Watermelon Profilin: Characterization of a Major Allergen as a Model for Plant-Derived Food Profilins

Cases, Bárbara; Pastor-Vargas, Carlos; Gil-Dones, Félix; Pérez‐Gordo, Marina; Maroto, Aroa S.; Heras, Manuel de las; Vivanco, Fernando; Cuesta‐Herranz, Javier

doi:10.1159/000314361

Cited by 17 publications

(15 citation statements)

References 83 publications

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“…Potential allergens, which have been described in other plant and/or non-vegetable sources, could be responsible of allergenic cross-reactivity between different species. For instance, triose-phosphate isomerase, malate hydrogenase and profilins were described as major allergens in watermelon [21,22]; Triose phosphate isomerase was also described in german cockroach ( Blattella germanica ) [23]; Enolase, in latex [24], Candida albicans [25] Sacharomices cerevisiae [26], Aspergillus fumigatus [27], Alternaria alternata Cladosporium herbarum [28] and Blattella germanica [23]; Polygalacturonase, in cuppresaceae [29], tomato [30], and Platanus acerifolia [31] and metylesterase in Salsola kali [32], among others. Interestingly, 1,3-beta-glucanases were identified in both allergen extracts.…”

Section: Discussionmentioning

confidence: 99%

Immunological cross-reactivity between olive and grass pollen: implication of major and minor allergens

Cases

Ibáñez

Tudela

et al. 2014

World Allergy Organization Journal

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BackgroundGrasses and olive trees are the most common sources of allergenic pollen worldwide. Although they share some allergens, there are few studies analyzing the in vitro cross-reactivity between them. The aim was to define the cross-reactivity between Olea europaea and Phleum pratense using well-characterized sera of allergic children from Madrid, Spain.Methods66 patients (mean age 10.32+/−4.07 years) were included in the study. All suffered from rhinoconjuntivitis and/or asthma and had a positive skin test and/or specific IgE determination to olive and grass pollen. Serum sIgE to individual allergens was conducted and sIgE against different grass species and olive was also determined by ELISA. Inhibition assays were performed using two serum sources, containing, or not, sIgE to minor allergens. Mass spectrometry analysis was performed in both extracts.Results59/66 (89.39%) children had a positive sIgE determination by ELISA to grasses and 57/66 (86.36%) to olive pollen. There was no significant correlation between sIgE levels to grass and olive. Inhibition assays demonstrated no cross-reactivity between P. pratense and olive pollen when using the pool containing mainly sIgE to major allergens, whereas minimal to moderate cross-reactivity was detected when the serum contained high sIgE titers to minor allergens. Proteomic analyses revealed the presence of 42 common proteins in grasses and olive pollens.ConclusionNo in vitro cross-reactivity was observed when sIgE was mainly directed to major allergens. In our population, sensitization to olive and grasses is not due to cross-reactivity. The contribution of the major allergens seems to be determinant.

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Section: Discussionmentioning

confidence: 99%

Immunological cross-reactivity between olive and grass pollen: implication of major and minor allergens

Cases

Ibáñez

Tudela

et al. 2014

World Allergy Organization Journal

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“…SDS-PAGE, Immunoblot Analysis, and Inhibition Assays-SDS-PAGE, immunoblotting, and inhibition assays were carried out as described previously (11). Polyacrylamide concentrations of 14% (w/v) and 5% (w/v) were used for separating and stacking gels, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The plates were coated in duplicate overnight at 4°C with 20 g SH extract/well or 0.5 g of Pho s 21kD/well. ELISAs were carried out as described previously (11).…”

Section: Elisa-formentioning

confidence: 99%

Molecular and Immunological Characterization of the First Allergenic Lipocalin in Hamster

Torres

Heras

Maroto

et al. 2014

Journal of Biological Chemistry

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Background: Exposure to uncommon pets such as a Siberian hamster has been described as a new risk factor for the development of allergic disease. Results: For the first time, we have cloned and characterized an allergen from Siberian hamster. Conclusion:The recombinant allergen shows a similar pattern of IgE binding and biological activity compared with salivary gland extract. Significance: The recombinant form will be useful for clinical applications.

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“…ELISA and ELISA inhibition assays were carried out using 96-well flat-bottom plates (Immulon 4HBX, Thermo Scientific), as previously described [14]. The plates were coated in duplicate overnight at 4°C with 10 µg of mollusk proteins per well diluted in coating buffer (0.05 M carbonatebicarbonate buffer, pH 9.6).…”

Section: Elisa and Elisa Inhibition Studiesmentioning

confidence: 99%

Heterogeneity in Allergy to Mollusks: A Clinical-Immunological Study in a Population From the North of Spain

Azofra

Echechipía

Irazábal

et al. 2017

J Investig Allergol Clin Immunol

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Background: Allergy to mollusks has been the focus of fewer studies than allergy to crustaceans. Furthermore, allergy to mollusks is less well characterized. Objectives: To describe the clinical characteristics of mollusk-allergic patients, to identify the responsible allergens, and to assess crossreactivity. Methods: We performed a prospective multicenter study including 45 patients with mollusk allergy, which was diagnosed based on a suggestive clinical history and a positive skin test result with the agent involved. Fractions were identified using SDS-PAGE and immunoblotting. The proteins responsible were subsequently identified using mass spectrometry. ELISA inhibition studies were performed with mollusks, dust mites, and crustaceans. Results: We found that 25 patients (55%) were allergic to cephalopods, 14 (31%) to bivalves, and 11 (24%) to gastropods. Limpet was the third most frequent cause of allergy (15% of cases). In 31 patients (69%), the manifestation was systemic; 10 (22%) exhibited oral allergy syndrome, and 7 (15%) experienced contact urticaria. Most major allergens were found between 27 kDa and 47 kDa. ELISA inhibition assays revealed a high degree of inhibition of cephalopods and bivalves from all the groups of mollusks, mites, and crustaceans. Mass spectrometry identified tropomyosin, actin, and myosin as the major allergens. Conclusions: Cephalopods, especially squid, are the mollusks that most frequently trigger allergic symptoms. The very frequent occurrence of allergy to limpets is striking, given their low consumption in our area. It is worth highlighting the heterogeneity observed, exemplified by the gastropods. Tropomyosin appears to be responsible for the high cross-reactivity found between mollusks, mites, and crustaceans. Three new mollusk allergens were also identified, namely, actin, enolase, and a putative C1q domain-containing protein.

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Watermelon Profilin: Characterization of a Major Allergen as a Model for Plant-Derived Food Profilins

Cited by 17 publications

References 83 publications

Immunological cross-reactivity between olive and grass pollen: implication of major and minor allergens

Immunological cross-reactivity between olive and grass pollen: implication of major and minor allergens

Molecular and Immunological Characterization of the First Allergenic Lipocalin in Hamster

Heterogeneity in Allergy to Mollusks: A Clinical-Immunological Study in a Population From the North of Spain

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