Water-soluble carotenoid proteins of cyanobacteria

Kerfeld, Cheryl A.

doi:10.1016/j.abb.2004.03.018

Cited by 72 publications

(64 citation statements)

References 46 publications

(72 reference statements)

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“…Another protective mechanism against enhanced UV-B is the biosynthesis and accumulation of sunscreen compounds like the yellow-brown lipid-soluble scytonemin that occurs predominantly in the extracellular sheaths of cyanobacteria playing an eminent role in preventing UVinduced photodamage (Garcia-Pichel et al 1992). Caroteniods, which are concentrated mainly in thylakoid membrane, cell membranes, and cell walls of cyanobacteria, also protect cells from lethal eVect of photooxidation by singlet oxygen (Edge et al 1997;Kerfeld 2004). Other important compounds for photoprotection are the mycosporine-like amino acids (MAAs) that have been found in many cyanobacterial species (Garcia-Pichel and Castenholz 1993;Ehling-Schulz et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Isolate-specific effects of ultraviolet radiation on photosynthesis, growth and mycosporine-like amino acids in the microbial mat-forming cyanobacterium Microcoleus chthonoplastes

Pattanaik

Roleda

Schumann

et al. 2007

Planta

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Microcoleus chthonoplastes constitutes one of the dominant microorganisms in intertidal microbial mat communities. In the laboratory, the eVects of repeated daily exposure to ultraviolet radiation (16:8 light:dark cycle) was investigated in unicyanobacterial cultures isolated from three diVerent localities (Baltic Sea = WW6; North Sea = STO and Brittany = BRE). Photosynthesis and growth were measured in time series (12-15 days) while UV-absorbing mycosporine-like amino acids (MAAs) and cellular integrity were determined after 12 and 3 days exposure to three radiation treatments [PAR (22 mol photon m ¡2 s ¡1 ) = P; PAR + UV-A (8 W m ¡2

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Section: Introductionmentioning

confidence: 99%

Isolate-specific effects of ultraviolet radiation on photosynthesis, growth and mycosporine-like amino acids in the microbial mat-forming cyanobacterium Microcoleus chthonoplastes

Pattanaik

Roleda

Schumann

et al. 2007

Planta

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“…In addition to the ocp gene, the genomes of several cyanobacterial strains contain one to four copies of genes encoding only the N-terminal domain of OCP (Kerfeld, 2004b;Kerfeld and Kirilovsky, 2013). The function of these genes, named rcp genes, is unknown.…”

Section: Ocp Quenches 1 O 2 More Efficiently Than Red Carotenoid Protmentioning

confidence: 99%

The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher

et al. 2014

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Cyanobacteria have developed a photoprotective mechanism that decreases the energy arriving at the photosynthetic reaction centers under high-light conditions. The photoactive orange carotenoid protein (OCP) is essential in this mechanism as a light sensor and energy quencher. When OCP is photoactivated by strong blue-green light, it is able to dissipate excess energy as heat by interacting with phycobilisomes. As a consequence, charge separation and recombination leading to the formation of singlet oxygen diminishes. Here, we demonstrate that OCP has another essential role. We observed that OCP also protects Synechocystis cells from strong orange-red light, a condition in which OCP is not photoactivated. We first showed that this photoprotection is related to a decrease of singlet oxygen concentration due to OCP action. Then, we demonstrated that, in vitro, OCP is a very good singlet oxygen quencher. By contrast, another carotenoid protein having a high similarity with the N-terminal domain of OCP is not more efficient as a singlet oxygen quencher than a protein without carotenoid. Although OCP is a soluble protein, it is able to quench the singlet oxygen generated in the thylakoid membranes. Thus, OCP has dual and complementary photoprotective functions as an energy quencher and a singlet oxygen quencher.

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“…The OCP, a 35 kDa protein that contains a single noncovalently bound carotenoid (15)(16)(17)(18), is the product of the slr1963 gene in Synechocystis PCC 6803 (16). Highly conserved homologs of the OCP are found in most of the cyanobacterial genomes.…”

mentioning

confidence: 99%

A photoactive carotenoid protein acting as light intensity sensor

Wilson

Punginelli

Gall

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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321

431

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Intense sunlight is dangerous for photosynthetic organisms. Cyanobacteria, like plants, protect themselves from light-induced stress by dissipating excess absorbed energy as heat. Recently, it was discovered that a soluble orange carotenoid protein, the OCP, is essential for this photoprotective mechanism. Here we show that the OCP is also a member of the family of photoactive proteins; it is a unique example of a photoactive protein containing a carotenoid as the photoresponsive chromophore. Upon illumination with blue-green light, the OCP undergoes a reversible transformation from its dark stable orange form to a red ''active'' form. The red form is essential for the induction of the photoprotective mechanism. The illumination induces structural changes affecting both the carotenoid and the protein. Thus, the OCP is a photoactive protein that senses light intensity and triggers photoprotection.cyanobacteria ͉ nonphotochemical quenching ͉ photoprotection ͉ phycobilisome

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Water-soluble carotenoid proteins of cyanobacteria

Cited by 72 publications

References 46 publications

Isolate-specific effects of ultraviolet radiation on photosynthesis, growth and mycosporine-like amino acids in the microbial mat-forming cyanobacterium Microcoleus chthonoplastes

Isolate-specific effects of ultraviolet radiation on photosynthesis, growth and mycosporine-like amino acids in the microbial mat-forming cyanobacterium Microcoleus chthonoplastes

The Cyanobacterial Photoactive Orange Carotenoid Protein Is an Excellent Singlet Oxygen Quencher

A photoactive carotenoid protein acting as light intensity sensor

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