Water stress, carbon dioxide, and light effects on sucrose-phosphate synthase activity in Phaseolus vulgaris

Vassey, Terry L.; Quick, W. Paul; Sharkey, Thomas D.; Stitt, Mark

doi:10.1034/j.1399-3054.1991.810106.x

Cited by 35 publications

(30 citation statements)

References 30 publications

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“…According to previous reports, one of the effects of reduced CO 2 assimilation, in spite of a decrease in assimilate utilization by growth, is the reduction of starch, as well as soluble sugars, in leaves of drought-stressed plants (Sharkey and Seeman 1989;Rodrigues et al 1993;Lawlor 1995). This is consistent with the reduction of FruBPase activity observed in drought-stressed leaves of grapevine (this study) and in sucrose phosphate synthase activity observed by Vassey et al (1991) in Phaseolus vulgaris. Our model estimates of C 3 photosynthesis ( Fig.…”

Section: Discussionsupporting

confidence: 80%

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

Marôco¹,

Rodrigues²,

Lopes³

et al. 2002

Functional Plant Biol.

180

104

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Abstract. The effects of a slowly-imposed drought stress on gas-exchange, chlorophyll a fluorescence, biochemical and physiological parameters of Vitis vinifera L. leaves (cv. Aragonez, syn. Tempranillo) growing in a commercial vineyard (South Portugal) were evaluated. Relative to well-watered plants (predawn water potential, Ψ PD = -0.13 ± 0.01 MPa), drought-stressed plants (Ψ PD = -0.97 ± 0.01 MPa) had lower photosynthetic rates (ca 70%), stomatal conductance, and PSII activity (associated with a higher reduction of the quinone A pool and lower efficiency of PSII open centres). Stomatal limitation to photosynthesis was increased in drought-stressed plants relative to well-watered plants by ca 44%. Modelled responses of net photosynthesis to internal CO 2 indicated that drought-stressed plants had significant reductions in maximum Rubisco carboxylation activity (ca 32%), ribulose-1,5-bisphosphate regeneration (ca 27%), and triose phosphate (triose-P) utilization rates (ca 37%) relative to well-watered plants. There was good agreement between the effects of drought on modelled biochemical parameters, and in vitro activities of key enzymes of carbon metabolism, namely Rubisco, glyceraldehyde-3-phosphate dehydrogenase, ribulose-5-phosphate kinase and fructose-1,6-bisphosphate phosphatase. Quantum yields measured under both ambient (35 Pa) and saturating CO 2 (100 Pa) for drought-stressed plants were decreased relative to well-watered plants, as well as maximum photosynthetic rates measured at light and CO 2 saturating conditions (three times ambient CO 2 levels). Although stomatal closure was a strong limitation to CO 2 assimilation under drought, comparable reductions in electron transport, CO 2 carboxylation, and utilization of triose-P capacities were also adaptations of the photosynthetic machinery to dehydration that slowly developed under field conditions. Results presented in this study confirm that modelling photosynthetic responses based on gas-exchange data can be successfully used to predict metabolic limitations to photosynthesis.

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Section: Discussionsupporting

confidence: 80%

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

Marôco¹,

Rodrigues²,

Lopes³

et al. 2002

Functional Plant Biol.

180

104

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“…Low SPS activity could reduce the flux of triosephosphate from chloroplasts; accumulation of phosphorylated intermediates indicates this but there is no evidence of impaired capacity for transport. Loss of SPS activity is thought to depend on C i rather than on RWC per se (Vassey et al 1991). In Phaseolus leaves in air, a decrease in RWC from 100 to 80% decreased g s, C i and SPS activity, the latter by more than 60% (Vassey & Sharkey 1989).…”

Section: Carbohydrate Metabolismmentioning

confidence: 99%

Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants

Lawlor

Cornic

2002

Plant Cell & Environment

1,597

964

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SUMMARYExperimental studies on CO 2 assimilation of mesophytic C3 plants in relation to relative water content (RWC) are discussed. Decreasing RWC slows the actual rate of photosynthetic CO 2 assimilation (A) and decreases the potential rate (A pot ). Generally, as RWC falls from c . 100 to c. 75%, the stomatal conductance (g s ) decreases, and with it A. However, there are two general types of relation of A pot to RWC, which are called Type 1 and Type 2. Type 1 has two main phases. As RWC decreases from 100 to c. 75%, A pot is unaffected, but decreasing stomatal conductance (g s ) results in smaller A, and lower CO 2 concentration inside the leaf (C i ) and in the chloroplast (C c ), the latter falling possibly to the compensation point. Down-regulation of electron transport occurs by energy quenching mechanisms, and changes in carbohydrate and nitrogen metabolism are considered acclimatory, caused by low C i and reversible by elevated CO 2 . Below 75% RWC, there is metabolic inhibition of A pot , inhibition of A then being partly (but progressively less) reversible by elevated CO 2 ; g s regulates A progressively less, and C i and CO 2 compensation point, Γ Γ Γ Γ rise. It is suggested that this is the true stress phase, where the decrease in A pot is caused by decreased ATP synthesis and a consequent decreased synthesis of RuBP. In the Type 2 response, A pot decreases progressively at RWC 100 to 75%, with A being progressively less restored to the unstressed value by elevated CO 2 . Decreased g s leads to a lower C i and C c but they probably do not reach compensation point: g s becomes progressively less important and metabolic limitations more important as RWC falls. The primary effect of low RWC on A pot is most probably caused by limited RuBP synthesis, as a result of decreased ATP synthesis, either through inhibition of Coupling Factor activity or amount due to increased ion concentration. Carbohydrate synthesis and accumulation decrease. Type 2 response is considered equivalent to Type 1 at RWC below c. 75%, with A pot inhibited by limited ATP and RuBP synthesis, respiratory metabolism dominates and C i and Γ Γ Γ Γ rise. The importance of inhibited ATP synthesis as a primary cause of decreasing A pot is discussed. Factors determining the Type 1 and Type 2 responses are unknown. Electron transport is maintained (but down-regulated) in Types 1 and 2 over a wide range of RWC, and a large reduced/oxidized adenylate ratio results. Metabolic imbalance results in amino acid accumulation and decreased and altered protein synthesis. These conditions profoundly affect cell functions and ultimately cause cell death. Type 1 and 2 responses may reflect differences in g s and in sensitivity of metabolism to decreasing RWC.

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“…Water stress can affect foliar SPS activity (Quick et al, 1989;Vassey et al, 1991). In the present experiments, RH was maintained to keep a similar vapor-pressure deficit in both chambers.…”

Section: Dlscusslonmentioning

confidence: 99%

“…SPS activity in mature leaves was reduced in plants subjected to water stress (Vassey and Sharkey, 1989;Vassey et al, 1991;Castrillo, 1992). This decrease in SPS activity was attributed to inhibition of photosynthesis as a result of stomatal closure (Vassey et al, 1991). However, pigeonpea and soybean plants exposed to water stress had increased SPS activity (Cheikh and Brenner, 1992;Keller and Ludlow, 1993).…”

mentioning

confidence: 99%

Effect of High Temperature on Plant Growth and Carbohydrate Metabolism in Potato

1995

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This study was undertaken to determine the role of sucrosemetabolizing enzymes in altered carbohydrate partitioning caused by heat stress. Potato (Solanum tuberosum L.) genotypes characterized as susceptible and tolerant to heat stress were grown at 19/ 17"C, and a subset was transferred to 31/29"C. Data were obtained for plant growth and photosynthesis. Enzyme activity was determined for sucrose-6-phosphate synthase (SPS) in mature leaves and for sucrose synthase, ADP-glucose pyrophosphorylase, and UDPglucose pyrophosphorylase in developing tubers of plants. High temperatures reduced growth of tubers more than of shoots. Photosynthetic rates were unaffected or increased slightly at the higher temperature. Heat stress increased accumulation of foliar sucrose and decreased starch accumulation in mature leaves but did not affect glucose. SPS activity increased significantly in mature leaves of plants subjected to high temperature. Changes in SPS activity were probably not due to altered enzyme kinetics. l h e activity of sucrose synthase and ADP-glucose pyrophosphorylase was reduced in tubers, albeit less quickly than leaf SPS activity. There was no interaction of temperature and genotype with regard to the enzymes examined; therefore, observed differences do not account for differences between genotypes in heat susceptibility.

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Water stress, carbon dioxide, and light effects on sucrose-phosphate synthase activity in Phaseolus vulgaris

Cited by 35 publications

References 30 publications

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants

Effect of High Temperature on Plant Growth and Carbohydrate Metabolism in Potato

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