Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP

Tezara, Wilmer; Mitchell, V. J.; Driscoll, S. D.; Lawlor, D. W.

doi:10.1038/44842

Cited by 812 publications

(684 citation statements)

References 24 publications

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“…The number of transcripts down-regulated in response to dehydration was less than that seen during salt stress, with most of them having basic biosynthetic functions, mainly in carbohydrate metabolism (Table 6). These results are consistent with previous reports that water stress inhibits photosynthesis (Tezara et al 1999;Ozturk et al 2002).…”

Section: Resultssupporting

confidence: 94%

Changes in gene expression in maize kernel in response to water and salt stress

Andjelkovic

Thompson

2005

Plant Cell Rep

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Increasing pressure on limited water resources for agriculture, together with the global temperature increase, highlight the importance of breeding for droughttolerant cultivars. A better understanding of the molecular nature of drought stress can be expected through the use of genomics approaches. Here, a macroarray of ≈2500 maize cDNAs was used for determining transcript changes during water-and salt-stress treatments of developing kernels at 15 days after pollination. Normalization of relative transcript abundances was carried out using a human nebulin control sequence. The proportions of transcripts that changed significantly in abundance upon treatment (>2-fold compared to the control) were determined; 1.5% of the sequences examined were up-regulated by high salinity and 1% by water stress. Both stresses induced 0.8% of the sequences. These include genes involved in various stress responses: abiotic, wounding and pathogen attack (abscisic acid response binding factor, glycine and proline-rich proteins, pathogenesis-related proteins, etc.). The proportion of down-regulated genes was higher than that for up-regulated genes for water stress (3.2%) and lower for salt stress (0.7%), although only eight

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Section: Resultssupporting

confidence: 94%

Changes in gene expression in maize kernel in response to water and salt stress

Andjelkovic

Thompson

2005

Plant Cell Rep

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“…In other plants and fungi, the ATPase and ATP synthesis pathway have shown to be suppressed under different stress treatments [78,79]. However, KEGG assignment illustrates that most metabolic pathways were active in E. pusillum under PEG-induced stress ( Figure 2).…”

Section: Drought-adaptive Mechanisms In E Pusillummentioning

confidence: 99%

Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms

Wang

Zhang

Zhou

et al. 2014

Sci. China Life Sci.

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The lichen-forming fungus was isolated from the desert lichen Endocarpon pusillum that is extremely drought resistant. To understand the molecular mechanisms of drought resistance in the fungus, we employed RNA-seq and quantitative real-time PCR to compare and characterize the differentially expressed genes in pure culture at two different water levels and with that in desiccated lichen. The comparative transcriptome analysis indicated that a total of 1781 genes were differentially expressed between samples cultured under normal and PEG-induced drought stress conditions. Similar to those in drought resistance plants and non-lichenized fungi, the common drought-resistant mechanisms were differentially expressed in E. pusillum. However, the expression change of genes involved in osmotic regulation in E. pusillum is different, which might be the evidence for the feature of drought adaptation. Interestingly, different from other organisms, some genes involved in drought adaption mechanisms showed significantly different expression patterns between the presence and absence of drought stress in E. pusillum. The expression of 23 candidate stress responsive genes was further confirmed by quantitative real-time PCR using dehydrated E. pusillum lichen thalli. This study provides a valuable resource for future research on lichen-forming fungi and shall facilitate future functional studies of the specific genes related to drought resistance. lichen, mycobiont, dehydration, drought adaption, drought resistant Citation:Wang YY, Zhang XY, Zhou QM, Zhang XL, Wei JC. Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms.

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“…Recent studies have shown that both the photochemical apparatus and CO 2 assimilation capacity are quite resistant to drought stress, and that stomatal closure, with a reduction in mesophyll CO 2 availability, is the main factor responsible for reductions in CO 2 assimilation (stomatal effects) under mild drought (Cornic and Massacci 1996;Chaumont et al 1997;Correia et al 1999). However, other studies suggest a non-stomatal limitation of CO 2 assimilation via a direct effect of drought on ATP synthase, with a reduction of ATP production (Lawlor 1995;Tezara et al 1999) and ribulose-1,5-bisphosphate (RuBP) regeneration (Gunasekera and Berkowitz 1993). The reduction in the maximum photosynthetic capacity under long-term drought allows photosynthesis to operate near break point of the RuBP-and CO 2 -limited regions of the A/C i (where A is net CO 2 Abbreviations: A, net CO 2 assimilation; A max , maximum net CO 2 assimilation; C a , external CO 2 partial pressure; C i , intercellular CO 2 partial pressure; ETp, potential evapotranspiration; FruBPase, fructose-1,6-bisphosphate phosphatase; F v ′/F m ′ , efficiency of PSII open centres; G3PDH, glyceraldehyde-3-phosphate dehydrogenase; g s , stomatal conductance; J max , maximum electron transport rate; PPFD, photosynthetic photon flux density; Q A , primary quinone receptor of PSII; 1-q P , reduction state of the Q A pool; RuBP, ribulose-1,5-bisphosphate; Ru5PK, ribulose-5-phosphate kinase; TPU, triose-P utilization; V Cmax , maximum Rubisco activity; Φ PSII , quantum yield of PSII; Ψ PD , predawn water potential.…”

Section: Introductionmentioning

confidence: 99%

“…There is ongoing discussion on how drought affects photosynthesis, namely on the relative roles of restricted diffusion of CO 2 into the leaf due to stomata closure and inhibition of CO 2 metabolism (Tezara et al 1999;Cornic 2000). Recent studies have shown that both the photochemical apparatus and CO 2 assimilation capacity are quite resistant to drought stress, and that stomatal closure, with a reduction in mesophyll CO 2 availability, is the main factor responsible for reductions in CO 2 assimilation (stomatal effects) under mild drought (Cornic and Massacci 1996;Chaumont et al 1997;Correia et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

Marôco¹,

Rodrigues²,

Lopes³

et al. 2002

Functional Plant Biol.

180

104

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Abstract. The effects of a slowly-imposed drought stress on gas-exchange, chlorophyll a fluorescence, biochemical and physiological parameters of Vitis vinifera L. leaves (cv. Aragonez, syn. Tempranillo) growing in a commercial vineyard (South Portugal) were evaluated. Relative to well-watered plants (predawn water potential, Ψ PD = -0.13 ± 0.01 MPa), drought-stressed plants (Ψ PD = -0.97 ± 0.01 MPa) had lower photosynthetic rates (ca 70%), stomatal conductance, and PSII activity (associated with a higher reduction of the quinone A pool and lower efficiency of PSII open centres). Stomatal limitation to photosynthesis was increased in drought-stressed plants relative to well-watered plants by ca 44%. Modelled responses of net photosynthesis to internal CO 2 indicated that drought-stressed plants had significant reductions in maximum Rubisco carboxylation activity (ca 32%), ribulose-1,5-bisphosphate regeneration (ca 27%), and triose phosphate (triose-P) utilization rates (ca 37%) relative to well-watered plants. There was good agreement between the effects of drought on modelled biochemical parameters, and in vitro activities of key enzymes of carbon metabolism, namely Rubisco, glyceraldehyde-3-phosphate dehydrogenase, ribulose-5-phosphate kinase and fructose-1,6-bisphosphate phosphatase. Quantum yields measured under both ambient (35 Pa) and saturating CO 2 (100 Pa) for drought-stressed plants were decreased relative to well-watered plants, as well as maximum photosynthetic rates measured at light and CO 2 saturating conditions (three times ambient CO 2 levels). Although stomatal closure was a strong limitation to CO 2 assimilation under drought, comparable reductions in electron transport, CO 2 carboxylation, and utilization of triose-P capacities were also adaptations of the photosynthetic machinery to dehydration that slowly developed under field conditions. Results presented in this study confirm that modelling photosynthetic responses based on gas-exchange data can be successfully used to predict metabolic limitations to photosynthesis.

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Water stress inhibits plant photosynthesis by decreasing coupling factor and ATP

Cited by 812 publications

References 24 publications

Changes in gene expression in maize kernel in response to water and salt stress

Changes in gene expression in maize kernel in response to water and salt stress

Comparative transcriptome analysis of the lichen-forming fungus Endocarpon pusillum elucidates its drought adaptation mechanisms

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

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