Water stress induced by mannitol and sodium chloride in soybean cultivars

Neto, Nelson Barbosa Machado; Saturnino, Sérgio Marques; Bomfim, Daniel Carlini; Custódio, Ceci Castilho

doi:10.1590/s1516-89132004000400004

Cited by 47 publications

(23 citation statements)

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“…(Table 1). Both the osmotic and toxic effects of the salts have been implicated in the inhibition of the germination (Allen et al, 1986, Machado Neto et al, 2004. Higher germination percentages obtained from NaCl compared to those of PEG at the same concentrations in the same harvest period proved that the adverse effect of PEG on the germination was due to an osmotic effect rather than specific ion accumulation.…”

Section: Resultsmentioning

confidence: 99%

“…Despite the salt resistance varies according to plant ontogeny, the first exposure of the crop to the salinity stress usually occur at the germination stage in direct sowing and transplanting production (Passam and Kakouriotis, 1994). The salt damage to the seed germination is attributed to various factors such as reduction in water availability, changes in mobilisation of stored reserves and affecting structural organisation of proteins (Foolad and Lin, 1997;Almansouri et al, 2001;Machado Neto et al, 2004). The seeds require higher amount of water uptake during the germination under the salt stress due to the accumulation of the soluble solutes around the seeds which increases the osmotic pressure.…”

Section: Introductionmentioning

confidence: 99%

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Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages

Demir

Mavi

2008

Braz. arch. biol. technol.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages

Demir

Mavi

2008

Braz. arch. biol. technol.

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“…Pôde-se observar que o manitol manteve a germinação independente do potencial utilizado, assim como em Machado Neto et al (2006) (PAULA et al, 1994), soja (SANTOS et al, 1996;MACHADO NETO et al, 2004) e pepino (TORRES et al, 2000).…”

Section: Resultsunclassified

“…Em sementes de feijão, o vigor é mais afetado do que a germinação, quando submetidas a tratamento com NaCl (PAULA et al, 1994;MACHADO NETO et al, 2004). Em soja, o índice de germinação, de vigor das sementes e de massa seca total diminuiu quando as sementes foram expostas à deficiência induzida por solução contendo MgCl 2 (MORAES; MENESES, 2003).…”

Section: Introductionunclassified

“…Diversas podem ser as respostas de tolerância dos organismos aos estresses como a resposta diferencial de cultivares MACHADO NETO et al, 2004;TEIXEIRA et al, 2008), via ativação (NEPOMUCENO et al, 2001VERDOY et al, 2004) ou desativação (NEPOMUCENO et al, 2000) de genes que codificarão proteínas específicas, finalizando com diversas respostas fisiológicas, metabólicas e de desenvolvimento. Entre estas respostas ao estresse, encontramos fatores de transcrição (ZHU, 2001), proteínas de diversas classes, como as BiP (Binding Protein) (KALINSKI et al, 1995), Peroxidases (SIEGEL, 1993), proteínas LEA (Late embryogenesis abundant protein) (VERDOY et al, 2004), proteínas ricas em prolina (PRPs) (VERDOY et al, 2004) e Proteínas de Choque Térmico (Heat Shock Protein -HSPs), as quais deveriam ser específicas ao estresse de calor, porém, também são sintetizadas na presença de estresse hídrico ou como resposta universal ao estresse (VIERLING, 1991), sendo encontradas desde em procariotos até em eucariotos.…”

Section: Introductionunclassified

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Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Coelho¹,

Agostini²,

Guaberto³

et al. 2010

Acta Sci. Agron.

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RESUMO.O feijão (Phaseolus vulgaris L.) é de grande expressão alimentícia. A emergência da cultura é dependente de água e considerada a fase mais crítica. Os objetivos deste trabalho foram simular a deficiência de água no início da germinação em laboratório, em sementes de feijão 'Pérola', utilizando-se: manitol, CaCl 2 , MgCl 2 e NaCl em potenciais de 0; -0,3; -0,6; -0,9 e -1,2 MPa, estabelecidos pela equação de Van't Hoff e avaliar o perfil eletroforético de proteínas totais solúveis por meio de SDS-PAGE. Foram avaliados: germinação, classificação de vigor, massa seca de raiz e de parte aérea e resposta diferencial de expressão de proteínas. O delineamento experimental foi inteiramente casualizado. Os dados foram analisados pela aplicação do teste F, para análise de variância, regressão polinomial para os níveis de potencial osmóticos para cada uma das variáveis fisiológicas estudadas. O bandeamento eletroforético foi avaliado visualmente por imagem dos géis. A simulação do estresse permitiu avaliar a drasticidade do NaCl em todos os parâmetros avaliados e a ausência de proteínas de baixo peso molecular neste osmótico. As proteínas de 110 e 30 kDa foram indicativas de estresse hídrico, mas não do salino.Palavras-chave: Phaseolus vulgaris L., deficiência de água, HSPs.ABSTRACT. Differential protein expression during germination as a result of a water deficit associated with variable osmotic pressure in snap-beans. Snap-beans (Phaseolus vulgaris L.) have a high nutritionally value. Successful seedling emergence is dependent on the availability of water and is considered the most critical phase in plant development. The objectives of this work were to simulate water deficiency at the beginning of the germination period. Snap-bean seeds of the variety 'Pérola' were submitted to osmotic stress under laboratory conditions. Mannitol, CaCl 2 , MgCl 2 and NaCl were used to develop five degrees of osmotic potential: 0, -0.3, -0.6, -0.9 and -1.2 MPa. Van't Hoff's equation was used to calculate the osmotic potential. The protein pattern of the total soluble proteins after electrophoresis was evaluated with SDS-PAGE. The response parameters were evaluated including the germination, the vigour classification, the root dry weight, the shoot dry weight and the resultant differential protein expression. The experimental design was completely randomised. Data were analysed using the F test (ANOVA), and polynomial regression was used for the osmotic potential levels for each parameter. The protein banding pattern was evaluated using gel imaging. The simulation of a water deficiency resulted in the perception of a stress and a subsequent response to NaCl in all of the parameters that were evaluated. The harsh effects of the NaCl were validated as the lack of the expression of low molecular weight proteins. Proteins with molecular weights of 110 and 30 kDa were indicative of water stress but not of salinity.

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Seed Priming as a Sustainable Solution to Mitigate Salinity and Drought Stress in Plants

Chakraborty

Roychoudhury

2022

Sustainable Management of Environmental Contaminants

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Water stress induced by mannitol and sodium chloride in soybean cultivars

Abstract: Four soybean (Glycine max L. Merril.)

Cited by 47 publications

References 18 publications

Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages

Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages

Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Seed Priming as a Sustainable Solution to Mitigate Salinity and Drought Stress in Plants

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