiation (UVBR) contributed more than UVA radiation (UVAR) to the damage. The attenuation coefficient of DNA damage with depth was comparable to the attenuation coefficients of UVBR suggesting that DNA damage was induced mainly by UVBR. For incubations at the surface, there was a trend of increasing DNA damage at a constant rate during the entire period of daylight, while the inhibition of leucine incorporation showed a trend of a less rapid increase in the afternoon than in the morning. This might have been due to photorepair, which would also explain the lack of correlation between the inhibition of leucine incorporation and UV dose. It was concluded that CPD damage in DNA is not necessarily the predominant factor in the inhibition of bacterial production. It cannot explain the large impact of sunlight on bacterioplankton productivity in tropical waters. KEY WORDS: DNA damage · Bacterial production · UV radiationResale or republication not permitted without written consent of the publisher

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Section: Introductionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 97%

Diurnal variations in depth profiles of UV-induced DNA damage and inhibition of bacterioplankton production in tropical coastal waters

Visser¹,

Poos²,

Scheper³

et al. 2002

Mar. Ecol. Prog. Ser.

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“…One of the major targets is the DNA, which strongly absorbs in the short-wavelength range of solar radiation. Solar UV-B has been found to induce DNA damage and DNA synthesis delay in many organisms [98][99][100][101]. UV-B effects have also been studied on the ecosystem level using mesocosms [102,103].…”

Section: Phytoplanktonmentioning

confidence: 99%

Effects on aquatic ecosystems

Häder

Kumar

Smith

et al. 1998

Journal of Photochemistry and Photobiology B: Biology

332

160

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Regarding the effects of UV-B radiation on aquatic ecosystems, recent scientific and public interest has focused on marine primary producers and on the aquatic web, which has resulted in a multitude of studies indicating mostly detrimental effects of UV-B radiation on aquatic organisms. The interest has expanded to include ecologically significant groups and major biomass producers using mesocosm studies, emphasizing species interactions. This paper assesses the effects of UV-B radiation on dissolved organic matter, decomposers, primary and secondary producers, and briefly summarizes recent studies in freshwater and marine systems.Dissolved organic carbon (DOC) and particulate organic carbon (POC) are degradation products of living organisms. These substances are of importance in the cycling of carbon in aquatic ecosystems. UV-B radiation has been found to break down high-molecular-weight substances and make them available to bacterial degradation. In addition, DOC is responsible for short-wavelength absorption in the water column. Especially in coastal areas and freshwater ecosystems, penetration of solar radiation is limited by high concentrations of dissolved and particulate matter. On the other hand, climate warming and acidification result in faster degradation of these substances and thus enhance the penetration of UV radiation into the water column.Several research groups have investigated light penetration into the water column. Past studies on UV penetration into the water column were based on temporally and spatially scattered measurements. The process of spectral attenuation of radiant energy in natural waters is well understood and straightforward to model. Less known is the spatial and temporal variability of in-water optical properties influencing UV attenuation and there are few long-term observations. In Europe, this deficiency of measurements is being corrected by a project involving the development of a monitoring system (ELDONET) for solar radiation using three-channel dosimeters (UV-A, UV-B, PAR) that are being installed from Abisko (North Sweden, 688N, 198E) to Tenerife (Canary Islands, 278N, 178W). Some of the instruments have been installed in the water column (North Sea, Baltic Sea, Kattegat, East and Western Mediterranean, North Atlantic), establishing the first network of underwater dosimeters for continuous monitoring.Bacteria play a vital role in mineralization of organic matter and provide a trophic link to higher organisms. New techniques have substantially changed our perception of the role of bacteria in aquatic ecosystems over the recent past and bacterioplankton productivity is far greater than previously thought, having high division and turnover rates. It has been shown that bacterioplankton play a central role in the carbon flux in aquatic ecosystems by taking up DOC and remineralizing the carbon. Bacterioplankton are more prone to UV-B stress than larger eukaryotic organisms and, based on one study, produce about double the amount of cyclobutane dimers. Recently, th...

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“…As a consequence, inter-specific differences in sensitivity to UV-B (Helbling et al 1995, Buma et al 1997, Visser et al 2002, UV-A (Sieracki & Sieburth 1986, Wilhelm & Smith 2000, and PAR , Sommaruga et al 1997, Morán & Zepp 2000, as well as differences in recovery mechanisms from previous stress (Arrieta et al 2000, Gustafson et al 2000, Winter et al 2001, generate changes in community composition that might have important biogeochemical consequences.…”

Section: Introductionmentioning

confidence: 99%

Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile

Hernández¹,

Quiñones²,

Daneri³

et al. 2006

Mar. Ecol. Prog. Ser.

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The influence of solar radiation on bacterial secondary production (BSP), bacterial growth efficiency (BGE), and growth rates (μ) in upwelling zones of the Humboldt Current System (HCS) is not yet understood. Here we assessed the impact of solar radiation, with special focus on ultraviolet radiation (UVR, 280 to 400 nm) on 2 natural bacterioplankton assemblages with different light history coming from surface waters (0.5 m) and subsurface waters (80 m) off central-southern Chile (36°S). The samples were incubated under ambient irradiance for 4 to 11 h and exposed to 3 spectral radiation treatments: photosynthetically active radiation (PAR, 400 to 700 nm), PAR+UV-A (320 to 700 nm), and PAR+UVR (280, to 700 nm). BSP was estimated by 14 C-leucine (protein synthesis) and [methyl-3 H]-thymidine (DNA synthesis) uptake. Both bacterioplankton assemblages showed significant (p < 0.05) inhibition of BSP, BGE, and μ rates when exposed to PAR radiation; in contrast, responses to UV radiation were more variable. At noon, BSP inhibition was 49 to 53% (PAR), 13 to 30% (UV-A), and 5 to 14% (UV-B) for both assemblages. At sunset, in surface assemblages, protein and DNA synthesis were more limited by UV-A than by UV-B, whereas protein synthesis in the subsurface assemblage was more inhibited by UV-B than by UV-A. The same inhibition patterns were found for BGE and μ, especially with regard to protein synthesis. The daily inhibition of BSP, BGE, and μ in both assemblages was mainly a function of PAR followed by UV-A and UV-B. Our results suggest that solar radiation could play an important role in modulating bacterioplankton dynamics (especially protein synthesis) during active upwelling periods in the HCS.

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Wavelength-dependent induction of thymine dimers and growth rate reduction in the marine diatom Cyclotella sp. exposed to ultraviolet radiation

Cited by 79 publications

References 22 publications

Diurnal variations in depth profiles of UV-induced DNA damage and inhibition of bacterioplankton production in tropical coastal waters

Diurnal variations in depth profiles of UV-induced DNA damage and inhibition of bacterioplankton production in tropical coastal waters

Effects on aquatic ecosystems

Effects of solar radiation on bacterioplankton production in the upwelling system off central-southern Chile

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