Water Modulation of Lens Epithelial Cells During Cataract Surgery

Abstract

CORRESPONDENCE 2. David R, Yagev R, Schneck M, et al. The fate of eyes with culture dish (Figure 1). The cells were then exposed anterior chamber intra-ocular lenses. Eur J Ophthalmol to treatment with distilled deionized water. Within 1993; 3:42-46 60 seconds of exposure, the LECs were markedly swollen 3. Haider S. Spontaneous extrusion of an intraocular lens with grossly morphologically intact cell membranes (Figure implant. J Cataract Refract Surg 1992; 18:529-530 2). At 90 seconds, there was clear disruption of the cell 4. McKnight GT, Richards SC, Apple DJ, et al. Transcorneal

Sealed-capsule Irrigation with Distilled Deionized Water to Prevent Posterior Capsule Opacification – Prospective, Randomized Clinical Trial

“…12 However, shorter exposure time leads to the development of vacuolar degeneration and damage within cellular organelles and it may not guarantee the inhibition of LEC proliferation in long-term follow-up, especially as there comes to the lysis of all LECs, probably between 3 and 6 0 exposure to DW. 8,[11][12][13] The AcrySof SN60WF IOL applied in the study has optimal biocompatibility and physicochemical properties which allows to prevent LEC proliferation (by creating a mechanical barrier), particularly to the central zone of posterior capsule. 2,[27][28][29] The mean values of Total PCO score in a two-year follow-up calculated according to the EPCO system in the case of IOLs constructed on the basis of AcrySof platform are low and are within the limit from 0.145 to 0.478.…”

“…[7][8][9][10][11][12] Unfortunately, toxicity of the applied preparations concerns all eye structures, particularly endothelial cells, which is a marked limitation in their practical use. 11,[13][14][15] Perfect Capsule TM device (Milvella Pty Ltd) used for sealed-capsule irrigation (SCI) creates higher safety for eye structures. 16,17 This system allows to isolate the intracapsular space from the anterior chamber by sealing the lens capsule with a silicone disc attached to capsulorhexis edges and simultaneously to irrigate the space with different chemical agents.…”

Selective Death of Lens Epithelial Cells Using Demineralized Water and Triton X-100 With PerfectCapsule Sealed Capsule Irrigation

“…27,28 The rationale for these agents is that DWI induces cell lysis by osmotic stress. 28 Triton X-100 (TTX-100, 1% vol/vol; Amresco, Solon, Ohio) is an extremely toxic, nonspecific detergent that lyses cell walls, resulting in irreversible cell destruction. The present experimental study was designed to assess the ability to deliver TTX-100 (mixed with DWI) directly to the LECs after crystalline lens removal in rabbit eyes using the sealed (with PerfectCapsule) vs nonsealed conventional irrigation of the capsular bag.…”

“…However, with the development of PerfectCapsule based on the principle of SCI, it is now possible to precisely deliver the pharmacologic/ hypo-osmolar agents to the LECs within the capsular bag while minimizing the potential for collateral ocular damage. 27,28 This current pilot study was designed to assess the immediate effect of a toxin (TTX-100) in rabbit eyes. Rabbits are a well-investigated, accelerated model for LEC activity after cataract surgery owing to the aggressive nature of the LEC proliferation that occurs.…”

Spontaneous closure of Nd:YAG posterior capsulotomy in capsular blockage syndrome

“…It is our experience of human lens capsule culture that LECs will Eye rapidly migrate across the human capsule within 1 week. 11 Potentially, LECs migrate onto exposed capsule or along the anterior hyaloid face, closing the capsulotomy opening. We recommend that, if a posterior capsulotomy is required in the early postoperative period, consideration be given to perform a larger initial capsulotomy.…”