Water Thermocline Confirms Susceptibility of Tilapia Cultured in Lakes to Streptococcus agalactiae

Amal, Mohammad Noor Azmai; Saad, Mohd Zamri; Rashid, Zulkafli Abd; Abdullah, Siti Zahrah; Misri, S.; Ramley, B.; Shahidan, H.; Yusoff, Sabri Mohd

doi:10.3923/javaa.2010.2811.2817

Cited by 19 publications

(15 citation statements)

References 10 publications

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“…In 1997, there was heavy mortality of about 300 to 400 g weight tilapia that were kept in floating net cages in Sungai Pahang (Siti-Zahrah et al 2004), the fish showed clinical features of corneal opacity, exophthalmia, erratic swimming and occasional swollen abdomen, with mortality rate of 70%. Other similar outbreaks were recorded in cages of Kenyir, Pedu and Pergau Lakes in Malaysia in the mid year between April to July and the laboratory investigation conducted revealed the causative agent to be Gram-positive bacteria, S. agalactiae (Siti-Zahrah et al 2005; Amal et al 2008). …”

Section: Introductionmentioning

confidence: 66%

Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.)

et al. 2013

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This study investigates the clinicopathological features of acute experimental streptococcosis in red tilapia using various routes of infection; intraperitoneal (IP), immersion (IM) and immersion cut (IC). Twenty four red tilapia in duplicates were inoculated intraperitoneally with 109 CFU/ml of S. agalactiae while another sets: intact, one with sharp cut at the tail end were exposed to bacterial inoculums 109 CFU/ml diluted in water while two groups of control fish were similarly manipulated. Clinical signs were recorded; samples from the gills, brain, eyes and kidneys were also taken for bacterial isolation and histopathology. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) were employed to detect the antigen. The diseased fish showed skin, fin haemorrhages and exophthalmia with obvious signs in IP at 2 hpc followed by IC and IM at 4 hpc. The lesions were noticed earlier in the kidney and most severe in IP. IHC detected antigen as early as PCR and isolation with intense staining in blood vessel lumen and wall, macrophages in choroid, focal haemorrhage in the renal interstitium and meninges especially in IP followed by IC and IM. The immunolocalisation of the antigen described for the first time further explain the pathogenesis of streptococcosis in red tilapia.

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Section: Introductionmentioning

confidence: 66%

Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.)

et al. 2013

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“…Polymerase chain reaction of 16S rDNA of S. agalactiae was used to confirm the identity of the isolates (Amal et al . ). Positive S. agalactiae strains were processed for 16S rDNA sequencing and compared using Codon Code Aligner Software.…”

Section: Isolation Of Streptococcus Agalactiae From Water Sediment Amentioning

confidence: 97%

Transmission of Streptococcus agalactiae from a hatchery into a newly established red hybrid tilapia, Oreochromis niloticus (L.) × Oreochromis mossambicus (Peters), farm

Amal

Zamri‐Saad

Siti-Zahrah³

et al. 2013

Journal of Fish Diseases

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“…population, with highest mortality between March and June (Siti‐Zahrah, Padilah, Azila, Rimatulhana & Shahidan ). Lately, reported outbreaks and infections of S. agalactiae in tilapia in Malaysia has been widespread (Najiah, Lee, Nadirah, Ruhil, Lee, Wendy, Amal, Basiriah & Siti‐Zahrah ; Ali, Hassan, Saleha, Siti‐Khairani & Milud ; Amal, Zamri‐Saad, Siti‐Zahrah, Zulkafli, Misri, Nur‐Nazifah & Shahidan ; Amal, Zamri‐Saad, Zulkafli, Siti‐Zahrah, Misri, Ramley, Shahidan & Sabri ; Zamri‐Saad, Amal & Siti‐Zahrah ; Amal, Zamri‐Saad, Siti‐Zahrah & Zulkafli ).…”

Section: Introductionmentioning

confidence: 99%

Water quality influences the presence ofStreptococcus agalactiaein cage cultured red hybrid tilapia,Oreochromis niloticus × Oreochromis mossambicus

Amal

Saad

Zahrah³

et al. 2013

Aquac Res

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Attempts were made to identify the association between water quality parameters and the presence of Streptococcus agalactiae in cage cultured red hybrid tilapia, Oreochromis niloticus × O. mossambicus. Fish from commercial floating net cage‐culture systems in a river and lake were randomly sampled over a 24‐month period. Swabs from the brains, eyes and kidneys were streaked directly onto blood agar to isolate S. agalactiae. Water temperature, dissolved oxygen, pH, clarity, ammonia, nitrite, sulfide, rate of water flow and depth of water at sampling sites were measured at the same time of fish sampling. The prevalence of fish that were cultured positive to S. agalactiae was significantly higher in lake compared with river. The length and weight of the infected fish were between 9 and 33 cm, and between 20 and 760 g respectively. There was a significant and positive strong correlation between the presence of S. agalactiae and fish mortalities in lake. All water quality parameters showed significant differences between river and lake. However, only water temperature, clarity and pH of lake and the ammonia, temperature and dissolved oxygen in river showed significant correlation with the presence of S. agalactiae in the cultured fish. It was concluded that several unfavourable water quality in the fish farm influencing the presence of S. agalactiae in cultured red hybrid tilapia.

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Water Thermocline Confirms Susceptibility of Tilapia Cultured in Lakes to Streptococcus agalactiae

Cited by 19 publications

References 10 publications

Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.)

Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.)

Transmission of Streptococcus agalactiae from a hatchery into a newly established red hybrid tilapia, Oreochromis niloticus (L.) × Oreochromis mossambicus (Peters), farm

Water quality influences the presence ofStreptococcus agalactiaein cage cultured red hybrid tilapia,Oreochromis niloticus × Oreochromis mossambicus

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