Water-soluble germanium nanoparticles cause necrotic cell death and the damage can be attenuated by blocking the transduction of necrotic signaling pathway

Yu, Hongbin; Huang, Chin Ping; Tsai, Jia Shiuan; Shen, Mo Yuan; Li, Yaw-Kuen; Lin, Lih-Yuan

doi:10.1016/j.toxlet.2011.09.018

Cited by 30 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A comprehensive series of state-of-the-art proliferative, cytotoxic, cell viability and behavioural studies were performed as the cells were exposed to bio- relevant concentrations of the WDWs. Previous work with GeNPs used concentration ranges spanning 0–5 µM which we used as the basis for this work [23]. Two well established cell lines; MCF-7 (human breast carcinoma cells) and L929 (murine fibroblasts) were employed for this study as their behaviour has been well studied in our laboratory [35]–[37].…”

Section: Resultsmentioning

confidence: 99%

“…However, it is documented that Germanium nanoparticles (GeNPs) of 4.2±1.2 nm display cytotoxicity in CHO cells [23]. The GeNPs at low concentrations (<5 µM) promote necrotic cell death.…”

Section: Introductionmentioning

confidence: 99%

“…The GeNPs at low concentrations (<5 µM) promote necrotic cell death. In order to make GeNPs water dispersible, ligands such as alkyamines are employed on their surface [23]. However, as seen with silver nanoparticles [24], the GeNPs may only be acting as carriers of the toxic ligand.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Promoting Cell Proliferation Using Water Dispersible Germanium Nanowires

et al. 2014

View full text Add to dashboard Cite

Group IV Nanowires have strong potential for several biomedical applications. However, to date their use remains limited because many are synthesised using heavy metal seeds and functionalised using organic ligands to make the materials water dispersible. This can result in unpredicted toxic side effects for mammalian cells cultured on the wires. Here, we describe an approach to make seedless and ligand free Germanium nanowires water dispersible using glutamic acid, a natural occurring amino acid that alleviates the environmental and health hazards associated with traditional functionalisation materials. We analysed the treated material extensively using Transmission electron microscopy (TEM), High resolution-TEM, and scanning electron microscope (SEM). Using a series of state of the art biochemical and morphological assays, together with a series of complimentary and synergistic cellular and molecular approaches, we show that the water dispersible germanium nanowires are non-toxic and are biocompatible. We monitored the behaviour of the cells growing on the treated germanium nanowires using a real time impedance based platform (xCELLigence) which revealed that the treated germanium nanowires promote cell adhesion and cell proliferation which we believe is as a result of the presence of an etched surface giving rise to a collagen like structure and an oxide layer. Furthermore this study is the first to evaluate the associated effect of Germanium nanowires on mammalian cells. Our studies highlight the potential use of water dispersible Germanium Nanowires in biological platforms that encourage anchorage-dependent cell growth.

show abstract

Section: Resultsmentioning

confidence: 99%

“…However, it is documented that Germanium nanoparticles (GeNPs) of 4.2±1.2 nm display cytotoxicity in CHO cells [23]. The GeNPs at low concentrations (<5 µM) promote necrotic cell death.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Promoting Cell Proliferation Using Water Dispersible Germanium Nanowires

et al. 2014

View full text Add to dashboard Cite

show abstract

“…2010 Hussain and co-workers 66 reported apoptosis of cells after NP exposure and they proved that there are two different pathways for apoptosis activated by two different kinds of NPs. One year later Ma et al 67 reported investigations of water-soluble germanium NPs, which caused necrotic cell death. They found that the damage can be attenuated by blocking the transduction of necrotic signaling pathway which proofed that necrosis not apoptosis was responsible for cell death in their experimental system.…”

Section: B Np Effects In Vitromentioning

confidence: 99%

Current systems biology approaches in hazard assessment of nanoparticles

Ehrhart¹,

Evelo²,

Willighagen³

2015

Preprint

View full text Add to dashboard Cite

The amount of nanoparticles (NPs) in human environment is increasing. The main sources are the increased introduction in consumer products and air pollution (diesel exhaust). It is meanwhile common knowledge that NPs behave differently as bulk material because of their nano-size. This leads in general to a higher reactivity and some other changed properties, e.g. solubility, surface potential, conductivity, and, to different effects on biological systems. The main impacts of NPs on a cellular and organism level are meanwhile well known: release of toxic ions, increased oxidative stress, and inflammation. Beside these, there is increasing evidence that NPs, especially in low dose/long exposure scenarios, affect biological systems in a broader way, interact with drugs, and exacerbate the effects of diseases. To investigate these effects systems biology approaches are the method of choice. This review summarizes the state of the art of nanoparticle effects on cells and organisms and demonstrate the add value of systems biology investigations to NP hazard assessment.Abbreviations: nanoparticle (NP), carbon nanotube (CNT), single walled carbon nanotube (SWCNT), multi walled carbon nanotube (MWCNT), ADME (adsorption, distribution, metabolization, excretion), PAMAM (poly(amidoamine)), Particles of the same material source but small differences in size, surface (coating), charge or solvent components may exhibit different properties 2 . Systems biology is a holistic approach to model complex biological systems using mathematical and computer science methods. Basis of systems biology are the single components and entities of a biological system: genes, transcripts, proteins, and metabolites (see Figure 1). A good understanding and a description of the single entities and how they are interacting within a biological system is crucial for systems biology approaches. Based on this, time or incident dependent changes, e.g. exposure of NPs, in the system can be investigated. The interactions and relations between the entities are commonly described in biological pathways and networks. The state-of-art measuring technologies like genome sequencing and high throughput measurements enables the researchers to study the overall behavior and phenotype of a biological system in a defined setting. So, besides "listing the parts of an airplane" the scientific community proceeds to study the system as a whole 3 . This thorough understanding finally allows prediction of behavior using computational models. Figure 1 Scheme of systems biology. Biologic entities like genes, proteins or metabolites are connected by interactions forming the structure of the system. This structure alters with time and reacts to incidents demonstrating systems dynamics and control mechanisms.In this review we focus on the actual status of hazard assessment of NPs and the added value of using systems biology tools and methods in the assessment. We excluded NPs for therapeutical use because cost-benefit analysis for drugs is differently evaluated. NP toxicologyNP...

show abstract

“…Moreover, it should not be underestimated that the induction of apoptosis in cell culture is inevitably followed by secondary necrosis, and this could lead to a misinterpretation of results. However, a recent study demonstrated that water-soluble germanium nanoparticles with allylamine-conjugated surfaces (4 nm) induce necrotic cell death that is not inhibited by necrostatin-1 in Chinese hamster ovary cells [130]. Although the mechanisms of ligand and surface chemistry, surface charge, and crystallinity-based toxicity are complex, studies are beginning to elucidate certain surface functional groups and properties that can effectively alter biological responses.…”

Section: Nanomaterials and Necrosismentioning

confidence: 99%

Nanomaterials Toxicity and Cell Death Modalities

Stefano

Carnuccio

Maiuri

2012

Journal of Drug Delivery

106

View full text Add to dashboard Cite

In the last decade, the nanotechnology advancement has developed a plethora of novel and intriguing nanomaterial application in many sectors, including research and medicine. However, many risks have been highlighted in their use, particularly related to their unexpected toxicity in vitro and in vivo experimental models. This paper proposes an overview concerning the cell death modalities induced by the major nanomaterials.

show abstract

Water-soluble germanium nanoparticles cause necrotic cell death and the damage can be attenuated by blocking the transduction of necrotic signaling pathway

Cited by 30 publications

References 39 publications

Promoting Cell Proliferation Using Water Dispersible Germanium Nanowires

Promoting Cell Proliferation Using Water Dispersible Germanium Nanowires

Current systems biology approaches in hazard assessment of nanoparticles

Nanomaterials Toxicity and Cell Death Modalities

Contact Info

Product

Resources

About