Watershed-scale Variation in Potential Fungal Community Contributions to Ectomycorrhizal Biogeochemical Syndromes

Seyfried, Georgia S.; Corrales, Adriana; Kent, Angela D.; Dalling, James W.; Yang, Wendy H.

doi:10.1007/s10021-022-00788-z

Cited by 3 publications

(6 citation statements)

References 73 publications

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“…Therefore, competition for N between host-associated ECM and free-living SAP may not play a role in suppressing N cycling beneath ECM trees. However, it is possible that higher ECM:SAP ratios, greater organic horizon formation and lower quality O. mexicana leaf litter inputs in monodominant O. mexicana stands compared to mixed species stands composed of O. mexicana and AM-associated tree species (Seyfried et al 2022) have the potential to drive interguild competition for N and SAP N limitation. Therefore, although we show that conditions in our tropical forest may decrease interguild competition and its role in suppressing C and N cycling beneath ECM trees relative to neighboring AM trees, we recognize the potential for this mechanism to occur at higher levels of ECM dominance.…”

Section: Soil Chemical and Physical Propertiesmentioning

confidence: 99%

“…Greater belowground C allocation may increase ECM colonization on host roots (e.g., Högberg et (Corrales et al 2017;Guo et al 2021). This can alter interactions between ECM and the surrounding nutrient environment and ultimately the functional potential of fungal communities to contribute ecosystem N cycling dynamics (Seyfried et al 2022;Hicks Pries et al 2022). Nitrogen availability can also shape the overall fungal community directly, with greater N limitation increasing the competitive advantage ECM fungi have over saprotrophic (SAP) fungi (Bending 2003;Fernandez and Kennedy 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, larger differences in 15 N between leaf litter and soil ( 15 N soil−litter ) may indicate 1) greater reliance of host trees on transfer of N from ECM fungi resulting in leaf litter that is less enriched in 15 N and fungal necromass that is more enriched in 15 N and 2) greater contributions of 15 N-enriched fungal biomass to soil organic matter (SOM) pools (Högberg et al 1999;Kohzu et al 2000;Hobbie and Högberg 2012). A concomitant shift in 15 N soil−litter and fungal community composition may therefore indicate that a change in belowground C allocation by host trees can drive compositional and functional variation in fungal communities (Seyfried et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Carver Biotechnology Center at the University of Illinois at Urbana-Champaign where Fluidigm ampli cation (Fluidigm, San Francisco, USA) of the ITS3/ITS4(White et al 1990) target region of DNA and subsequent Illumina sequencing (MiSeq bulk 2 x 250 bp V2, Illumina, San Diego, USA) were carried out. Speci c methodological information about the PCR ampli cation, sequencing and subsequent bioinformatics is described inSeyfried et al (2022). Brie y, we merged paired-end sequences using FLASH (Fast Length Adjustment of Short reads) (Magoč and Salzberg 2011), quality ltered fastq les using software in the FASTX-Toolkit (Gordon and Hannon 2010), clustered sequences into operational taxonomic units (OTUs) using USEARCH (v. 8.1.1861) and a 99% similarity threshold(Edgar 2010) and, assigned taxonomy to representative sequences for ITS OTU's using the UNITE database version 8.3(Nilsson et al 2019).…”

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confidence: 99%

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Interactions between fungal communities and localized conservative N cycling in mixed mycorrhizal tropical forests

Seyfried,

Edwards,

Dalling

et al. 2023

Preprint

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Background and aims Ectomycorrhizal (ECM) trees and associated fungal communities can interact with underlying nutrient dynamics to suppress carbon (C) and nitrogen (N) cycling locally. Specifically, N availability may dictate interactions between ECM and saprotrophic fungi and belowground C allocation by ECM trees, potentially determining the potential of fungal communities to suppress N cycling beneath ECM trees. However, these mechanisms have largely been tested in temperate and boreal systems where underlying N limitation is common.Methods Here, we quantified fungal community metrics and soil chemical properties in a long-term N addition experiment located in a lower montane tropical forest to assess the role of fungal communities in driving conservative N cycling beneath ECM trees within mixed ECM-AM stands where N is not necessarily limiting.Results We found that natural abundance N isotopes correlated with a shift in fungal community composition between control and N addition plots, suggesting that N availability may affect belowground C allocation to increase colonization by ECM fungi that are a larger C investment and have a greater potential to interact with soil N cycling. However, we found no effect of N addition on the distribution of ECM and saprotrophic fungal guilds through the soil profile, suggesting that abiotic and biotic conditions which promote rapid N cycling may decrease interguild competition in a tropical montane forest.Conclusions Overall, local scale nutrient limitation dynamics driven by interactions between ECM host trees and underlying fungal communities may be partially mediated by ecosystem scale nutrient cycling dynamics.

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Section: Soil Chemical and Physical Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Interactions between fungal communities and localized conservative N cycling in mixed mycorrhizal tropical forests

Seyfried,

Edwards,

Dalling

et al. 2023

Preprint

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“…These EMdominated stands often differ signi cantly from surrounding mixed-mycorrhizal forest stands in important ecosystem characteristics (Torti et al 2001), with slower decomposition rates (McGuire et al 2010) and lower soil inorganic nutrient availability (Corrales et al 2016b). Ectomycorrhizal-dominated stands in these forests are also associated with changes to the relative abundance of some fungal functional guilds (Seyfried et al 2022), but the impact of EM-dominance on the broader soil microbiome, particularly prokaryotic communities, is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Soil microbial community response to ectomycorrhizal dominance in diverse neotropical montane forests

Edwards,

Krichels,

Seyfried

et al. 2023

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Ectomycorrhizal (EM) associations can promote the dominance of tree species in otherwise diverse tropical forests. These EM associations between trees and their fungal mutualists have important consequences for soil organic matter cycling, yet the influence of these EM-associated effects on surrounding microbial communities is less well known. We examined fungal and bacterial/ archaeal community composition in surface soil samples from mixed arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) stands as well as stands dominated by EM-associated Oreomunnea mexicana in four adjacent watersheds varying in soil fertility in the Fortuna Forest Reserve, Panama. We hypothesized that EM-dominated stands would support distinct microbial community assemblages relative to the mixed AM-EM stands due to differences in carbon and nitrogen cycling associated with the dominance of EM trees. We expected this microbiome selection in EM-dominated stands would lead to lower overall microbial community diversity and heterogeneity, with tighter correspondence between fungal and bacterial/ archaeal communities. We measured fungal and bacterial/ archaeal community composition via high-throughput Illumina sequencing of the ITS2 (fungi) and 16S rRNA (bacteria/archaeal) gene regions. We analyzed differences in alpha and beta diversity between stand mycorrhizal types, as well as the relative abundance of fungal functional groups and various microbial taxa. We found that fungal and bacterial/ archaeal community composition differed based on stand mycorrhizal type. There was lower bacterial/ archaeal diversity and lower relative abundance of fungal saprotrophs and pathogens in EM-dominant than AM-EM mixed stands. However, contrary to our prediction, there was lower homogeneity for fungal communities in EM-dominated stands compared to mixed EM-AM stands. Overall, we demonstrate that EM-dominated tropical forest stands have distinct soil microbiomes relative to surrounding diverse forests, suggesting that EM fungi may filter microbial functional groups in ways that could potentially influence plant health or ecosystem function.

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Soil microbial community response to ectomycorrhizal dominance in diverse neotropical montane forests

Edwards,

Krichels,

Seyfried

et al. 2024

Mycorrhiza

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Ectomycorrhizal (EM) associations can promote the dominance of tree species in otherwise diverse tropical forests. These EM associations between trees and their fungal mutualists have important consequences for soil organic matter cycling, yet the influence of these EM-associated effects on surrounding microbial communities is not well known, particularly in neotropical forests. We examined fungal and prokaryotic community composition in surface soil samples from mixed arbuscular mycorrhizal (AM) and ectomycorrhizal (EM) stands as well as stands dominated by EM-associated Oreomunnea mexicana (Juglandaceae) in four watersheds differing in soil fertility in the Fortuna Forest Reserve, Panama. We hypothesized that EM-dominated stands would support distinct microbial community assemblages relative to the mixed AM-EM stands due to differences in carbon and nitrogen cycling associated with the dominance of EM trees. We expected that this microbiome selection in EM-dominated stands would lead to lower overall microbial community diversity and turnover, with tighter correspondence between general fungal and prokaryotic communities. We measured fungal and prokaryotic community composition via high-throughput Illumina sequencing of the ITS2 (fungi) and 16S rRNA (prokaryotic) gene regions. We analyzed differences in alpha and beta diversity between forest stands associated with different mycorrhizal types, as well as the relative abundance of fungal functional groups and various microbial taxa. We found that fungal and prokaryotic community composition differed based on stand mycorrhizal type. There was lower prokaryotic diversity and lower relative abundance of fungal saprotrophs and pathogens in EM-dominated than AM-EM mixed stands. However, contrary to our prediction, there was lower homogeneity for fungal communities in EM-dominated stands compared to mixed AM-EM stands. Overall, we demonstrate that EM-dominated tropical forest stands have distinct soil microbiomes relative to surrounding diverse forests, suggesting that EM fungi may filter microbial functional groups in ways that could potentially influence plant performance or ecosystem function.

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Watershed-scale Variation in Potential Fungal Community Contributions to Ectomycorrhizal Biogeochemical Syndromes

Cited by 3 publications

References 73 publications

Interactions between fungal communities and localized conservative N cycling in mixed mycorrhizal tropical forests

Interactions between fungal communities and localized conservative N cycling in mixed mycorrhizal tropical forests

Soil microbial community response to ectomycorrhizal dominance in diverse neotropical montane forests

Soil microbial community response to ectomycorrhizal dominance in diverse neotropical montane forests

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