In cut roses (Rosa hybrida L.), the vase life of ethylene-sensitive cultivars (SENS) is determined by ethylene, and that of ethylene-insensitive cultivars (INSENS) is closely related to water relations. This variation in senescence among rose cultivars makes it difficult to prolong the vase life of cut roses after harvest. In this study, we investigated the combination effect of germicide and ethylene inhibition on the postharvest characteristics of cut rose 'Matador' and 'Dolcetto' cultivars. Cut roses were treated with three preservative solutions: sodium hypochlorite (Cl), sodium hypochlorite + aminoisobutyric acid (ClA), and sodium hypochlorite + aminoisobutyric acid + 1-methylcyclopropene (ClAM), subsequently exposed to ethylene. We found that all treatments extended the vase life and improved the postharvest quality in the both rose cultivars. Among these, ClAM was the most effective treatment solution, significantly extending the vase life by 4.3 days in 'Matador' (SENS) and 4.7 days in 'Dolcetto' (INSENS) compared with control flowers. ClAM also effectively inhibited bacterial growth in the vase, enhanced solution uptake, and maintained the initial fresh weight and a positive water balance for longer periods. In addition, we found that ClAM strongly inhibited ethylene-induced petal senescence by suppressing the transcript levels of RhACS2 and RhACO1 in the both rose cultivars. These results suggest that ClAM can be used in a wide range of rose cultivars to prolong the longevity of cut roses due to its combination effects with inhibition of ethylene damage and improvement of water absorption.