Water soluble levan polysaccharide biopolymer electrospun fibers

Manandhar, Sandeep; Vidhate, Shailesh; D’Souza, Nandika Anne

doi:10.1016/j.carbpol.2009.06.023

Cited by 42 publications

(18 citation statements)

References 6 publications

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“…It is interesting to note that levan is a water soluble polysaccharide [66] with an unusually low viscosity [67]. At concentrations where typical polysaccharides display adhesive behavior by forming gels or pastes, levan solutions remain fluid [66], which would suggest that levan by itself cannot form a strong and sticky network of a typical biofilm.…”

Section: Discussionmentioning

confidence: 99%

“…At concentrations where typical polysaccharides display adhesive behavior by forming gels or pastes, levan solutions remain fluid [66], which would suggest that levan by itself cannot form a strong and sticky network of a typical biofilm. This is in agreement with our observation that significant amounts of water-soluble non-sticky levan were detected in the SYM spent medium below the biofilms.…”

Section: Discussionmentioning

confidence: 99%

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Exopolymer Diversity and the Role of Levan in Bacillus subtilis Biofilms

et al. 2013

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Exopolymeric substances (EPS) are important for biofilm formation and their chemical composition may influence biofilm properties. To explore these relationships the chemical composition of EPS from Bacillus subtilis NCIB 3610 biofilms grown in sucrose-rich (SYM) and sucrose-poor (MSgg and Czapek) media was studied. We observed marked differences in composition of EPS polymers isolated from all three biofilms or from spent media below the biofilms. The polysaccharide levan dominated the EPS of SYM grown biofilms, while EPS from biofilms grown in sucrose-poor media contained significant amounts of proteins and DNA in addition to polysaccharides. The EPS polymers differed also in size with very large polymers (Mw>2000 kDa) found only in biofilms, while small polymers (Mw<200 kD) dominated in the EPS isolated from spent media. Biofilms of the eps knockout were significantly thinner than those of the tasA knockout in all media. The biofilm defective phenotypes of tasA and eps mutants were, however, partially compensated in the sucrose-rich SYM medium. Sucrose supplementation of Czapek and MSgg media increased the thickness and stability of biofilms compared to non-supplemented controls. Since sucrose is essential for synthesis of levan and the presence of levan was confirmed in all biofilms grown in media containing sucrose, this study for the first time shows that levan, although not essential for biofilm formation, can be a structural and possibly stabilizing component of B. subtilis floating biofilms. In addition, we propose that this polysaccharide, when incorporated into the biofilm EPS, may also serve as a nutritional reserve.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exopolymer Diversity and the Role of Levan in Bacillus subtilis Biofilms

et al. 2013

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“…These results completely agree with the literature in that an increase in the feeding rate can increase the diameter of nanofibers. Bigger Taylor cone has been produced at the needle in a high injection rate and attained finer nanofibers [44]. However, a very low injection rate could not supply a sufficient amount of solution at the tip of the needle because an excessive increase in the flow rate can result in nanofibers without sufficient solvent evaporation and with flattened, beaded web-like appearance [45].…”

Section: Effect Of Parameters On Nanofiber Diametermentioning

confidence: 99%

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

et al. 2020

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The kefiran/polyvinyl alcohol and polyvinyl pyrrolidone nanofibers were effectively fabricated for the first time using electrospinning of poly(vinyl alcohol), poly(vinyl pyrrolidone) and kefiran blend solution. The effect of solution parameters, such as kefiran concentration and poly(vinyl alcohol)/poly(vinyl pyrrolidone) mixing ratio, were considered. The parameters' effects, such as voltage, nozzle-to-collector distance and feeding rate on nanofibers morphology, were also checked by the scanning electron microscopy images. After changing the parameters on different steps, designed by the chemo metrics software, the finest nanofibers were produced in the following conditions: 2% kefiran concentration, 30/70 kefiran/polymer mixing ratio, 12 kV voltage, 200 mm needle-to-collector distance and 0.5 (mL/h) polymer injection rate. The nanofibers produced in these conditions were uniform without knots or adhesion in the lowest diameter of 156 nm. Kefiran concentration and kefiran/polymer mixing ratio were found as the most effective parameter in the morphology and diameter size of the nanofibers. The attenuated total reflectance (ATR-Ft-IR), atomic force spectroscopy, differential scanning calorimeters and thermal gravimetric analysis were used to investigate molecular structures, threedimensional morphology and heating properties of the nanofibers, respectively.

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“…Higher concentrations of the polymer enable the formation of chain entanglements required to maintain the jet strength to produce the nanofibers. Smaller fibers were produced with increasing voltage, decreasing flow rate, and decreasing distance between collector plate and needle …”

Section: Synthetic Polymersmentioning

confidence: 99%

Green Processing of Nanofibers for Regenerative Medicine

Krishnan

Sundarrajan

Ramakrishna

2012

Macro Materials & Eng

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Electrospinning of polymer scaffolds is mostly carried out using organic solvents, but the drawbacks are: solvent costs, environmental hazards, and presence of traces of solvent impurities. The use of water‐soluble polymers (WSPs), water or aqueous solutions as an electrospinning medium (green processing) is a very attractive method to avoid such issues. However, a few WSP such as polyelectrolytes are not spinnable as such, but have been electrospun by addition of WSPs, additives, and salts. This paper covers solution properties, polyelectrolyte nanofibrous scaffolds (polysaccharides, biopolymers, etc.), fabrication through green processing, and their regenerative medical applications such as wound dressing, drug delivery, and tissue engineering. This is the first review to cover the above issues, the drawbacks of current methods, and future challenges.

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Water soluble levan polysaccharide biopolymer electrospun fibers

Cited by 42 publications

References 6 publications

Exopolymer Diversity and the Role of Levan in Bacillus subtilis Biofilms

Exopolymer Diversity and the Role of Levan in Bacillus subtilis Biofilms

Kefiran/poly(vinyl alcohol)/poly(vinyl pyrrolidone) composite nanofibers: fabrication, characterization and consideration of effective parameters in electrospinning

Green Processing of Nanofibers for Regenerative Medicine

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