Water Hyacinth as Carbon Source for the Production of Cellulase by Trichoderma reesei

Abstract

Water hyacinth (Eichhornia crassipes), an aquatic weed common to the subtropic/tropical regions, was utilized as an inexpensive lignocellulosic substrate for production of cellulase by Trichoderma reesei. The effects of process parameters like substrate pretreatment, substrate concentration, initial medium pH, mode of inoculation, and incubation temperature on cellulase production were investigated. Under optimal conditions, a maximal cellulase activity of 0.22 +/- 0.04 IU/ml (approximately 73.3 IU/g cellulose) was recorded at the end of 15-day incubation period. Specific activity of the enzyme was 6.25 IU/mg protein. Hydrolysis of 1% substrate (water hyacinth) using crude enzyme dosage of 1.2 IU/g water hyacinth showed 28.7% saccharification in 1 h. The observations in present study indicate that saccharification of cellulose from water hyacinth was significantly higher by laboratory-produced cellulase than the commercial blend.

Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in cellulolytic activity and saccharification of barley straw

“…Alkali treatment was done using 2 g (2% w/v) of previously grounded barley straw according to the method described by Deshpande [25]. Briefly, the grounded barley straw was subjected to alkali treatment by soaking in NaOH solution at 2% (w/v) for 48 h at ambient temperature.…”

A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design

“…In the present strain, optimization of parameters for simultaneous production of β -glucosidase and endoglucanase from SSF of water hyacinth showed that maximum β -glucosidase production of 137.32 U/mL was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C, whereas highest amount (495 U/mL) of endoglucanase was obtained at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C, which was quite a large amount in comparison to the other reports available [5, 9, 11, 26]. This could be effectively used for bulk production of these enzymes at a nominal cost.…”

“…Water hyacinth (Eichornia crassipes) is one of the fastest growing aquatic weeds that causes huge economic loss by impeding water flow, accelerating water evaporation, increasing mosquito breeding, and destroying rice fields [9]. The approach of utilizing water hyacinth for cellulase production was expected to serve the twin function of otherwise nuisance weeds as well as reduction of enzyme cost [10].…”

Physical and Biophysical Pretreatment of Water Hyacinth Biomass for Cellulase Enzyme Production

“…26 Although the pretreatment methods to produce cellulase have been studied extensively, only a few utilize water hyacinth as a substrate. 24,27 In this study, two methods of pretreatment were employed to water hyacinth. Physical pretreatment was conducted by reducing the particle size by chopping and grinding.…”