Water extracts of cabbage and kale inhibit ex vivo H2O2-induced DNA damage but not rat hepatocarcinogenesis

Abstract

0.05) placental glutathione S-transferase-positive preneoplastic lesions (PNL). The number of apoptotic bodies did not differ (P > 0.05) among the experimental groups. Ex vivo hydrogen peroxide treatment of rat livers resulted in lower (P < 0.05) DNA strand breakage in cabbage- (107.6 ± 7.8 µm) and kale- (110.8 ± 10.0 µm) treated animals compared with control (120.9 ± 12.7 µm), as evaluated by the single cell gel (comet) assay. Treatment with cabbage (2 ± 0.3 µg/g) or kale (4 ± 0.2 µg/g) resulted in increased (P < 0.05) hepatic lutein concentration compared with control (0.5 ± 0.07 µg/g). Despite the absence of inhibitory effects of cabbage and kale aqueous extracts on PNL, these Brassica vegetables presented protection against DNA damage, an effect possibly related to increased hepatic lutein concentrations. However, it must be pointed out that the cause-effect relationship between lutein levels and protection is hypothetical and remains to be demonstrated.]]>

The chemopreventive effect of Ginkgo biloba and Silybum marianum extracts on hepatocarcinogenesis in rats

“…The current investigation demonstrated a highly significant increase in the comet assay parameters (TL,% DNA in tail and TM) in NDEA treated rats compared to the healthy control rats. This is in consistent with the previous results of Horst et al [ 58 ] who reported NDEA-induced DNA damage indicated by the markedly increased length of the comets (head + tail) in the liver cells of rats administrated NDEA compared to the negative control rats. The increment of such parameters may be due to that the enzymes of CYP2E1 subfamily play a role in the biotransformation of a range of compounds, including NDEA, producing the promutagenic DNA lesions which play an important role in DNA damage and induction of hepatocarcinogenesis [ 35 ].…”

A multibiomarker approach in rats to assess the impact of pollution on Sinos River, Southern Brazil

“…Sinos River water samples were administered orally (as drinking water; Horst et al, 2010) for a 48 hours period to rats in the treatment groups. The control group received dechlorinated tap water.…”

Improving the Health-Benefits of Kales (Brassica oleracea L. var. acephala DC) through the Application of Controlled Abiotic Stresses: A Review

“…For instance, Sikora and Bodziarczyk [16] reported lower lipid oxidation products (LOP) and malondialdehyde (MDA) in the blood serum of rats fed with a modified diet with raw and lyophilized kale. Likewise, Horst et al [17] found that kale extract supplementation in Wistar rats (4 ± 0.2 µg/g) showed protection against H 2 O 2 -induced DNA damage. Moreover, Chung et al [18] described that treatment with kale juice powder for 8 weeks improved serum lipid profiles by increasing the HDL level and decreasing the triglycerides level in Sprague Dawley rats previously fed with a high cholesterol diet.…”