Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice

Sheoran, I. S.; Koonjul, Priyum; Attieh, Jihad; Saini, Harvinder Singh

doi:10.1016/j.plaphy.2014.04.011

Cited by 11 publications

(7 citation statements)

References 47 publications

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“…Five of these markers co-localized with genes involved in plant height or panicle length. PL-6, in chromosome 3, co-localised with rice panicle 3 ( rip-3 ), coding for an α-tubulin that probably participates in the suppression of the panicle elongation during water deficit [ 27 ]. Semidwarf gene g / gibberellin insensitive dwarf1 ( sdg / gid1 ) co-localised to PL-8, in chromosome 5 (Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…On the other side, PL-6 localized close to rice panicle 3 ( rip-3 ), coding for a putative α-tubulin protein, which expression was observed in all the reproductive organs of rice panicle, but not in other parts of the plant. Interestingly, rip-3 acts, supposedly, as suppressor of panicle elongation in the regions of high growth and in periods of water deficit [ 27 ]. Panicle length is often associated with yield as long panicles may produce higher number of spikelets and, thus, higher number of grains [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide association study of agronomic traits in rice cultivated in temperate regions

Reig-Valiente

Marques²,

Talón

et al. 2018

BMC Genomics

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BackgroundRice plants are sensitive to the agro-climate conditions, being photoperiod one of main factor contributing to their adaptation to the region where they are grown. Dissecting the genetic bases underlying diversity in rice populations adapted to specific environmental conditions is a fundamental resource for breeding. In this study we have analysed a collection of japonica varieties adapted to temperate regions to perform association studies with traits of high agronomical interest such as heading date, plant height, number of panicles, panicle length and number of grains per panicle.ResultsWe have performed a genome wide association study using a panel of 1713 SNPs that, based on previous linkage disequilibrium estimations, provides a full coverage of the whole genome. We have found a total of 43 SNPs associated with variations in the different traits. The identified SNPs were distributed across the genome except in chromosome 12, where no associated SNPs were found. The inspection of the vicinity of these markers also revealed a set of genes associated with physiological functions strongly linked to agronomic traits. Of special relevance are two genes involved in gibberellin homeostasis that are associated with plant height and panicle length. We also detected novel associated sites with heading date, panicle length and number of grain per panicle.ConclusionWe have identified loci associated with important agronomic traits among cultivars adapted to temperate conditions. Some of these markers co-localized with already known genes or QTLs, but the association also provided novel molecular markers that can be of help to elucidate the complicated genetic mechanism controlling important agronomic traits, as flowering regulation in the non-dependent photoperiod pathway. The detected associated markers may provide important tools for the genetic improvement of rice cultivars in temperate regions.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5086-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-wide association study of agronomic traits in rice cultivated in temperate regions

Reig-Valiente

Marques²,

Talón

et al. 2018

BMC Genomics

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“…The tubulin was a basic component of cytoskeleton in all eukaryotic cells and decreased under drought stress in this study. Microtubules were a major structural component of the cytoskeleton and participate in cell division, intracellular transport, and cell morphogenesis (Alieva 2014 ); it could be related to dynamic reorganization of microtubules and polymers of α- and β-tubulin heterodimers (Sheoran et al 2014 ). It showed that tubulin alpha-2 chain (spot 13) expression was downregulated under drought stress, so we speculate that the drought resistance of the cultivar “Ga da dou” may be based on expression changes of cytoskeleton proteins, by regulating cell size to adjust osmotic pressure and showing strong drought resistance.…”

Section: Discussionmentioning

confidence: 99%

Drought stress impact on leaf proteome variations of faba bean (Vicia faba L.) in the Qinghai–Tibet Plateau of China

Zhang

et al. 2018

3 Biotech

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Water scarcity is a major abiotic stress factor that strongly influences growth, development and yield of grain legumes in arid and semi-arid area of the world. Drought stress frequently occurs during the seedling stage and finally affects yield of faba bean (Vicia faba L.). However, the responses of plant leaf to drought have not been documented very well at the proteomic level. “Ga da dou” of the drought-tolerant faba bean cultivar was exposed to drought to examine the proteome changes of leaves. In this study, 2-week-old seedlings were subjected to water deficit by 7 days drought stress, whereas control plants were regularly irrigated. After withdrawing water, plants exposed to drought for 7 days and control plants at the same developmental stage were included in quantitative proteomic analysis using two-dimensional electrophoresis gels of proteins in combination with mass spectrometry. Over 300 proteins were detected by 2-DE, 50 differentially expressed proteins were detected by t test and 30 proteins were successfully identified by MALDI-TOF/TOF, in which 25 proteins were clearly downregulated and five proteins were upregulated. The quantified proteins were grouped into five functional groups, mainly regulatory proteins (46.7%), energy metabolism (23.3%), cell cytoskeleton (6.7%), other functions (20%) and unknown function (3.3%). Chitinase was upregulated under drought, suggesting that it was an important part of the plant defense system, playing an important role in stress resistance. 50S ribosomal protein was upregulated under drought, suggesting its role in protecting plants against stress by re-establishing normal protein conformations. The abundance of proteins involved in protein synthesis such as chitinase, Bet protein and glutamate–glyoxylate aminotransferase was upregulated under drought stress. These proteins could play important roles in drought tolerance and contribute to the relatively stronger drought tolerance of “Ga da dou”.

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“…Cytoskeleton proteins such as actins and tubulins play an important role in developing spikelets. Actins affect cell growth in the spikelets (Hussey et al 2006 ), while tubulins also respond to flowering and spikelet development (Sheoran et al 2014 ; Giani and Brevario 1996 ). An actin and tubulin were found to be more abundant in the spikelets of 481-B than in Vandana through a proteomic study (Raorane et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Variation in primary metabolites in parental and near-isogenic lines of the QTL qDTY 12.1 : altered roots and flag leaves but similar spikelets of rice under drought

et al. 2015

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There is a widespread consensus that drought will mostly affect present and future agriculture negatively. Generating drought-tolerant crops is thus a high priority. However complicated the underlying genetic and regulatory networks for differences in plant performance under stress are, they would be reflected in straightforward differences in primary metabolites. This is because primary metabolites such as amino acids and sugars form the building blocks of all pathways and processes for growth, development, reproduction, and environmental responses. Comparison of such differences was undertaken between the parental line and a near-isogenic line of qDTY12.1, a QTL for rice yield under drought. The comparison was informative regarding the effect of the QTL in three genetic backgrounds: donor, recipient, and improved recipient, thus illustrating the gene × gene (G × G) interactions. Such a comparison when extended to well-watered and drought conditions illustrated the gene × environment (G × E) interactions. Assessment of such G × G and G × E responses in roots, flag leaves, and spikelets added a yet more informative dimension of tissue-specific responses to drought, mediated by qDTY12.1. Data on variation in primary metabolites subjected to ANOVA, Tukey’s test, Welch’s t test, and PCA underscored the importance of the roots and demonstrated concordance between variation in metabolites and morpho-physiological responses to drought. Results suggested that for gainful insights into rice yield under drought, rather than vegetative stage drought tolerance, multiple tissues and genotypes must be assessed at the reproductive stage to avoid misleading conclusions about using particular metabolites or related genes and proteins as candidates or markers for drought tolerance.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0322-5) contains supplementary material, which is available to authorized users.

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Water-stress-induced inhibition of α-tubulin gene expression during growth, and its implications for reproductive success in rice

Cited by 11 publications

References 47 publications

Genome-wide association study of agronomic traits in rice cultivated in temperate regions

Genome-wide association study of agronomic traits in rice cultivated in temperate regions

Drought stress impact on leaf proteome variations of faba bean (Vicia faba L.) in the Qinghai–Tibet Plateau of China

Variation in primary metabolites in parental and near-isogenic lines of the QTL qDTY 12.1 : altered roots and flag leaves but similar spikelets of rice under drought

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