The duraplasty is a standard procedure during neurosurgery for injuries and diseases of the brain. The hermetic closure of the dura mater is not always possible with the application of autologous tissues. Synthetic, allogeneic and xenogenic implants, which are currently used, have disadvantages, so the search for the material that would best meet the requirements for a dura mater scaffold continues. The purpose is to study the physical and chemical properties of the composite chitosan/polyethylene oxide (PEO) film and determine the effectiveness of its application for duraplasty in the experiment in vivo; to analyze its ability to biodegradation; to evaluate the effect of chitosan/PEO scaffold on the regeneration of dura matter.