Water stress-induced ethylene in the calyx triggers autocatalytic ethylene production and fruit softening in ‘Tonewase’ persimmon grown in a heated plastic-house

Nakano, Ryohei; Inoue, Shinsuke; Kubo, Yasutaka; Inaba, Akitsugu

doi:10.1016/s0925-5214(02)00009-1

Cited by 66 publications

(32 citation statements)

References 22 publications

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“…Increased ethylene production was found when fruit was detached (Hyodo 1991) and also after having experienced water stress/deficit during its development (Gelly et al 2003). The level of transcription for the genes of ACCsynthase and ACC-oxidase responded positively to the water deficit in a tissue-specific and coordinated manner (Nakano et al 2002). This resulted in enhanced activities of these two enzymes under water stress (Fig.…”

Section: Water Vapours/water Status In Fruitmentioning

confidence: 96%

Role of internal atmosphere on fruit ripening and storability—a review

2011

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Concentrations of different gases and volatiles present or produced inside a fruit are determined by the permeability of the fruit tissue to these compounds. Primarily, surface morphology and anatomical features of a given fruit determine the degree of permeance across the fruit. Species and varietal variability in surface characteristics and anatomical features therefore influence not only the diffusibility of gases and volatiles across the fruits but also the activity and response of various metabolic and physiological reactions/processes regulated by these compounds. Besides the well-known role of ethylene, gases and volatiles; O 2 , CO 2 , ethanol, acetaldehyde, water vapours, methyl salicylate, methyl jasmonate and nitric oxide (NO) have the potential to regulate the process of ripening individually and also in various interactive ways. Differences in the prevailing internal atmosphere of the fruits may therefore be considered as one of the causes behind the existing varietal variability of fruits in terms of rate of ripening, qualitative changes, firmness, shelf-life, ideal storage requirement, extent of tolerance towards reduced O 2 and/or elevated CO 2 , transpirational loss and susceptibility to various physiological disorders. In this way, internal atmosphere of a fruit (in terms of different gases and volatiles) plays a critical regulatory role in the process of fruit ripening. So, better and holistic understanding of this internal atmosphere along with its exact regulatory role on various aspects of fruit ripening will facilitate the development of more meaningful, refined and effective approaches in postharvest management of fruits. Its applicability, specially for the climacteric fruits, at various stages of the supply chain from growers to consumers would assist in reducing postharvest losses not only in quantity but also in quality.

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Section: Water Vapours/water Status In Fruitmentioning

confidence: 96%

Role of internal atmosphere on fruit ripening and storability—a review

2011

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“…This led to a serious economic loss in Wakayama Prefecture. We clarified the mechanism of this fruit softening and developed a completely suppressible technique based on that mechanism (Harima et al, 2001(Harima et al, , 2002a(Harima et al, , 2002bNakano et al, 2001aNakano et al, , 2001bNakano et al, , 2002Nakano et al, , 2003. It is known that the harvested persimmon fruit produces large amounts of ethylene at a younger stage and its rate decreases with growth (Takata, 1983).…”

Section: Clarification Of Mechanism and Development Ofmentioning

confidence: 99%

Studies on the Internal Feedback Regulation of Ethylene Biosynthesis and Signal Transduction during Fruit Ripening, and the Improvement of Fruit Quality

Inaba¹

2007

J. Japan. Soc. Hort. Sci.

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A brief account of research carried out in our laboratory over the past decade on the postharvest ripening of several fruits is given. Physiological and molecular biological studies reviewed include those relating to ethylene biosynthesis and flesh softening during fruit ripening. Work relating to the development of postharvest treatments and handling procedures aimed at maintaining the quality and extending the shelf-life of 'Tonewase' Japanese persimmon fruit during distribution is also summarized.

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“…2C) which suggests that the expression of this gene is regulated in an ethylene-independent manner. However, the expression of this gene was not detected in 'Tonewase' persimmon fruits that were subjected to either low or high humidity or in the presence of 1-MCP (Nakano et al, 2002).…”

Section: Discussionmentioning

confidence: 88%

“…ACO gene expression is regulated by ethylene in many climacteric fruits, such as tomato (Barry et al, 1996) and apples (Dong et al, 1992). In tomato, LE-ACO1 was expressed mainly in ripening fruit (Nakatsuka et al, 1998), whereas PP-ACO1 became abundant in ripening peaches (Mathooko et al, 2001), mume (Mita et al, 1997), and 'Tonewase' persimmon (Nakano et al, 2002). 1-MCP application, caused a transient suppression in the activity of ACC oxidase whereby levels almost dropped twentyfold, but a dramatic increase in activity occurred on day 16, which far exceeded levels in control fruits (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…Characterization and cloning of the genes encoding ACC synthase and ACC oxidase, the major enzymes of the ethylene biosynthetic pathway, in 'Hiratanenashi' and 'Tonewase' persimmon revealed that both enzymes may be developmentally regulated and spatially coordinated (Nakano et al, 2002. In these studies, softening in both young and mature persimmon fruit has been attributed to the action of the ethylene that is produced in the calyx, in response to water stress through the activated expression of DK-ACS2, which in turn, induces an autocatalytic ethylene production in the mesocarp.…”

Section: Introductionmentioning

confidence: 99%

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Expression of 1-Aminocyclopropane-1-Carboxylate Synthase and 1-Aminocyclopropane-1-Carboxylate Oxidase Genes during Ripening in ‘Rendaiji’ Persimmon Fruit

Ortiz¹,

純子²,

由彦³

et al. 2006

J. Japan. Soc. Hort. Sci.

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'Rendaiji' persimmon (Diospyros kaki Thunb.) is a pollination variant astringent type persimmon that is characterized by low levels of the ethylene production rate during ripening. However, a serious problem in postharvest handling is that mature fruits soften quite rapidly accompanied with increased ethylene production after treatments to remove astringency. In this study, we investigated changes in ethylene biosynthesis, by following the accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC) and 1-(malonylamino)-cyclopropane-1-carboxylic acid (MACC), as well as ACC synthase (ACS) and ACC oxidase (ACO) activities and the expression of their respective genes in relation to the process and mechanism of fruit ripening. The response by persimmon fruits to 1-methylcyclopropene (1-MCP) treatments during postharvest ripening was also studied. Ripening was accompanied by relatively low levels of the ethylene production rate, concomitant with a decrease in firmness, especially after exposure to a deastringency treatment by using ethanol vapor. Postharvest application of 1-MCP, on the other hand, did not suppress ethylene production rates to the expected level, but it lowered the accumulation of ACC and the activities of ACC synthase and ACC oxidase. However, the degree of inhibition was higher in ACC synthase, which would imply that the step catalyzed by this enzyme is more subject to regulation by ethylene in this fruit. Comparing gene expression patterns in control and 1-MCP treated fruit by using quantitative RT (Real-Time)-PCR showed that ethylene biosynthesis associated with rapid ripening in 'Rendaiji' was accompanied by the expression of DK-ACS1, DK-ACS2, DK-ACO1, and DK-ACO2 genes. The expression of DK-ACS3, however, was not induced.

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Water stress-induced ethylene in the calyx triggers autocatalytic ethylene production and fruit softening in ‘Tonewase’ persimmon grown in a heated plastic-house

Cited by 66 publications

References 22 publications

Role of internal atmosphere on fruit ripening and storability—a review

Role of internal atmosphere on fruit ripening and storability—a review

Studies on the Internal Feedback Regulation of Ethylene Biosynthesis and Signal Transduction during Fruit Ripening, and the Improvement of Fruit Quality

Expression of 1-Aminocyclopropane-1-Carboxylate Synthase and 1-Aminocyclopropane-1-Carboxylate Oxidase Genes during Ripening in ‘Rendaiji’ Persimmon Fruit

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