Water-soluble cranberry extract inhibits Vibrio cholerae biofilm formation possibly through modulating the second messenger 3’, 5’ - Cyclic diguanylate level

Pederson, Daniel B.; Dong, Yuqing; Blue, Levi B.; Smith, Sara V.; Cao, Min

doi:10.1371/journal.pone.0207056

Cited by 17 publications

(12 citation statements)

References 67 publications

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“…Previous studies have confirmed that phenolic compounds from plants, such as eugenol, tea polyphenols, and thymol, can inhibit the production of virulence-associated factors and biofilm formation in P. aeruginosa ( Yin et al, 2015 ; Walsh et al, 2019 ; Antunes et al, 2021 ). Scanning electron microscopy results further demonstrated that paeonol decreased biofilm formation against P. aeruginosa , possibly interfered with the subsequent steps of surface adhesion or biofilm formation ( Pederson et al, 2018 ; Liu et al, 2019 ). Additionally, the motility of P. aeruginosa is a complex process that is co-regulated by the las and rhl systems to promote the formation and spread of biofilms ( Glessner et al, 1999 ).…”

Section: Discussionmentioning

confidence: 86%

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

et al. 2021

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With the prevalence of multidrug-resistant bacteria and clinical -acquired pathogenic infections, the development of quorum-sensing (QS) interfering agents is one of the most potential strategies to combat bacterial infections and antibiotic resistance. Chinese herbal medicines constitute a valuable bank of resources for the identification of QS inhibitors. Accordingly, in this research, some compounds were tested for QS inhibition using indicator strains. Paeonol is a phenolic compound, which can effectively reduce the production of violacein without affecting its growth in Chromobacterium violaceum ATCC 12472, indicating its excellent anti-QS activity. This study assessed the anti-biofilm activity of paeonol against Gram-negative pathogens and investigated the effect of paeonol on QS-regulated virulence factors in Pseudomonas aeruginosa. A Caenorhabditis elegans infection model was used to explore the anti-infection ability of paeonol in vivo. Paeonol exhibited an effective anti-biofilm activity against Gram-negative bacteria. The ability of paeonol to interfere with the AHL-mediated quorum sensing systems of P. aeruginosa was determined, found that it could attenuate biofilm formation, and synthesis of pyocyanin, protease, elastase, motility, and AHL signaling molecule in a concentration- and time-dependent manner. Moreover, paeonol could significantly downregulate the transcription level of the QS-related genes of P. aeruginosa including lasI/R, rhlI/R, pqs/mvfR, as well as mediated its virulence factors, lasA, lasB, rhlA, rhlC, phzA, phzM, phzH, and phzS. In vivo studies revealed that paeonol could reduce the pathogenicity of P. aeruginosa and enhance the survival rate of C. elegans, showing a moderate protective effect on C. elegans. Collectively, these findings suggest that paeonol attenuates bacterial virulence and infection of P. aeruginosa and that further research elucidating the anti-QS mechanism of this compound in vivo is warranted.

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Section: Discussionmentioning

confidence: 86%

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

et al. 2021

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“…Eradication of biofilms is a serious global health concern in controlling bacterial infections because they cause microbial resistance to many antibiotics. 27 Although non-antibiotic therapeutic strategies do not develop antimicrobial resistance, to be effective, they should be able to penetrate the biofilm structure or inhibit biofilm formation by bacterial population. For the first time in this study, the antibiofilm activity of MSCs CM and MSCs CM-CS NPs against MDR V. cholerae strains was assessed.…”

Section: Discussionmentioning

confidence: 99%

<p>Evaluation of the Antimicrobial and Antibiofilm Effect of Chitosan Nanoparticles as Carrier for Supernatant of Mesenchymal Stem Cells on Multidrug-Resistant <em>Vibrio cholerae</em></p>

Saberpour¹,

Bakhshi²,

Peerayeh³

2020

IDR

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Aim The aim of the present study was to evaluate the in vitro antimicrobial and antibiofilm activity of chitosan nanoparticles (CS NPs) incorporated with mesenchymal stem cells-derived conditioned media (MSCs CM) on MDR Vibrio cholerae strains. Materials and Methods Chitosan NPs were prepared and characterized by dynamic light scattering (DLS), scanning electron microscope (SEM) and zeta potential measurement. MSCs CM were prepared and entrapped into MSCs CM-CS NPs composite and its release efficiency was measured. Antibacterial efficacy of nano structures was determined by disk diffusion and broth microdilution methods. Antibiofilm activity was assessed by crystal violet assay. Results BM-MSCs were characterized to be negative for CD34 and CD45 markers, positive for CD73 and CD44 markers, and able to differentiate into osteoblast and adipocyte cells. The mean particle size of 96.6% of chitosan NPs was 414.9 nm with a suitable zeta potential and SEM morphology. Entrapment efficiency of MSCs CM-CS NPs was 76.9%. Unstimulated MSCs CM-CS NPs composite as a novel and proficient therapeutic nanostructure against MDR V. cholerae strains showed the synergistic activity of the two components of MSCs CM and CS NPs, leading to greater bacterial killing compared to control groups. MSCs CM more efficiently inhibited biofilm formation, although MSCs CM-CS NPs was also appeared to be effective in inhibiting biofilm formation compared to CS NPs and control group. Conclusion The designed nanodrug composite showed the best release in conditions mimicking the physiological conditions of the intestinal lumen. Given the fact that no overuse or genetic event would cause the emergence of antimicrobial resistance against the MSCs CM-CS NPs nanodrug, it could be considered as a promising alternative for the treatment of MDR V. cholerae infections in clinical settings.

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“…The prevention of biofilm formation is preferred as a treatment option because extracellular polysaccharides make biofilms difficult to chemically remove from surfaces and can protect bacteria from antibiotics and other harmful environmental conditions [216][217][218]228]. In this regard, cranberry materials have been investigated for their ability to affect biofilm formation by a number of potential pathogens, and a range of effects and activities have been observed for different organisms and test environments (e.g., urine vs. saliva vs. juice) [63,[229][230][231][232][233][234]. This range of responses by different organisms may lead to either beneficial or harmful effects on overall human health, depending on both the bacteria present and the host environmental variables involved.…”

Section: Effects On Biofilm Formation and Bacterial Aggregationmentioning

confidence: 99%

Oligosaccharides and Complex Carbohydrates: A New Paradigm for Cranberry Bioactivity

Coleman

Ferreira

2020

Molecules

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Cranberry is a well-known functional food, but the compounds directly responsible for many of its reported health benefits remain unidentified. Complex carbohydrates, specifically xyloglucan and pectic oligosaccharides, are the newest recognized class of biologically active compounds identified in cranberry materials. Cranberry oligosaccharides have shown similar biological properties as other dietary oligosaccharides, including effects on bacterial adhesion, biofilm formation, and microbial growth. Immunomodulatory and anti-inflammatory activity has also been observed. Oligosaccharides may therefore be significant contributors to many of the health benefits associated with cranberry products. Soluble oligosaccharides are present at relatively high concentrations (~20% w/w or greater) in many cranberry materials, and yet their possible contributions to biological activity have remained unrecognized. This is partly due to the inherent difficulty of detecting these compounds without intentionally seeking them. Inconsistencies in product descriptions and terminology have led to additional confusion regarding cranberry product composition and the possible presence of oligosaccharides. This review will present our current understanding of cranberry oligosaccharides and will discuss their occurrence, structures, ADME, biological properties, and possible prebiotic effects for both gut and urinary tract microbiota. Our hope is that future investigators will consider these compounds as possible significant contributors to the observed biological effects of cranberry.

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Water-soluble cranberry extract inhibits Vibrio cholerae biofilm formation possibly through modulating the second messenger 3’, 5’ - Cyclic diguanylate level

Cited by 17 publications

References 67 publications

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

Paeonol Attenuates Quorum-Sensing Regulated Virulence and Biofilm Formation in Pseudomonas aeruginosa

<p>Evaluation of the Antimicrobial and Antibiofilm Effect of Chitosan Nanoparticles as Carrier for Supernatant of Mesenchymal Stem Cells on Multidrug-Resistant <em>Vibrio cholerae</em></p>

Oligosaccharides and Complex Carbohydrates: A New Paradigm for Cranberry Bioactivity

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