Water permeability is a measure of severity in acute appendicitis

Pini, Nicola; Pfeifle, Viktoria A.; Kym, Urs; Keck, Simone; Galati, Virginie; Holland‐Cunz, Stefan; Gros, Stephanie J.

doi:10.1080/14756366.2017.1347167

Cited by 3 publications

(10 citation statements)

References 28 publications

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“…Similar results were obtained by Pini et al (2017), who studied the influence of appendicitis on the distribution of AQP1 in the wall of the appendix. In that study, AQP1 was observed in the enteric ganglia in cells, which were not immunoreactive to panneronal marker -microtubule associated protein 2 (MAP-2), which suggests that (as in the oesophagus) AQP1 is present in glial cells, not in neurons.…”

Section: Humanssupporting

confidence: 85%

“…In that study, AQP1 was observed in the enteric ganglia in cells, which were not immunoreactive to panneronal marker -microtubule associated protein 2 (MAP-2), which suggests that (as in the oesophagus) AQP1 is present in glial cells, not in neurons. A higher number of AQP1-positive cells were noted in the myenteric ganglia than the submucous ganglia (Pini et al, 2017). In the same study, AQP1positive nerve fibres were observed in the neuronal ganglia in the wall of the appendix (Pini et al, 2017).…”

Section: Humanssupporting

confidence: 52%

“…A higher number of AQP1-positive cells were noted in the myenteric ganglia than the submucous ganglia (Pini et al, 2017). In the same study, AQP1positive nerve fibres were observed in the neuronal ganglia in the wall of the appendix (Pini et al, 2017). These nerves were located near neurons immunoreactive to MAP-2 and surrounding them.…”

Section: Humansmentioning

confidence: 54%

“…These nerves were located near neurons immunoreactive to MAP-2 and surrounding them. Moreover, Pini et al (2017) reported that appendicitis causes an increase in the number of intramural ganglia, in which AQP1 -positive cells and nerve fibres were noted.…”

Section: Humansmentioning

confidence: 99%

“…Gao et al, 2006 AQP1 in intraganglionic glial cells and nerve fibres in the appendix. Pini et al, 2017 AQP4 in mucosal enteric glial cells in the colon. Jiang et al, 2014 RAT AQP1 in neuronal cells and nerve fibres in the ileum.…”

Section: Possible Functions Of Aqps In the Nervous Structures Supplyimentioning

confidence: 99%

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Aquaporins in the nervous structures supplying the digestive organs – a review

Gonkowski

2021

Annals of Animal Science

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Aquaporins (AQPs) are a family of integral membrane proteins which form pores in cell membranes and take part in the transport of water, contributing to the maintenance of water and electrolyte balance and are widely distributed in various tissues and organs. The high expression of AQPs has been described in the digestive system, where large-scale absorption and secretion of fluids occurs. AQPs are also present in the nervous system, but the majority of studies have involved the central nervous system. This paper is a review of the literature concerning relatively little-known issues, i.e. the distribution and functions of AQPs in nervous structures supplying the digestive organs.

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Section: Humanssupporting

confidence: 85%

Section: Humanssupporting

confidence: 52%

Section: Humansmentioning

confidence: 54%

Section: Humansmentioning

confidence: 99%

Section: Possible Functions Of Aqps In the Nervous Structures Supplyimentioning

confidence: 99%

See 3 more Smart Citations

Aquaporins in the nervous structures supplying the digestive organs – a review

Gonkowski

2021

Annals of Animal Science

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AQP1 Is Up-Regulated by Hypoxia and Leads to Increased Cell Water Permeability, Motility, and Migration in Neuroblastoma

Huo

Lomora

Kym

et al. 2021

Front. Cell Dev. Biol.

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The water channel aquaporin 1 (AQP1) has been implicated in tumor progression and metastasis. It is hypothesized that AQP1 expression can facilitate the transmembrane water transport leading to changes in cell structure that promote migration. Its impact in neuroblastoma has not been addressed so far. The objectives of this study have been to determine whether AQP1 expression in neuroblastoma is dependent on hypoxia, to demonstrate whether AQP1 is functionally relevant for migration, and to further define AQP1-dependent properties of the migrating cells. This was determined by investigating the reaction of neuroblastoma cell lines, particularly SH-SY5Y, Kelly, SH-EP Tet-21/N and SK-N-BE(2)-M17 to hypoxia, quantitating the AQP1-related water permeability by stopped-flow spectroscopy, and studying the migration-related properties of the cells in a modified transwell assay. We find that AQP1 expression in neuroblastoma cells is up-regulated by hypoxic conditions, and that increased AQP1 expression enabled the cells to form a phenotype which is associated with migratory properties and increased cell agility. This suggests that the hypoxic tumor microenvironment is the trigger for some tumor cells to transition to a migratory phenotype. We demonstrate that migrating tumor cell express elevated AQP1 levels and a hypoxic biochemical phenotype. Our experiments strongly suggest that elevated AQP1 might be a key driver in transitioning stable tumor cells to migrating tumor cells in a hypoxic microenvironment.

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AQP1 in the Gastrointestinal Tract of Mice: Expression Pattern and Impact of AQP1 Knockout on Colonic Function

Volkart

Kym

Braissant

et al. 2023

IJMS

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Aquaporin 1 (AQP1) is one of thirteen known mammalian aquaporins. Its main function is the transport of water across cell membranes. Lately, a role of AQP has been attributed to other physiological and pathological functions including cell migration and peripheral pain perception. AQP1 has been found in several parts of the enteric nervous system, e.g., in the rat ileum and in the ovine duodenum. Its function in the intestine appears to be multifaceted and is still not completely understood. The aim of the study was to analyze the distribution and localization of AQP1 in the entire intestinal tract of mice. AQP1 expression was correlated with the hypoxic expression profile of the various intestinal segments, intestinal wall thickness and edema, as well as other aspects of colon function including the ability of mice to concentrate stools and their microbiome composition. AQP1 was found in a specific pattern in the serosa, the mucosa, and the enteric nervous system throughout the gastrointestinal tract. The highest amount of AQP1 in the gastrointestinal tract was found in the small intestine. AQP1 expression correlated with the expression profiles of hypoxia-dependent proteins such as HIF-1α and PGK1. Loss of AQP1 through knockout of AQP1 in these mice led to a reduced amount of bacteroidetes and firmicutes but an increased amount of the rest of the phyla, especially deferribacteres, proteobacteria, and verrucomicrobia. Although AQP-KO mice retained gastrointestinal function, distinct changes regarding the anatomy of the intestinal wall including intestinal wall thickness and edema were observed. Loss of AQP1 might interfere with the ability of the mice to concentrate their stool and it is associated with a significantly different composition of the of the bacterial stool microbiome.

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Water permeability is a measure of severity in acute appendicitis

Cited by 3 publications

References 28 publications

Aquaporins in the nervous structures supplying the digestive organs – a review

Aquaporins in the nervous structures supplying the digestive organs – a review

AQP1 Is Up-Regulated by Hypoxia and Leads to Increased Cell Water Permeability, Motility, and Migration in Neuroblastoma

AQP1 in the Gastrointestinal Tract of Mice: Expression Pattern and Impact of AQP1 Knockout on Colonic Function

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