Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines

Li, Wing-Yan; Chan, Shun‐Wan; Guo, De-Jian; Chung, Mei-Kuen; Leung, Tin-Yan; Yu, Peter H.

doi:10.1016/j.jep.2009.04.030

Cited by 71 publications

(44 citation statements)

References 15 publications

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“…Emodin is also observed naturally in a number of widely used Chinese medicinal herbs, including Rheum officinale (8) and Polygonam cuspidatummedicine (9). It has been widely studied for its antibacterial, diuretic, immuno suppressive, anti-inflammatory and vaso relaxant effects.…”

Section: Introductionmentioning

confidence: 99%

Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes

Zhang

Hou

et al. 2015

Oncology Letters

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Abstract. The aim of this study was to investigate the effects of emodin on the proliferation of human breast cancer cells . Cell viability following emodin treatment was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of emodin on apoptosis were determined by flow cytometry using Annexin V-fluorescein isothiocyanate and propidium iodide staining. Quantitative polymerase chain reaction and western blot analysis were used to determine changes in the expression of apoptotic genes and protein, respectively. The effect of emodin on the invasiveness of breast cancer cells was evaluated by Matrigel invasion assay. Treatment of breast cancer cells Bcap-37 and ZR-75-30 with emodin was observed to inhibit the growth and induced apoptosis in a time-and dose-dependent manner. Emodin reduced the level of Bcl-2 and increased levels of cleaved caspase-3, PARP, p53 and Bax. These findings indicate that emodin induces growth inhibition and apoptosis in human breast cancer cells. Emodin may be a potential therapeutic agent for the treatment of breast cancer. IntroductionBreast cancer is a common malignant tumor and accounts for the majority of cancer mortality in females (1). Breast cancer has an estimated incidence of 1,676,633 and had an estimated mortality rate of 521,817 in 2012 worldwide (2). In China, there has been an increasing incidence of breast cancer in recent decades (3). In 2012, it was estimated that ~48,000 females would succumb to breast cancer. To date, chemotherapy has been the mainstay for the treatment of breast cancer. However, the side effects and drug resistance associated with chemotherapy have limited its effectiveness for the treatment of breast cancer (4,5). A number of natural extractions have demonstrated a wide range of biological activity and low toxicity in animal models, and have been considered as an alternative method of breast cancer treatment (6,7).Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is a biologically active anthraquinone identified in the roots and bark of a number of plants. Emodin is also observed naturally in a number of widely used Chinese medicinal herbs, including Rheum officinale (8) and Polygonam cuspidatummedicine (9). It has been widely studied for its antibacterial, diuretic, immuno suppressive, anti-inflammatory and vaso relaxant effects. It has been reported that emodin induces apoptosis of several types of human cancer cells (10-13) by modulating various signaling pathways. However, the biological mechanisms by which emodin induces cytotoxicity remain largely unknown. Furthermore, the biological effects of emodin on breast cancer cells remain to be determined.In this study, the effects of emodin on the proliferation and apoptosis of breast cancer cells were assessed. In addition, the mechanism by which emodin mediates these biological effects was elucidated. We demonstrated that emodin inhibited the proliferation and induced apoptosis of Bcap-37 and ZR-75-30 breast cancer cells. These observations suggest that emodi...

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Section: Introductionmentioning

confidence: 99%

Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes

Zhang

Hou

et al. 2015

Oncology Letters

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“…The methanol extract of Scutellaria orientalis showed potent anti-leukemic activity against HL-60 cell line (Ozmen et al, 2010). The water extract of Rheum officinale exhibited significant antiproliferative activity by inducing apoptosis in MCF-7 and A549 cell lines (Li et al, 2009). A potent antiproliferative activity was also reported for the hexane extract of Casearia sylvestris stem bark against different cancer cell lines (Mesquita et al, 2009) and the butanol extract of Pfuffia paniculata demonstrated high cytotoxic activity against MCF-7 cell line (Nagamine et al, 2009).…”

Section: The Use Of Plants In Cancer Therapymentioning

confidence: 83%

Anticancer and Antimicrobial Potential of Plant-Derived Natural Products

Talib¹

2011

Phytochemicals - Bioactivities and Impact on Health

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“…Relevant antimitochondrial effects were demonstrated in some cases, eventually leading to cell apoptosis 7,8,10,11,33 . The colony formation assay was developed to detect the growth inhibitory effects of drugs on the anchorageindependent growth of colonies 18 . There are many studies about the antitumoral effects of Au(III) and Pt(II) compounds, but any of them were carried out for CFA assay.…”

Section: Discussionmentioning

confidence: 99%

“…The antineoplastic activities of [Au(phen)Cl 2 ]Cl and Pt(phen)Cl 2 were investigated through CFA assay as described in Li et al 18 with modifications in A549 cell line. Colony inhibition was calculated with the following formula: Colony forming inhibition % = 100 × (1-colony number of drug treated group/colony number of control group) 19 .…”

Section: Soft Agar Colony Forming Assaymentioning

confidence: 99%

“…CFA was performed using a double-layer soft agar method. The colony formation assay was developed to detect the growth inhibitory effects of drugs on the anchorage-independent growth of colonies 18 . [Au(phen)Cl 2 ]Cl and Pt(phen) Cl 2 treatments were found to exert a concentrationdependent inhibitory effect on the CFA of A549 cells (Figure 2).…”

Section: Soft Agar Colony Forming Assaymentioning

confidence: 99%

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Studies on the cytotoxic, apoptotic and antitumoral effects of Au(III) and Pt(II) complexes of 1, 10-phenanthroline on V79 379A and A549 cell lines

Bostancıoğlu

Işık

Genç

et al. 2011

Journal of Enzyme Inhibition and Medicinal Chemistry

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Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines

Abstract: This suggests that the water extract of Da Huang exerts potential anticancer activity through growth inhibition and apoptosis on MCF-7 and A549 cells lines.

Cited by 71 publications

References 15 publications

Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes

Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes

Anticancer and Antimicrobial Potential of Plant-Derived Natural Products

Studies on the cytotoxic, apoptotic and antitumoral effects of Au(III) and Pt(II) complexes of 1, 10-phenanthroline on V79 379A and A549 cell lines

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