Water droplet surface tension method – An innovation in quantifying saponin content in quinoa seed

Yousif, Adel M.; Snowball, R.; D’Antuono, M.; Dhammu, Harmohinder; Sharma, Darshan

doi:10.1016/j.foodchem.2020.128483

Cited by 5 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the evaluation of the afrosymetric method for the quantification of saponin in quinoa germplasm from the department of Boyacá, it was possible to observe as shown in the Figure 1, the foam height was variable and depended on the material evaluated. However, the afrosymetric method was capable of extracting some proteins that can influence foam stability, where the foam height changed rapidly in both directions, which could lead to an overestimation of saponin contents (León-Roque et al, 2019;Yousif et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…The quantification of this molecule has been carried out with different methods, such as afrosymetric, hemolytic, spectrophotometric, and chromatographic methods, among others (Koziol, 1991;El-Aziz et al, 2019). The Afrosymetric method presented optimal results despite the fact that the high polarity and structural complexity of saponins make their isolation, identification, and quantification a complex process (Yousif et al, 2021). This method is based on the property of saponins that decreases the surface tension of water and forms a relatively stable foam, the height of which correlates with the saponin content in quinoa seeds (Gargiulo et al, 2019;Wisetkomolmat et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the surfactant property of saponins means they can be quantified in quinoa grains with the Afrosymetric method because the formation of a stable and persistent foam at the liquid/air interface indicates the presence of saponins (Amankeldi et al, 2018;Yousif et al, 2021). The standard afrosymetric method was initially developed by Koziol (1991), in the search for a simple technique to extract and determine the presence of saponins in quinoa grains and was used to evaluate more than 5000 quinoa genotypes from Latinreco (Ecuador), with significant results.…”

Section: Modified Afrosymetric Methods (Mam)mentioning

confidence: 99%

See 2 more Smart Citations

Afrosymetric method for quantifying saponins in Chenopodium Quinoa Willd. from Colombia

2022

View full text Add to dashboard Cite

Quinoa (Chenopodium quinoa Willd.) is a pseudocereal that, in addition to presenting good nutritional characteristics in the grain, has secondary metabolites in the episperm of the seeds and, within them, saponins. Saponins are characterized by being emulsifying, foaming and generating a bitter taste. This metabolite has not been completely studied in quinoa materials from Colombia. For this reason, the objective of this research was to quantify the content of saponins present in quinoa materials from the department of Boyacá using three afrosimetric methods. For this, a completely randomized design (CRD) was implemented with a factorial arrangement of 3 (afrosimetric methods) x 5 (quinoa materials). From the quantification of saponins, it was determined that the Amarilla de maranganí genotype, was the one that presented the highest content in all the evaluated methods; the standard afrosimetric method being the most efficient. Finally, the conglomerate analysis allowed to discriminate the materials in sweet quinoas such as Tunkahuan and Blanca de Jericó with saponin contents lower than 0.06%, and in bitter quinoas (Negra de la Colorada, Dorada and Amarilla de Maranganí) with contents higher than 0.11%. The biochemical characterization of the germplasm will allow a selection of genotypes suitable for consumption and for the industry, given the potential use that saponins currently have.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Modified Afrosymetric Methods (Mam)mentioning

confidence: 99%

See 1 more Smart Citation

Afrosymetric method for quantifying saponins in Chenopodium Quinoa Willd. from Colombia

2022

View full text Add to dashboard Cite

show abstract

“…Distilled water was first considered as a control solution. Indeed, the value of the surface tension of water at 0 kV is well known in the literature (Kovalchuk, Alberini, and Simmons 2020;Yousif et al 2021;Liu and Cao 2021): it is 72 mN.m -1 . The measurement of the ST of a droplet of water was used as a reference measurement in order to compare it to the measurements made with two other model solutions.…”

Section: Model Solutionsmentioning

confidence: 99%