Waterborne Cu exposure increased lipid deposition and lipogenesis by affecting Wnt/β-catenin pathway and the β-catenin acetylation levels of grass carp Ctenopharyngodon idella

Xu, Yi-Chuang; Zhao, Tao; Wu, Lixiang; Yang, Shui-Bo; Luo, Zhi

doi:10.1016/j.envpol.2020.114420

Cited by 44 publications

(34 citation statements)

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“…The lipid metabolism is controlled by many key enzymes and transcription factors. Among these enzymes, fatty acid synthase (FAS), malic enzyme (ME), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) are important lipogenic enzymes and responsible for the regulation of lipogenesis [ 13 , 14 ]. Carnitine palmitoyl transferase I (CPT I) and hormone sensitive lipase (HSL) are two key enzymes involved in lipolysis [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among these enzymes, fatty acid synthase (FAS), malic enzyme (ME), acetyl-CoA carboxylase (ACC), glucose-6-phosphate dehydrogenase (G6PD), and 6-phosphogluconate dehydrogenase (6PGD) are important lipogenic enzymes and responsible for the regulation of lipogenesis [ 13 , 14 ]. Carnitine palmitoyl transferase I (CPT I) and hormone sensitive lipase (HSL) are two key enzymes involved in lipolysis [ 14 , 15 ]. The intestines also possess a fatty acid transport system, and several proteins, such as fatty acid transport protein (FATP), fatty acid binding protein (FABP), and CD36, mediate the control of fatty acid transport [ 12 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Chen

et al. 2021

IJMS

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Nano-sized zinc oxide (nano-ZnO) affects lipid deposition, but its absorption patterns and mechanisms affecting lipid metabolism are still unclear. This study was undertaken to investigate the molecular mechanism of nano-ZnO absorption and its effects on lipid metabolism in the intestinal tissues of a widely distributed freshwater teleost yellow catfish Pelteobagrus fulvidraco. We found that 100 mg/kg dietary nano-ZnO (H-Zn group) significantly increased intestinal Zn contents. The zip6 and zip10 mRNA expression levels were higher in the H-Zn group than those in the control (0 mg/kg nano-ZnO), and zip4 mRNA abundances were higher in the control than those in the L-Zn (50 mg/kg nano-ZnO) and H-Zn groups. Eps15, dynamin1, dynamin2, caveolin1, and caveolin2 mRNA expression levels tended to reduce with dietary nano-ZnO addition. Dietary nano-ZnO increased triglyceride (TG) content and the activities of the lipogenic enzymes glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and isocitrate dehydrogenase (ICDH), upregulated the mRNA abundances of lipogenic genes 6pgd, fatty acid synthase (fas), and sterol regulatory element binding protein 1 (srebp1), and reduced the mRNA expression of farnesoid X receptor (fxr) and small heterodimer partner (shp). The SHP protein level in the H-Zn group was lower than that in the control and the L-Zn group markedly. Our in vitro study indicated that the intestinal epithelial cells (IECs) absorbed nano-ZnO via endocytosis, and nano-Zn-induced TG deposition and lipogenesis were partially attributable to the endocytosis of nano-ZnO in IECs. Mechanistically, nano-ZnO-induced TG deposition was closely related to the metal responsive transcription factor 1 (MTF-1)-SHP pathway. Thus, for the first time, we found that the lipogenesis effects of nano-ZnO probably depended on the key gene shp, which is potentially regulated by MTF1 and/or FXR. This novel signaling pathway of MTF-1 through SHP may be relevant to explain the toxic effects and lipotoxicity ascribed to dietary nano-ZnO addition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Chen

et al. 2021

IJMS

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“…According to the methods described in our recent publications (14,28) , we used western blot analysis to test protein expression, such as SGLT1, SGLT2, Accepted manuscript…”

Section: Immunoblotting Analysismentioning

confidence: 99%

“…According to the methods described in our recent publications (14,28) , we used Western blot analysis to test protein expression, such as SGLT1, SGLT2, Flag-ChREBP and Ac-Lys of ChREBP. Briefly, cell lysates were prepared with cell lysis buffer for Western blot and immunoprecipitation (BL509A; Biosharp).…”

Section: Immunoblotting Analysismentioning

confidence: 99%

“…Immunofluorescence was used to analyse the expression of SGLT1 and SGLT2 in the IEC, according to our recent publications (5,28) . Briefly, cells were cultured in twenty-four-well plates and incubated with the corresponding treatments for 24 h, washed thrice with PBS and fixed in 4 % paraformaldehyde at room temperature for 10 min.…”

Section: Immunofluorescence Analysismentioning

confidence: 99%

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Phospholipase C signal mediated the glucose-induced changes of glucose absorption and lipid accumulation in the intestinal epithelial cells of yellow catfishPelteobagrus fulvidraco

Zhao

Yang

et al. 2021

Br J Nutr

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In present study, we explored the effects and the underlying mechanisms of phospholipase C (PLC) mediating glucose-induced changes in intestinal glucose transport and lipid metabolism by using U-73122 (a PLC inhibitor). We found that glucose incubation activated the PLC signal, and U-73122 pre-incubation alleviated the glucose-induced increase in plcb2, plce1 and plcg1 mRNA expression. Meanwhile, U-73122 pre-treatment blunted the glucose-induced increased in SGLT 1/2 mRNA and protein expressions. U-73122 pretreatment alleviated the glucose-induced increase in TGs content, bodipy493/503 fluorescence intensity, lipogenic enzymes (G6PD, 6PGD, ME and FAS) activity, and the mRNA expressions of lipogenic genes and related transcription factors (6pgd, g6pd, fas, acca, srebp1 and chrebp) in IECs of yellow catfish. Further research found that U-73122 pre-incubation mitigated the glucose-induced increase in the ChREBP protein expression and the acetylation level of ChREBP in HEK293T cells. Taken together, these data demonstrated that the PLC played a major role in the glucose-induced changes of glucose transport and lipid metabolism and provide a new perspective for revealing the molecular mechanism of glucose-induced changes of intestinal glucose absorption, lipid deposition and metabolism.

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Nanotechnology connecting copper metabolism and tumor therapy

Dong

Zhou

2023

MedComm – Biomaterials and Applications

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Copper (Cu) is an essential trace element in the human body that is involved in the formation of several natural enzymes, such as superoxide dismutase and cyclooxygenase. Due to the high density of the outer electron cloud of Cu, which allows the transfer of multiple electrons, Cu is often used as the catalytic center in various metabolic enzymes. However, both deficiency and excessive accumulation of Cu can result in irreversible damage to cells. Therefore, strategies to regulate Cu metabolism, such as Cu exhaustion and Cu supplementation, have emerged as attractive approaches in anticancer therapy, due to the potential damages caused by Cu metabolism disorders. Notably, recent advancements in nanotechnology have enabled the development of nanomaterials that can regulate Cu metabolism, making this therapy applicable in vivo. In this review, we provide a systematic discussion of the physical and chemical properties of Cu and summarize the applications of nanotechnology in Cu metabolism‐based antitumor therapy. Finally, we outline the future directions and challenges of nano‐Cu therapy, emphasizing the scientific problems and technical bottlenecks that need to be addressed for successful clinical translation.

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Waterborne Cu exposure increased lipid deposition and lipogenesis by affecting Wnt/β-catenin pathway and the β-catenin acetylation levels of grass carp Ctenopharyngodon idella

Cited by 44 publications

References 46 publications

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish

Phospholipase C signal mediated the glucose-induced changes of glucose absorption and lipid accumulation in the intestinal epithelial cells of yellow catfishPelteobagrus fulvidraco

Nanotechnology connecting copper metabolism and tumor therapy

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