Water Structure and Blood Compatibility of Poly(tetrahydrofurfuryl acrylate)

Abstract

We previously reported that poly(2-methoxyethyl acrylate) (PMEA), which has excellent blood compatibility, contains a large amount of freezing bound water. In order to confirm the role of freezing bound water in determining blood compatibility, poly(tetrahydrofurfuryl acrylate) (PTHFA) was newly synthesized and the thermal properties of water in PTHFA were investigated by differential scanning calorimetry (DSC), as freezing bound water was observed as cold crystallization in DSC heating curves. In addition, the blood compatibility of PTHFA, including activations of platelets, the coagulation system and the complement system, was investigated. The temperature of cold crystallization of water in PTHFA was higher than that of water in PMEA; moreover, the amount of freezing bound water in PTHFA was smaller than that in PMEA. The effect of freezing bound water on blood compatibility was investigated by comparing PTHFA, PMEA, poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(2-methoxyethyl methacrylate) (PMEMA). The latter two samples showed no cold crystallization. Activations of platelets, the coagulation system and the complement system were enhanced in the following order: PMEA < PHEMA < PTHFA < PMEMA, PMEA < PMEMA < PTHFA < PHEMA and PMEA < PTHFA < PMEMA < PHEMA, respectively. The above results were reasonably explained by the amount and/or the stability of freezing bound water.

Characterization of the Attachment Mechanisms of Tissue‐Derived Cell Lines to Blood‐Compatible Polymers

“…In the case of MDA‐MB‐231 and MCF‐7 cell attachment, the cells attached to the PMEA substrate via integrin‐dependent and ‐independent mechanisms. On the PMEA substrate, proteins are hardly adsorbed . In fact, the amounts of bovine serum albumin (BSA) adsorbed to the PMEA, PTHFA, and PSt substrates were measured using quartz crystal microbalance (QCM) after 15 min and were found to be 13.7 ± 4.0 ng cm −2 , 374.2 ± 14.3 ng cm −2 , and 282.2 ± 27.8 ng cm −2 , respectively.…”

“…Protein conformational changes affect various cell functions, including cell differentiation . Intermediate water in hydrated polymers plays an important role in the suppression of protein deformation . Therefore, it may be possible to regulate cell functions using blood‐compatible polymers by controlling the intermediate water content.…”

“…We previously identified poly(2‐methoxyethyl acrylate) (PMEA) and its analogous polymer, poly(tetrahydrofurfuryl acrylate) (PTHFA), as blood‐compatible polymers . PMEA and PTHFA substrates prevent platelet attachment through the suppression of fibrinogen adsorption and adsorption‐induced deformation, which enables platelets to attach and activate .…”

Optimization of the tissue source, malignancy, and initial substrate of tumor cell-derived matrices to increase cancer cell chemoresistance against 5-fluorouracil

“…PMEA and PTHFA were synthesized according to previous reports [14,15]. PMEA and PTHFA were dissolved in methanol and methanol/chloroform (5:1) at a concentration of 0.2 wt%, respectively.…”

Photopolymerization of acrylates by enzymatically synthesized PCL based macrophotoinitiator

“…Hindering unpleasant odors, providing better solubility and compatibility are also the encouraging factors for utilizing polymeric photoinitiators [11]. Poly(benzyl acrylate) (PBzA) was preferred in the literature due to its high thermal and photochemical stability and poly(tetrahydrofurfuryl acrylate) (PTHFA) was noted with its recognizable blood compatibility [21,22]. Homopolymer and copolymers of 2-hydroxyethyl acrylate (HEA) were widely used in biomedical applications as a result of their hydrogel form, tunable properties and biocompatibility [23,24].…”