Water-soluble polysaccharide obtained from Acorus calamus L. classically activates macrophages and stimulates Th1 response

Belska, N. V.; Guriev, A. M.; Данилец, М. Г.; Trophimova, E. S.; Uchasova, Evgenya; Ligatcheva, A. A.; Belousov, M. V.; Agaphonov, V. I.; Golovchenko, V.G.; Yusubov, M. S.; Belsky, Yu. P.

doi:10.1016/j.intimp.2010.05.005

Cited by 46 publications

(21 citation statements)

References 68 publications

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“…It also induced TNF-α secretion by human peripheral blood mononuclear cells, and reduced arginase activity, but did not affect IL-10 secretion by murine macrophages or human peripheral blood mononuclear cells. The IL-12 and NO-stimulating effects on murine macrophages were similar to that of LPS [35]. In this study we have shown that the TPS1-2 sub-fraction at 1.89 μg/mL gave similar phagocytosis effect as LPS (1 μg/mL).…”

Section: Resultssupporting

confidence: 54%

Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea

Wang

Wei

Liu

et al. 2014

IJMS

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Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity. The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively. Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2. A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-d-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively. The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells.

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Section: Resultssupporting

confidence: 54%

Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea

Wang

Wei

Liu

et al. 2014

IJMS

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“…Since NO, IFN-c, and TNF-a play a critical in the antimicrobial and tumoricidal activities of macrophages, great attention has been paid to developing drugs that modulate NO and TNF-a production (Poderoso et al, 1999). Many polysaccharides can activate macrophages by inducing the production of cytokines (Belska et al, 2010;Lee et al, 2010;Togola et al, 2008). Our study showed that AMP promoted NO, TNF-a, and IFN-c secretion, indicating its stimulatory effect in the host defense against microbial infection on activated macrophages.…”

Section: Discussionmentioning

confidence: 59%

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Chen

Zheng

2014

Pharmaceutical Biology

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Context: Atractylodes macrocephala Koidz is a traditional herb. Atractylodes macrocephalaon polysaccharides (AMP) have been found to enhance immunity and improve heart function. However, the mechanisms of the immunomodulatory effect have not been investigated. Objective: We examined whether AMP activated macrophages and explored the mechanisms of activation. Materials and methods: AMP was prepared and evaluated its immunomodulatory activity (25, 50, 100, and 200 lg/mL) by detecting the phagocytosis and the production of tumor necrosis factor-a (TNF-a), IFN-c, and nitric oxide (NO) in RAW264.7 macrophages. Furthermore, the role of nuclear factor-kB (NF-kB) pathway was examined in regulating TNF-a and NO production. Results: The phagocytosis of macrophages was enhanced by AMP in a dose-dependent manner and the maximal phagocytosis of macrophages occurred at concentrations of 100 and 200 lg/mL. NO, TNF-a, and IFN-c release was also found to be dose dependent by increasing concentrations of AMP and reached the peak at a concentration of 200 lg/mL. In addition, AMP induced inhibitor kappaB (IkB) degradation and the activation of NF-kB by p65 nuclear translocation, and then the activation of NF-kB in nucleus peaked at a concentration of 200 lg/ mL. Besides, NF-kB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) decreased AMP-induced NO and TNF-a production. Discussion and conclusion: These data suggest that AMP may modulate macrophage activities by stimulating NF-kB or activating NF-kB-dependent mechanisms.

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“…This fraction contains low molecular weight galacturonans (96.5% UA, Table 1), resulting from endo-polygalacturonase treatment of Et75A fraction and retained in 1 kDa dialysis membrane. As the homogalacturonan moiety of pectic polysaccharides has been reported as macrophage immunostimulator (Belska et al, 2010;Wang et al, 2013Wang et al, , 2014, it is possible that, when present in concentrations such as 200 g/mL, the homogalacturonan moieties of pectic polysaccharides from P. tridentatum dried inflorescences also contribute to macrophage NO production. Table 4 summarizes the relationship between the polysaccharide composition of the different fractions and their macrophage NO production.…”

Section: Tablementioning

confidence: 99%

In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

Martins

Simões

Ferreira

et al. 2017

Carbohydrate Polymers

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a b s t r a c tPterospartum tridentatum (L.) Willk. decoctions of dried inflorescences are used in Portugal due to their claimed beneficial properties for various health disorders. To disclose the potential contribution of its polysaccharides to health benefits, in this work, hot water extracts from P. tridentatum inflorescences were prepared and fractionated by ethanol precipitation and anion exchange chromatography. The fraction rich in acetylated galactomannans evidenced an increase in nitric oxide (NO) production by macrophages. This activity decreased 60-75% after saponification, confirming that acetylation is an important structural feature for this biological property. In addition, the treatment of pectic polysaccharides with endopolygalacturonase showed that type-I and type-II arabinogalactans, as well as low molecular weight galacturonans and xyloglucans, may also contribute to macrophage NO production. Thus, the polysaccharides present in P. tridentatum dried inflorescences may contribute to the health beneficial properties frequently attributed to the decoctions of this plant.

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Water-soluble polysaccharide obtained from Acorus calamus L. classically activates macrophages and stimulates Th1 response

Cited by 46 publications

References 68 publications

Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea

Characterization of Two Homogalacturonan Pectins with Immunomodulatory Activity from Green Tea

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

In vitro macrophage nitric oxide production by Pterospartum tridentatum (L.) Willk. inflorescence polysaccharides

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