Water-extracted polysaccharides from Azadirachta indica leaves: Structural features, chemical modification and anti-bovine herpesvirus type 1 (BoHV-1) activity

Antiherpetic activity of an Agaricus brasiliensis polysaccharide, its sulfated derivative and fractions

“…Of the PLS derivatives presently used, only the sulfated form demonstrated stronger inhibition for both HSV-1 and BoHV-1 replication, when the cells were treated simultaneously to infection. Similar findings were described, for carrageenans [31], fucoidans [32], PLS from Azadirachta indica [33], sulfated ␤-glucans [34] and sulfated derivatives. Overall, we suggest that antiherpetic activity of sulfated polysaccharides could be through different mechanisms, including virucidal and adsorption inhibition effects.…”

“…Co., USA), respectively. The cells were examined in a Zeiss fluorescence microscope (Imager A1 with Axio Cam MRc5) and 100 cells/coverslip were counted and the percentage of fluorescent cells calculated in comparison to control infected and nontreated cells [33].…”

Azadirachta indica

“…azadirachtin, did not reveal any inhibition on dengue virus type 2 replication in both in vitro and in vivo systems. Water-extracted polysaccharides from Azadira chta indica leaves showed activity against bovine herpesvirus type 1 (Saha et al 2010 ). In their inhibitory concentration at 50 %, values ranging from 31.12 to 105.25 μg/mL were lower than the cytotoxicity values (>1,600-1,440 μg/mL).…”

“…)-linked β-D -galactosyl and terminal rhamnosyl and α-L -arabinosyl residues was isolated from Azadirachta indica leaves (Saha et al 2010 ). Nimbolide [systematic name, (4α,5α,6α,7α,15β,17α)-7,15:21,23-diepoxy-6-hydroxy-4,8-dimethyl-1-oxo-18,24-dinor-11,12-secochola-2,13,20,22-tetraene-4,11-dicarboxylic acid gamma-lactone methyl ester] was isolated from neem leaves, and its isomer, isonimbolide [systematic name, (4α,5α,6α,7α,15α)-7,15:21,23-diepoxy-6-hydroxy-4,8-dimethyl-1-oxo-18,24-dinor-11,12-secochola-2,16,20,22-tetraene-4,11-dicarboxylic acid gamma-lactone methyl ester], was prepared from a novel rearrangement reaction of nimbolide using boron trifl uoride etherate and tetrabutylammonium bromide (Solomon et al 2005 ).…”

Purification, preliminary characterization and bioactivities of polysaccharides from Ostrea rivularis Gould

“…All these were characteristic absorptions of polysaccharides. GC analysis showed that ORPp was composed of galactose [26] and glucose (Glu) in a molar percent of 23.7% and 76.3%, respectively (Fig. 2C).…”