Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance

Toumi, Imene; Gargouri, Mahmoud; Nouairi, Issam; Moschou, Panagiotis N.; Salem-Fnayou, Asma Ben; Mliki, Ahmed; Zarrouk, Mokhtar; Ghorbel, A.

doi:10.1007/s10535-008-0035-2

Cited by 93 publications

(70 citation statements)

References 19 publications

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“…Augusta when compared to cv. Aldana [27]. Unlike the carotenoid analysis, FT-Raman spectroscopy did not provide unambiguous results in the analysis of chlorophylls.…”

Section: Evaluation Of Pigment Contentmentioning

confidence: 88%

“…However, these changes very often depend on the genotype and on the type of stress. Toumi et al (2008) [27] studied grapevine under drought stress, and observed changes in the composition of total lipids towards the increase of the proportion of 18:3. The increase in the percentage of 18:3 under the influence of drought was also reported in the study of Gigon et al (2004) [28] in certain lipid fractions (DGDG and phosphatidylcholine fraction) in Arabidopsis thaliana L. What is more, these authors found an overall decrease in the amount of lipids reaching even 75% as a result of drought.…”

Section: Fatty Acid Assaymentioning

confidence: 99%

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FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

et al. 2015

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The aim of study was to evaluate the usefulness of FT-Raman spectroscopy in assessing stress-induced metabolic changes in plants. 20-d-old optimally watered plants of soybean were exposed to drought. Metabolic changes in optimally watered and drought-stressed plants were monitored using FT-Raman spectroscopy. In parallel, analyses were carried out of fatty acid composition and pigment content using analytical methods. These compounds are associated with the response of plants to environmental stress. While fatty acid assays in study were inconclusive, the pigment content analysis gave promising results. FT-Raman experiment demonstrated a decrease in carotenoid content in leaf, as a result of drought, which was confirmed by spectrophotometric analysis. In addition to the analysis of aforementioned compounds, FT-Raman spectroscopy allowed the simultaneous observation of a wider spectrum of compounds scattering the radiation in the leaves tested, and their subsequent comparative analysis. The impact of drought on metabolism of soybean was clearly visible on spectra and confirmed using cluster analysis. The technical problem of the influence of leaf water content on measurements, which appeared in studies, will be discussed. To conclude, FT-Raman spectroscopy may be a good complement to other noninvasive methods, e.g., fluorescent methods, in assessing the stress-induced damage of crops.

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“…Augusta when compared to cv. Aldana [27]. Unlike the carotenoid analysis, FT-Raman spectroscopy did not provide unambiguous results in the analysis of chlorophylls.…”

Section: Evaluation Of Pigment Contentmentioning

confidence: 88%

Section: Fatty Acid Assaymentioning

confidence: 99%

FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

et al. 2015

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“…Being the most widely cultivated perennial plant, grapevine occupies a privileged position and has the greatest economic value worldwide, as well as in Tunisia. However, autochthonous Tunisian grapevine varieties exhibit enhanced osmotic tolerance and adaptive responses to stress conditions [1][2] which represent a valuable resource for studying mechanisms of adaptation to salinity and drought in V. vinifera. Thus understanding the molecular genetic basis of the environmental stress responses would be crucial towards improving grapevines production.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

Daldoul¹,

Hanana²,

Mliki³

2012

Turk J Bioch

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Aim: After identifying and isolating a grapevine alkaline α-galactosidase gene and before initializing functional genomic studies, we judged necessary to acquire minimal knowledge about this enzyme that catalyzes galactose release in the more neutral to alkaline cytoplasm. Our goal was to search and identify structural motives and domains that could provide knowledge and information about protein activity and functionality. Methods: We realized a bioinformatics analysis using several tools based on the homology search, promoter analysis, in order to determine basic characteristics of this protein that could guide us in the functional characterization works. As with several bioinformatics approaches, it is the combination of tools and data resources which provides the best results. Results: We discovered a new alkaline α-gal gene by using Bioinformatics tools. Vv-α-gal/ SIP gene is made of 13 exons and measures 2.9 kb, it encodes a protein of 774 amino acids with a predicted molecular mass of 85 kDa. The enzyme contains several putative abiotic stress regulatory elements. Conclusion: This study reports the first bioinformatic characterization of the Vv-α-gal-SIP gene. We investigated the possibility of predicting the function of Vv-α-gal-SIP only by its sequence. Several regulatory elements were predicted and described to be involved in abiotic stress signaling. The discovery of new alkaline α-Gals may contribute to important technological applications of α-Gals.

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“…A lot of work on the mechanism of drought damage to Pallavi and Rama, 2005;Jan, 2006;Toumi et. al., 2008).…”

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confidence: 99%

The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves

Sun¹,

Wei²,

Xiang-ling³

et al. 2010

Afr. J. Biotechnol.

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A model of drought was created on pigweed and the effects of drought stress on the activity of acid phosphatase and its protective enzymes were examined. The pot-cultured pigweeds were divided into 4 groups (ten plants per group) when they reached 6 leaves. (1) In the control group, the culture media contained 70 -85% of field moisture capacity, (2) In the second group, the mild drought stress group, the culture media contained 50 -60% of field moisture capacity, (3) The moderate drought stress group had a culture media that contained 40 -50% of field moisture capacity; (4) The severe drought stress group culture media contained 30 -40% of field moisture capacity. All through the process of the present study, the pigweed plants were cultured under natural conditions on the rooftop of the laboratory building; though transferred indoor in rainy days to avoid the influence of natural precipitation. The plants were sampled and detected every five days after the administration of drought stress. The results clearly demonstrated that the drought stress significantly enhanced the activity of acid phosphatase, membrane permeability and MDA contents; though the activity of acid phosphatase declined after a certain time of drought stress, the extent of membrane permeability and MDA contents still increased with the time. The membrane permeability and MDA contents were correlated with a correlation coefficient of 0.963, 0.971 and 0.939 under mild, moderate and severe drought stress, respectively. The activity of superoxide dismutase (SOD), peroxide dismutase (POD) and hydrogen peroxidase (CAT) was also enhanced with increase in the intensity of drought stress or the prolongation of drought stress at first and then decreased some time afterwards. It was concluded that drought stress enhanced the activity of acid phosphatase, membrane permeability and MDA in the pigweed plants, which was able to resist a certain drought stress by enhancing the activity of protective enzymes. However, excessive drought stress markedly affected the metabolic systems of enzyme and decreased the activity of enzyme.

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Water stress induced changes in the leaf lipid composition of four grapevine genotypes with different drought tolerance

Cited by 93 publications

References 19 publications

FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

FT-Raman spectroscopy as a tool in evaluation the response of plants to drought stress

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

The effects of drought stress on the activity of acid phosphatase and its protective enzymes in pigweed leaves

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