Water near bioinert self-assembled monolayers

Chang, Ryongsok; Asatyas, Syifa; Lkhamsuren, Ganchimeg; Hirohara, Makoto; Mondarte, Evan Angelo Quimada; Suthiwanich, Kasinan; Sekine, Tomoko; Hayashi, T.

doi:10.1038/s41428-018-0075-1

Cited by 22 publications

(24 citation statements)

References 69 publications

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“…The results of the adsorption tests with proteins of single composition ( Figure 5) showed that EG3-OH exhibited strong resistance to fibrinogen and BSA, whereas the other SAMs adsorbed these proteins. These results are consistent with previous findings reported from our group and others [33][34][35][36][37][38] showing that SAMs of oligo(ethyleneglycol)-terminated alkanethiols exhibit strong proteinresistance to various proteins at relatively low concentrations of protein solution (typically < 2 mg/mL). In contrast with the adsorption of single protein at low concentration, the results of adsorption tests with the serum diluted to 20% with PBS revealed that all the SAMs used in this work adsorbed serum proteins.…”

Section: Resultssupporting

confidence: 93%

Proteomic Analysis of Biomaterial Surfaces after Contacting with Body Fluids by MALDI-ToF Mass Spectroscopy

et al. 2019

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We developed a method to identify proteins adsorbed on solid surfaces from a solution containing a complex mixture of proteins by using Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass (MALDI-ToF mass) spectroscopy. In the method, we performed all procedures of peptide mass fingerprint method including denaturation, reduction, alkylation, digestion, and spotting of matrix on substrates. The method enabled us to avoid artifacts of pipetting that could induce changes in the composition. We also developed an algorithm to identify the adsorbed proteins. In this work, we demonstrate the identification of proteins adsorbed on self-assembled monolayers (SAMs). Our results show that the composition of proteins on the SAMs critically depends on the terminal groups of the molecules constituting the SAMs, indicating that the competitive adsorption of protein molecules is largely affected by protein-surface interaction. The method introduced here can provide vital information to clarify the mechanism underlying the responses of cells and tissues to biomaterials.

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Section: Resultssupporting

confidence: 93%

Proteomic Analysis of Biomaterial Surfaces after Contacting with Body Fluids by MALDI-ToF Mass Spectroscopy

et al. 2019

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“…Figure 2B compares the amount of the adsorbed fibrinogen after rinsing, displaying the clear contrast in the degree of protein resistance, in good agreement with the report by Chen et al (2009). In particular, the protein-resistance of EK and DK SAMs are comparable to that of the SAMs comprised of oligo (ethylene glycol)-or sulfobetaine-terminated alkanethiols, which are often widely used for the suppression of non-specific adsorption in biosensing (Hayashi et al, 2012;Tanaka et al, 2013;Sekine et al, 2015;Chang et al, 2018;Kwaria et al, 2020;Hayashi, 2021;Tanaka et al, 2021). The Δd/Δf values also provide insight into the mechanical properties of the protein layer formed on the peptide-SAMs (Table 3).…”

Section: Fibrinogen Adsorption Testsupporting

confidence: 82%

Protein- and Cell-Resistance of Zwitterionic Peptide-Based Self-Assembled Monolayers: Anti-Biofouling Tests and Surface Force Analysis

et al. 2021

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Peptide-based self-assembled monolayers (peptide-SAMs) with specific zwitterionic amino acid sequences express an anti-biofouling property. In this work, we performed protein adsorption and cell adhesion tests using peptide-SAMs with repeating units of various zwitterionic pairs of amino acids (EK, DK, ER, and DR). The SAMs with the repeating units of EK and DK (EK and DK SAMs) manifested excellent bioinertness, whereas the SAMs with the repeating units of ER and DR (ER and DR SAMs) adhered proteins and cells. We also performed surface force measurements using atomic force microscopy to elucidate the mechanism underlying the difference in the anti-biofouling property. Our measurements revealed that water-induced repulsion with a range of about 8 nm acts between EK SAMs (immobilized on both probe and substrate) and DK SAMs, whereas such repulsion was not observed for ER and DR SAMs. The strength of the repulsion exhibited a clear correlation with the protein- and cell-resistance of the SAMs, indicating that the interfacial water in the vicinity of EK and DK SAMs is considered as a physical barrier to deter protein and cells from their adsorption or adhesion. The range of the repulsion observed for EK and DK SAMs is longer than 8 nm, indicating that the hydrogen bonding state of the interfacial water with a thickness of 4 nm is modified by EK and DK SAMs, resulting in the expression of the anti-biofouling property.

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“…It is hypothesized that hydration of the OEG-SAM 30 generated a repulsive force 32 which prevented the DPPC bilayer from approaching the surface. These forces have been implicated in the non-fouling properties of OEG-SAM coated surfaces previously 28 .…”

Section: Sammentioning

confidence: 95%

“…Terminal group charge functionalised oligo (ethylene glycol) alkane thiol self-assembled-monolayers (OEG-SAMs) as were used as supports for membrane deposition. OEG-SAMs have been of interest due to their non-fouling properties 27,28 . Although still not fully understood, many studies suggest that a tightly bound hydration layer between SAM and the bulk interface prevents surface fouling by hindering the binding of solution solubilized/suspended species to the monolayer surface 29,30,31,32 .…”

Section: Alkane Thiol Coating Of the Gold Surfacesmentioning

confidence: 99%

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

Clifton

Paracini²,

Hughes³

et al. 2019

Preprint

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<p>We present a reliable method for the fabrication of fluid phase unsaturated bilayers which are readily self-assembled on charged self-assembled monolayer (SAM) surfaces producing high coverage floating supported bilayers where the membrane to surface distance could be controlled with nanometer precision. Vesicle fusion was used to deposit the bilayers onto anionic SAM coated surfaces. Upon assembly the bilayer to SAM solution interlayer thickness was 7-10 Å with evidence suggesting that this layer was present due to SAM hydration repulsion of the bilayer from the surface. This distance could be increased using low concentrations of salts which caused the interlayer thickness to enlarge to ~33 Å. Reducing the salt concentration resulted in a return to a shorter bilayer to surface distance. These accessible and controllable membrane models are well suited to a range of potential applications in biophysical studies, bio-sensors and Nano-technology.</p><br>

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Water near bioinert self-assembled monolayers

Cited by 22 publications

References 69 publications

Proteomic Analysis of Biomaterial Surfaces after Contacting with Body Fluids by MALDI-ToF Mass Spectroscopy

Proteomic Analysis of Biomaterial Surfaces after Contacting with Body Fluids by MALDI-ToF Mass Spectroscopy

Protein- and Cell-Resistance of Zwitterionic Peptide-Based Self-Assembled Monolayers: Anti-Biofouling Tests and Surface Force Analysis

Self-Assembled Fluid Phase Floating Membranes with Tuneable Water Interlayers

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