Water‐Soluble Triarylboron Compound for ATP Imaging In Vivo Using Analyte‐Induced Finite Aggregation

Li, Xiaoyan; Guo, Xudong; Cao, Lixia; Xun, Zhiqing; Wang, Shuangqing; Li, Shayu; Li, Yang; Yang, Guoqiang

doi:10.1002/ange.201403918

Cited by 30 publications

(9 citation statements)

References 43 publications

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“…Recently, the imidazolium group has been reported to be an efficient recognition site for ATP, which can also discriminate between tri-, di-, and monophosphate groups. 34,35 This group has been identified as a candidate recognition structure for ATP sensing and imaging. Combining the advantages of CPs and this ATP recognition site might allow more efficient ATP sensing.…”

Section: ■ Introductionmentioning

confidence: 99%

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Cui

Yang

Yao

et al. 2016

ACS Appl. Mater. Interfaces

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Water-soluble conjugated polymers are attractive fluorescent materials for applications in chemical and biological sensing. The molecular wire effect of such polymers amplifies changes in the fluorescence signal, which can be used for detecting various analytes with high sensitivity. In this work, we report an efficient ratiometric fluorescent probe based on a water-soluble conjugated polymer that showed high sensitivity and selectivity toward adenosine 5'-triphosphate (ATP). The macromolecular probe consisted of a polyfluorene backbone doped with 5 mol % 1,4-dithienylbenzothiadiazole (DBT) modified by bis-imidazolium and oligo(ethylene glycol) moieties. Solutions of the polymer emitted purple fluorescence, which changed to red upon addition of ATP molecules. The addition of ATP caused the polymer to aggregate, which enhanced fluorescence resonance energy transfer efficiency from the fluorene segments to DBT units, leading to an increase in red emission. The ratio of the fluorescence at these different wavelengths (I/I) showed a strong dependence on the ATP concentration. PF-DBT-BIMEG also exhibited high selectivity for ATP sensing over other representative anions and discriminated it from adenosine 5'-diphosphate (ADP) and adenosine 5'-monophosphate (AMP). This can be explained by the much stronger electrostatic interactions between the polymer and ATP than the interactions between the polymer and ADP or AMP, as confirmed through molecular dynamics simulations.

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Section: ■ Introductionmentioning

confidence: 99%

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Cui

Yang

Yao

et al. 2016

ACS Appl. Mater. Interfaces

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“…Confocal fluorescence images showed the increase of cellular fluorescence with the prolongation of time after the incubation of QDs@TA‐PEG (Figure d). To verify the potential of the NCs for monitoring intracellular ATP level, HepG2 cells were cultured with the NCs in the presence of apyrase, which can induce the reduction of intracellular ATP contents by catalyzing the hydrolysis of ATP . Cellular uptake study indicated that cellular NCs contents were not obviously affected with apyrase treatment (Figure S11, Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Enhancing Antitumor Efficacy by Simultaneous ATP‐Responsive Chemodrug Release and Cancer Cell Sensitization Based on a Smart Nanoagent

Song

Guo

et al. 2018

Advanced Science

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The exploitation of smart nanoagents based drug delivery systems (DDSs) has proven to be a promising strategy for fighting cancers. Hitherto, such nanoagents still face challenges associated with their complicated synthesis, insufficient drug release in tumors, and low cancer cell chemosensitivity. Here, the engineering of an adenosine triphosphate (ATP)‐activatable nanoagent is demonstrated based on self‐assembled quantum dots‐phenolic nanoclusters to circumvent such challenges. The smart nanoagent constructed through a one‐step assembly not only has high drug loading and low cytotoxicity to normal cells, but also enables ATP‐activated disassembly and controlled drug delivery in cancer cells. Particularly, the nanoagent can induce cell ATP depletion and increase cell chemosensitivity for significantly enhanced cancer chemotherapy. Systematic in vitro and in vivo studies further reveal the capabilities of the nanoagent for intracellular ATP imaging, high tumor accumulation, and eventual body clearance. As a result, the presented multifunctional smart nanoagent shows enhanced antitumor efficacy by simultaneous ATP‐responsive chemodrug release and cancer cell sensitization. These findings offer new insights toward the design of smart nanoagents for improved cancer therapeutics.

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“…To do this, HeLa cells were initially treated with apyrase (1 U/ mL), an enzyme that could convert ATP into AMP. 23 The subsequent incubation of assembled nanoprobes showed much weaker fluorescence (Figure 5D,E), which was well supported by the population analysis using flow cytometry (Figure 5F). We further demonstrated that the decrease of activation fluorescence was not derived from the fewer nanoprobes uptake.…”

Section: Acs Sensorsmentioning

confidence: 99%

Assembly of DNA Probes into Superstructures for Dramatically Enhancing Enzymatic Stability and Signal-to-Background Ratio

Wang

Zhang

et al. 2018

ACS Sens.

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DNA fluorescent probes are versatile tools that are widely used for biological detection in tubes. Using DNA probes in living systems, however, represents a significant challenge because of the endogenous nuclease-induced DNA degradation and strong background fluorescence in complex biological environments. Here, we show that assembling DNA probes into core−satellite gold nanoparticle (AuNP) superstructures could unprecedentedly enhance enzymatic stability and reduce background interference. The embedded DNA probes are protected from interaction with nuclease, eliminating the enzymatic degradation. In the meantime, the AuNP superstructures show extremely high quenching efficiency (>98%) toward the embedded DNA probes, whose fluorescence can be instantly turned on by the target binding, resulting in high signal-to-background ratio. To demonstrate these distinct properties, we made use of the assembled nanoprobes to monitor the ATP levels under different stimuli in living cells. The assembly strategy leads to a new opportunity for accurately sensing targets in living systems.

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Water‐Soluble Triarylboron Compound for ATP Imaging In Vivo Using Analyte‐Induced Finite Aggregation

Cited by 30 publications

References 43 publications

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Aggregation-Induced Energy Transfer of Conjugated Polymer Materials for ATP Sensing

Enhancing Antitumor Efficacy by Simultaneous ATP‐Responsive Chemodrug Release and Cancer Cell Sensitization Based on a Smart Nanoagent

Assembly of DNA Probes into Superstructures for Dramatically Enhancing Enzymatic Stability and Signal-to-Background Ratio

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