“…15 However, the HPLC method also encounters some shortcomings in the sample pretreatment, such as complex process, [19][20][21][22][23][24] long detection cycle 8,25 and low sensitivity. [26][27][28][29][30][31][32] In addition, assessing the toxicity of cancer drugs to biological tissues is very important 5,[33][34][35][36][37][38] and valuable to ensure the health of humans and other creatures. [6][7][8]39,40 In the commonly used biodetection approach, the UCNPs usually need to be injected into the living body, but it is hard to remove from blood vessels, which can be considered as the intrinsic defect of the uorescence resonance energy transfer based biodetection approach.…”