Water immiscible ionic liquids as solvents for whole cell biocatalysis

Pfruender, Holger; Jones, Ross; Weuster‐Botz, Dirk

doi:10.1016/j.jbiotec.2005.12.004

Cited by 161 publications

(111 citation statements)

References 12 publications

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“…The toxicity of certain ILs can also inhibit enzymatic activity of microorganisms due to accumulation in cell membranes (Matsumoto et al 2004;Pham et al 2010;Romero et al 2008). Some ILs also have been shown to inhibit cell growth and cell viability for various microorganisms (Ganske and Bornscheuer 2006;Jing et al 2014;Pernak et al 2003;Pfruender et al 2006;Sendovski et al 2010). Many challenges associated with IL toxicity must be overcome before ILs can be utilized at the commercial scale (Egorova and Ananikov 2014).…”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…90,91 Potent enzymes [e.g., specialized alcohol dehydrogenases (ADHs)] are anchored to the IM while the internal lumen of the BG becomes the reaction space. Re-suspension of the BGs in an aqueous solution with a suitable reduction equivalent allows for proper function of the desired enzyme.…”

mentioning

confidence: 99%

The bacterial ghost platform system

Langemann¹,

et al. 2010

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The Bacterial Ghost (BG) platform technology is an innovative system for vaccine, drug or active substance delivery and for technical applications in white biotechnology. BGs are cell envelopes derived from Gram-negative bacteria. BGs are devoid of all cytoplasmic content but have a preserved cellular morphology including all cell surface structures. Using BGs as delivery vehicles for subunit or DNA-vaccines the particle structure and surface properties of BGs are targeting the carrier itself to primary antigenpresenting cells. Furthermore, BGs exhibit intrinsic adjuvant properties and trigger an enhanced humoral and cellular immune response to the target antigen. Multiple antigens of the native BG envelope and recombinant protein or DNA antigens can be combined in a single type of BG. Antigens can be presented on the inner or outer membrane of the BG as well as in the periplasm that is sealed during BG formation. Drugs or supplements can also be loaded to the internal lumen or periplasmic space of the carrier. BGs are produced by batch fermentation with subsequent product recovery and purification via tangential flow filtration. For safety reasons all residual bacterial DNA is inactivated during the BG production process by the use of staphylococcal nuclease A and/or the treatment with β-propiolactone. After purification BGs can be stored long-term at ambient room temperature as lyophilized product. The production cycle from the inoculation of the pre-culture to the purified BG concentrate ready for lyophilization does not take longer than a day and thus meets modern criteria of rapid vaccine production rather than keeping large stocks of vaccines. The broad spectrum of possible applications in combination with the comparably low production costs make the BG platform technology a safe and sophisticated product for the targeted delivery of vaccines and active agents as well as carrier of immobilized enzymes for applications in white biotechnology.

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“…When substrate concentration makes the reduction ability of enzyme reaching to saturation, the accumulation of product maintains a certain level although substrate concentration increases. At higher substrate concentration, the conversion and stereo selectivity would be inhibited because of the toxicity effect of aromatic compounds and reactive carbonyl groups for cell (Pfruender et al, 2006). Thus, when considering the conversion rate of the acetophenones, stereo selectivity and accumulation of phenethanol, it could be stated that the optimal parameters of asymmetric reduction reaction of acetophenones was 35 mmol/L under 60 g/L cell concentration.…”

Section: Evaluation Analysis Of Asymmetric Reductionmentioning

confidence: 99%

Rhodotorula mucilaginosa as a new biocatalyst for asymmetric reduction of acetophenone

Bi-Hong¹,

Li²,

Xing³

et al. 2012

Afr. J. Microbiol. Res.

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The biocatalysts for asymmetric reduction of aromatic ketones were successfully screened from soil samples polluted by substituted acetophenones. 12 strains could asymmetrically reduce acetophenone into phenethanol, while only strain YS62 possessed the best performance of reducing acetophenone into (S)-1-phenethanol. It was identified as Rhodotorula mucilaginosa based on phenotypic and genetics characteristics and it was used for further asymmetric reduction experiments of acetophenone as a new biocatalyst. R. mucilaginosa YS62 whole-cells could catalyze the asymmetric reduction of acetophenone (35 mM) into (S)-1-phenethanol (31.4 mM) with a conversion rate of 89.7% and enantiomeric excess (e.e.) of 99.9% under 60 g/L YS62 cell, pH 6.5 , 34°C for 30 h and 2% glucose as a co-substrate. These results have shown that R. mucilaginosa YS62 is a promising biocatalyst for the production of optically active phenylethanol derivatives.

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Water immiscible ionic liquids as solvents for whole cell biocatalysis

Cited by 161 publications

References 12 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

The bacterial ghost platform system

Rhodotorula mucilaginosa as a new biocatalyst for asymmetric reduction of acetophenone

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