Abstract:Water-dispersible glycosylated poly(2,5′-thienylene)porphyrin-based nanoparticles have the ability to generate singlet oxygen in high yields and exhibit light-triggered antibacterial activity against Gram negative bacteria, E. coli as well as Gram positive bacteria, B. subtilis.
“…We also compared the aPDT performance of TCPP-TG NPs with that of the reported porphyrin-based materials in the literature for the inactivation of E. coli (Figure 3e and Table S1), 36,59−63 and the data listed in Figure 3e showed that the bacterial killing efficiency of TCPP-TG NPs is at least 3−6 orders of magnitude higher than that of other reported porphyrin-based materials. It is worth noting that the irradiation time and the light density used in our study were less intense than the irradiation time (30−120 min) 59,60,62 and the light density (22−90 mW cm −2 ) 36,59,60,63 used in many previously reported porphyrin-based systems.…”
Bacterial infection has become an urgent health problem in the world. Especially, the evolving resistance of bacteria to antibiotics makes the issue more challenging, and thus new treatments to fight these infections are needed. Antibacterial photodynamic therapy (aPDT) is recognized as a novel and promising method to inactivate a wide range of bacteria with few possibilities to develop drug resistance. However, the photosensitizers (PSs) are not effective against Gram-negative bacteria in many cases. Herein, we use conjugated meso-tetra(4-carboxyphenyl)porphine (TCPP) and triaminoguanidinium chloride (TG) to construct self-assembled cationic TCPP-TG nanoparticles (NPs) for efficient bacterial inactivation under visible light illumination. The TCPP-TG NPs can rapidly adhere to both Gram-negative and Gram-positive bacteria and display promoted singlet oxygen ( 1 O 2 ) generation compared with TCPP under light irradiation. The high local positive charge density of TCPP-TG NPs facilitates the interaction between the NPs and bacteria. Consequently, the TCPP-TG NPs produce an elevated concentration of local 1 O 2 under light irradiation, resulting in an extraordinarily high antibacterial efficiency (99.9999% inactivation of the representative bacteria within 4 min). Furthermore, the TCPP-TG NPs show excellent water dispersity and stability during 4 months of storage. Therefore, the rationally designed TCPP-TG NPs are a promising antibacterial agent for effective aPDT.
“…We also compared the aPDT performance of TCPP-TG NPs with that of the reported porphyrin-based materials in the literature for the inactivation of E. coli (Figure 3e and Table S1), 36,59−63 and the data listed in Figure 3e showed that the bacterial killing efficiency of TCPP-TG NPs is at least 3−6 orders of magnitude higher than that of other reported porphyrin-based materials. It is worth noting that the irradiation time and the light density used in our study were less intense than the irradiation time (30−120 min) 59,60,62 and the light density (22−90 mW cm −2 ) 36,59,60,63 used in many previously reported porphyrin-based systems.…”
Bacterial infection has become an urgent health problem in the world. Especially, the evolving resistance of bacteria to antibiotics makes the issue more challenging, and thus new treatments to fight these infections are needed. Antibacterial photodynamic therapy (aPDT) is recognized as a novel and promising method to inactivate a wide range of bacteria with few possibilities to develop drug resistance. However, the photosensitizers (PSs) are not effective against Gram-negative bacteria in many cases. Herein, we use conjugated meso-tetra(4-carboxyphenyl)porphine (TCPP) and triaminoguanidinium chloride (TG) to construct self-assembled cationic TCPP-TG nanoparticles (NPs) for efficient bacterial inactivation under visible light illumination. The TCPP-TG NPs can rapidly adhere to both Gram-negative and Gram-positive bacteria and display promoted singlet oxygen ( 1 O 2 ) generation compared with TCPP under light irradiation. The high local positive charge density of TCPP-TG NPs facilitates the interaction between the NPs and bacteria. Consequently, the TCPP-TG NPs produce an elevated concentration of local 1 O 2 under light irradiation, resulting in an extraordinarily high antibacterial efficiency (99.9999% inactivation of the representative bacteria within 4 min). Furthermore, the TCPP-TG NPs show excellent water dispersity and stability during 4 months of storage. Therefore, the rationally designed TCPP-TG NPs are a promising antibacterial agent for effective aPDT.
“…A red-emitting COL, namely, 3,3′,3″,3‴-{[(1 E ,1′ E )-2,1,3-benzothiadiazole-4,7-diylbis(ethene-2,1-diyl)]-bis(9 H -fluorene-9,9,2-triyl)}-tetrakis( N , N -dimethylpropan-1-amine), was synthesized according to the literature procedure. , ROS generation efficiency was determined using dichlorofluorescein diacetate (DCFH-DA) assay . Preparation of Escherichia coli (E. coli) and Bacillus subtilis ( B. subtilis) suspensions, determination of minimum inhibitory concentration (MIC), photodynamic inactivation of bacteria, and performing scanning electron microscopy (SEM) imaging, ζ-potential measurements, and cytotoxicity assays of nanoparticles were carried out using similar procedures reported in our previous publications, − and details are also given in the Supporting Infomation.…”
Herein,
hybrid nanoparticles composed of a red-emitting conjugated
oligomer (COL) and gold nanoparticles (Au-NPs) were prepared through
a one-pot synthetic method in which the oligomer acts as a reducing
agent as well as a matrix to wrap the newly formed Au nanoparticles.
These hybrid nanoparticles (COL-Au-NPs) exhibited photodynamic and
photothermal activity against both Gram-positive and Gram-negative
bacterial strains. They were also proven to possess high photostability
and thermal reversibility. Dark cytotoxicity of COL-Au-NPs toward
pathogens and mammalian breast cancer cells (MCF-7) reduced significantly
upon complexation with cucurbit[7]uril while preserving their light-induced
cytotoxic activity when irradiated with a 915 nm laser for photothermal
therapy and white light for photodynamic therapy, respectively. Furthermore,
these nanoparticles have cellular imaging capability because of their
intrinsic fluorescence characteristics and can be used in image-guided
therapy.
“…The glycosylation of PSs is expected to acquire stability as well as additional benefits such as cell-surface interactions and site-specific delivery. Water-soluble glycopolymers potentially increase the PDI efficacies of non-porphyrin PSs by promoting their bacterial adherence [ 32 , 71 , 72 ]. Fig.…”
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