Water soluble luminescent cyclometalated platinum(II) complex — A suitable probe for bio-imaging applications

Pasha, Sheik Saleem; Das, Pradip; Rath, Nigam P.; Bandyopadhyay, Debashree; Jana, Nikhil R.; Laskar, Inamur Rahaman

doi:10.1016/j.inoche.2016.03.017

Cited by 25 publications

(31 citation statements)

References 34 publications

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“…The bAgNPs (1) were tagged with the reported luminescent platinum (II) complex. 34 The resulting composite (2) was observed to emit green light under exposure to UV light (λ ext ∼ 365 nm) in suspended medium. As the luminescent platinum (II) complex emits green light in water solution, we presumed that the dye has adhered to the surface of NPs ( Figure S1a ).…”

Section: Resultsmentioning

confidence: 99%

“…Measurements were recorded at 25 °C ± 1 °C, in triplicates; each measurement was the average of 20 data sets acquired for 10 s each. 34 Thereafter, following confirmation of composite formation, AgNP internalization was confirmed by the exposure of platinum–AgNP composite on OS cells for 1 h and its subsequent visualization by a fluorescence microscope using FITC (λ ex/em 490/520) and DAPI (λ ex/em 372/456) filters.…”

Section: Materials and Methodsmentioning

confidence: 99%

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Biosynthesized Protein-Capped Silver Nanoparticles Induce ROS-Dependent Proapoptotic Signals and Prosurvival Autophagy in Cancer Cells

et al. 2017

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In recent years, the use of silver nanoparticles (AgNPs) in biomedical applications has shown an unprecedented boost along with simultaneous expansion of rapid, high-yielding, and sustainable AgNP synthesis methods that can deliver particles with well-defined characteristics. The present study demonstrates the potential of metal-tolerant soil fungal isolate Penicillium shearii AJP05 for the synthesis of protein-capped AgNPs. The particles were characterized using standard techniques, namely, UV–visible spectroscopy, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. The anticancer activity of the biosynthesized AgNPs was analyzed in two different cell types with varied origin, for example, epithelial (hepatoma) and mesenchymal (osteosarcoma). The biological NPs (bAgNPs) with fungal-derived outer protein coat were found to be more cytotoxic than bare bAgNPs or chemically synthesized AgNPs (cAgNPs). Elucidation of the molecular mechanism revealed that bAgNPs induce cytotoxicity through elevation of reactive oxygen species (ROS) levels and induction of apoptosis. Upregulation of autophagy and activation of JNK signaling were found to act as a prosurvival strategy upon bAgNP treatment, whereas ERK signaling served as a prodeath signal. Interestingly, inhibition of autophagy increased the production of ROS, resulting in enhanced cell death. Finally, bAgNPs were also found to sensitize cells with acquired resistance to cisplatin, providing valuable insights into the therapeutic potential of bAgNPs. To the best of our knowledge, this is the first study that provides a holistic idea about the molecular mechanisms behind the cytotoxic activity of protein-capped AgNPs synthesized using a metal-tolerant soil fungus.

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Section: Resultsmentioning

confidence: 99%

Section: Materials and Methodsmentioning

confidence: 99%

Biosynthesized Protein-Capped Silver Nanoparticles Induce ROS-Dependent Proapoptotic Signals and Prosurvival Autophagy in Cancer Cells

et al. 2017

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“…While a vast number of Pt(II) complexes reported in the literature are luminescent, only a small group are fully soluble in H 2 O. 11 Also, the photoluminescence of such phosphorescent complexes can be quenched by triplet molecular dioxygen ( 3 O 2 ) dissolved in the medium, which limits their applicability in bioimaging, as their τ and Φ L are severely reduced along with the production of cytotoxic singlet oxygen ( 1 O 2 ). 12−14 We have previously observed that metal−metal interactions between Pt(II) complexes self-assembling into phosphorescent fibers, gels, and polymers result in supramolecular arrays with reduced diffusion-controlled collisional quenching by 3 O 2 .…”

Section: ■ Introductionmentioning

confidence: 99%

Intermolecular Interactions and Self-Assembly in Pt(II) Complex–Nanoclay Hybrids as Luminescent Reporters for Spectrally Resolved PLIM

et al. 2021

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Efficient shielding of phosphorescent transition metal complexes against diffusion-controlled collisional quenching by triplet molecular dioxygen as well as reduction of microenvironment-related radiationless deactivation pathways is crucial for their applications in bioimaging and optoelectronics. In this report, we present a straightforward yet efficient approach to safeguard emissive triplet states from external influences by adsorbing phosphorescent Pt(II) complexes onto a layered nanoclay, namely Laponite. These hybrids facilitate the dispersion of otherwise insoluble transition metal complexes in aqueous media while shielding them from physical quenching. Self-assembly of the nanoclay and intermolecular stacking between molecules adsorbed at different nanodisc units are mirrored in the photophysical, colloidal, and morphological properties of the hybrids, which were herein characterized by steady-state and time-resolved photoluminescence spectroscopy, dynamic light scattering, and atomic force microscopy. We also show that the hybrids are noncytotoxic and can be exploited as luminescent reporters in spectrally resolved phosphorescence lifetime imaging implemented by confocal optical microscopy.

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“…Other examples of Ir(III) complexes have been reported to sense changes in the mitochondrial environment, either for hypoxic conditions [ 257 ] or labile Zn 2+ ions [ 258 ]. There are also examples of Pt(II) [ 259 , 260 ] and Ru(II) [ 261 , 262 ] metal ion complexes for cellular imaging; however, these are less favoured options when compared to the Re(II) and Ir(III) alternatives, presumably owing to their comparatively weaker photophysical properties. Moreover, transition metal complexes have been used for sensing purposes too, including endogenous nitric oxide [ 263 ] and thiol [ 264 ] sensing using Ru(II) complexes.…”

Section: Imaging Agents Used For Fluorescence Microscopymentioning

confidence: 99%

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

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Ung

Bader

et al. 2021

Cells

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Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

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Water soluble luminescent cyclometalated platinum(II) complex — A suitable probe for bio-imaging applications

Cited by 25 publications

References 34 publications

Biosynthesized Protein-Capped Silver Nanoparticles Induce ROS-Dependent Proapoptotic Signals and Prosurvival Autophagy in Cancer Cells

Biosynthesized Protein-Capped Silver Nanoparticles Induce ROS-Dependent Proapoptotic Signals and Prosurvival Autophagy in Cancer Cells

Intermolecular Interactions and Self-Assembly in Pt(II) Complex–Nanoclay Hybrids as Luminescent Reporters for Spectrally Resolved PLIM

Fluorescence Microscopy—An Outline of Hardware, Biological Handling, and Fluorophore Considerations

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