Water mite assemblages reveal diverse genera, novel DNA barcodes and transitional periods of intermediate disturbance

Vasquez, Adrian A.; Carmona-Galindo, Víctor D.; Qazazi, Milad S.; Walker, Xavier N.; Ram, Jeffrey L.

doi:10.1007/s10493-020-00476-4

Cited by 7 publications

(16 citation statements)

References 38 publications

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“…Blue Heron Lagoon is home to at least two species of Lebertia water mites which we have reported in previous studies [20] and further confirmed in this work by DNA barcoding using the "universal metazoan" barcode primers (HCO2198 and LCO1490) of Folmer et al [25,29]. We identified the barcodes of L. quinquemaculosa and L. davidcooki (Fig 1A , 1B, and 1C, 1D, respectively; GenBank accession IDs MG773261.1 and MG773262.1, respectively).…”

Section: Lebertia Water Mite Species From Blue Heron Lagoon Detroitmentioning

confidence: 85%

“…Water mites were collected on multiple dates between July 2016 and March 2017 (see S1 Table ) from Blue Heron Lagoon (latitude 42.344661, longitude -82.959324), on Belle Isle, an island park of the State of Michigan located in the Detroit River, by dragging a 200 μm net along the bottom and through emergent vegetation in depths of 0.5-1 m, washed on a 250 μm sieve, and transported to the Wayne State University Belle Isle Aquarium Field Research Laboratory within 30 minutes of collection. Live water mites were immediately picked on sorting trays and preserved by blanching followed by immersion in 70-90% ethanol and storage at 4 o C, as previously described [20]. Mites were sorted according to genus and species (Lebertia was our study model) and isolated from the rest of the sample as done in our prior studies [20].…”

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

“…Live water mites were immediately picked on sorting trays and preserved by blanching followed by immersion in 70-90% ethanol and storage at 4 o C, as previously described [20]. Mites were sorted according to genus and species (Lebertia was our study model) and isolated from the rest of the sample as done in our prior studies [20]. Each mite was washed with ethanol to avoid cross-contamination from other organisms.…”

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

“…Whole mite DNA was extracted by puncturing individual water mites with sterile minutien pins to allow water mite lysate, including gut contents, to ooze out. DNA was obtained from the punctured mites by lysing and extracting with Proteinase K solution overnight (8-12 hours at 57 o C), and purifying DNA using a Qiagen DNA spin-column protocol (https://www.qiagen.com/us/resources/resourcedetail?id=6b09dfb8-6319-464d-996c-79e8c7045a50&lang=en), followed by storage of the extracted DNA at -20 o C [20]. A voucher of the exoskeleton was retained for morphological analysis and is being held at our laboratory in the Physiology Department, Wayne State University School of Medicine.…”

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

“…They have a complex life cycle in which the larvae of most water mite species parasitize adult aquatic more diverse ingested species beyond that shown in laboratory-based feeding experiments and thereby reveal the natural constituents of water mite diets. Two species of Lebertia were chosen as the focus of this molecular water mite diet studies because of their year-round seasonal availability, previous reports on their predation of aquatic Diptera larvae of ecological importance, abundance and diversity in a local freshwater lagoon [7,[19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Molecular diet studies of water mites reveal prey biodiversity

Vasquez

Mohiddin

et al. 2021

PLoS ONE

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Water mites are diverse aquatic invertebrates that provide potentially important ecosystem and economic services as bioindicators and mosquito biocontrol; however, little is known about water mite digestive physiology, including their diet in nature. Water mites, much like their spider relatives, liquefy their prey upon consumption. This results in the absence of morphologically identifiable prey in water mite mid-gut. Previous studies have reported associations in the field of water mites with presumed prey and laboratory observations of water mites feeding on specific organisms offered for ingestion; however, the present work aims to determine what water mites have ingested in nature based on molecular studies of gut contents from freshly collected organisms from the field. To elucidate water mite prey, we used next-generation sequencing to detect diverse cytochrome oxidase I DNA barcode sequences of putative prey in the guts of 54 specimens comprising two species of Lebertia and a few specimens of Arrenurus (2) and Limnesia (1). To our knowledge this is the first molecular study of the diets of water mites as they feed in nature. While the presence of chironomid DNA confirmed previous observations of midge larvae as part of the diets of Lebertia, we also found the DNA of diverse organisms in all four species of water mites, including the DNA of mosquitoes in 6 specimens of Lebertia and a large number of previously unknown prey, especially from oligochaete worms. These studies thereby reveal a greater diversity of prey and a potentially broader significance than previously appreciated for water mites in aquatic food webs. Molecular studies like this can detect water mite predators of mosquito larvae and add knowledge of water mite predatory contributions to freshwater food webs.

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Section: Lebertia Water Mite Species From Blue Heron Lagoon Detroitmentioning

confidence: 85%

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

Section: Water Mite Collection and Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular diet studies of water mites reveal prey biodiversity

Vasquez

Mohiddin

et al. 2021

PLoS ONE

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Detroit River Phytoplankton Analysis From Water Treatment Plant Data

Vasquez¹,

Ahmed²,

Carmona-Galindo³

et al. 2021

Journal AWWA

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Key Takeaways A new study of phytoplankton in the Detroit River follows significant changes to ecological and environmental conditions in the boundary waters and updates historical data research. Measures to remediate river health in recent years can be evaluated through analysis of the abundance and diversity of phytoplankton communities at a higher taxonomic resolution. By working with community partners to measure phytoplankton communities in the long term, water treatment plants can use the data gained to design an early‐warning system for river changes.

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Diversity and Parasitic Load of Freshwater Fish of the Shipstern Peninsula, Sarteneja, Corozal District, Belize, Central America from Recent Collections and Historical Archives

Ram¹,

Vasquez²,

Trejo-Martinez³

2022

Preprint

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: Belize is located within the Mesoamerican biodiversity hotspot and is an important link between critical biodiverse habitats in Central America. Despite considerable research on biodiversity in marine environments of Belize, research in freshwater environments is limited, with the most recent checklists having been published in obscure, mostly unavailable references more than 20 years ago.Belize is currently experiencing serious degradation of some of its major freshwater resources such as the New River in the northern part of the country. These unique habitats are increasingly threatened by agro-urban development, invasive species and erratic climatic events. The economically important barrier coral reef is also experiencing impacts from the degraded freshwater watersheds that empty into the sea near the reefs. This work addresses the paucity of documentation of the freshwater fishes of Belize, particularly for the Shipstern Peninsula area. The Shipstern Peninsula is a unique region in northern Belize, adjacent to the southern border of Mexico and the northeastern end of the Corozal Bay Wildlife Sanctuary. This paper reports on fish collected in 2015, with comparisons to historical data in surveys published in 1990, 1993, and 1997. We report on 12 genera/species collected in 2015 and provide molecular barcode data on several, which was not available in previous publications. Floridichthys polyommus, a new observation for the Shipstern Lagoon, and Dajaus monticola, a revised name and new observation for the Inland Blue Hole, were confirmed by barcodes. This update is vital to the continued management of freshwater environments in Belize, and especially for the Shipstern Peninsula and its important habitats held in trust for perpetuity for the Belizean people.

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Water mite assemblages reveal diverse genera, novel DNA barcodes and transitional periods of intermediate disturbance

Cited by 7 publications

References 38 publications

Molecular diet studies of water mites reveal prey biodiversity

Molecular diet studies of water mites reveal prey biodiversity

Detroit River Phytoplankton Analysis From Water Treatment Plant Data

Diversity and Parasitic Load of Freshwater Fish of the Shipstern Peninsula, Sarteneja, Corozal District, Belize, Central America from Recent Collections and Historical Archives

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