Water-dispersed luminescent quantum dots for miRNA detection

Goryacheva, Olga A.; Novikovа, A. S.; Drozd, Daniil D.; Pidenko, Pavel S.; Ponomaryeva, T.S.; Bakal, Artem A.; Mishra, Pradyumna Kumar; Beloglazova, Natalia V.; Goryacheva, Irina Yu.

doi:10.1016/j.trac.2018.12.022

Cited by 33 publications

(11 citation statements)

References 72 publications

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“…The optical characteristics of GQDs can be used in biosensing; this application uses the PL of GQDs and generally requires photon detection . GQDs, serve the purpose of detecting and indicating the presence of nucleic acid biomarkers in biosensing systems . GQD-based biosensors utilize the affinity between specific functional groups within GQDs and the analyte biomolecule.…”

Section: Biosensing Techniques For the Detection Of Ccfnasmentioning

confidence: 99%

“… 122 GQDs, serve the purpose of detecting and indicating the presence of nucleic acid biomarkers in biosensing systems. 123 GQD-based biosensors utilize the affinity between specific functional groups within GQDs and the analyte biomolecule. When a functional group conjugated onto the GQDs binds to the analyte, the association between the pair can provide different electronic states.…”

Section: Biosensing Techniques For the Detection Of Ccfnasmentioning

confidence: 99%

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Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

et al. 2022

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Graphene quantum dots (GQDs) are carbonaceous nanodots that are natural crystalline semiconductors and range from 1 to 20 nm. The broad range of applications for GQDs is based on their unique physical and chemical properties. Compared to inorganic quantum dots, GQDs possess numerous advantages, including formidable biocompatibility, low intrinsic toxicity, excellent dispensability, hydrophilicity, and surface grating, thus making them promising materials for nanophotonic applications. Owing to their unique photonic compliant properties, such as superb solubility, robust chemical inertness, large specific surface area, superabundant surface conjugation sites, superior photostability, resistance to photobleaching, and nonblinking, GQDs have emerged as a novel class of probes for the detection of biomolecules and study of their molecular interactions. Here, we present a brief overview of GQDs, their advantages over quantum dots (QDs), various synthesis procedures, and different surface conjugation chemistries for detecting cell-free circulating nucleic acids (CNAs). With the prominent rise of liquid biopsy-based approaches for real-time detection of CNAs, GQDs-based strategies might be a step toward early diagnosis, prognosis, treatment monitoring, and outcome prediction of various non-communicable diseases, including cancers.

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Section: Biosensing Techniques For the Detection Of Ccfnasmentioning

confidence: 99%

Section: Biosensing Techniques For the Detection Of Ccfnasmentioning

confidence: 99%

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

et al. 2022

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“…Quantum dots (QDs) are luminescent semiconductor nanocrystals with the possibility to tune the maximum wavelength of their light emission spectra. Stable QDs can be obtained by an easy one-pot and/or one-step synthesis directly in a water medium [ 28 ]. Aqueous suspensions of QDs have a high photoluminescent quantum yield and resistance to photobleaching [ 29 ].…”

Section: Nanomaterials Used As Substratesmentioning

confidence: 99%

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Vilímová¹,

Hervé-Aubert²,

Chourpa³

2022

Molecules

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Recently, microRNAs (miRNA) captured the interest as novel diagnostic and prognostic biomarkers, with their potential for early indication of numerous pathologies. Since miRNA is a short, non-coding RNA sequence, the sensitivity and selectivity of their detection remain a cornerstone of scientific research. As such, methods based on nanomaterials have emerged in hopes of developing fast and facile approaches. At the core of the detection method based on nanotechnology lie nanoprobes and other functionalized nanomaterials. Since miRNA sensing and detection are generally rooted in the capture of target miRNA with the complementary sequence of oligonucleotides, the sequence needs to be attached to the nanomaterial with a specific conjugation strategy. As each nanomaterial has its unique properties, and each conjugation approach presents its drawbacks and advantages, this review offers a condensed overview of the conjugation approaches in nanomaterial-based miRNA sensing. Starting with a brief recapitulation of specific properties and characteristics of nanomaterials that can be used as a substrate, the focus is then centered on covalent and non-covalent bonding chemistry, leading to the functionalization of the nanomaterials, which are the most commonly used in miRNA sensing methods.

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“…Conjugating a recognition motif in DNA probes is effective for the detection of various targets. , However, conventional DNA probes may require additional labels such as organic dyes or quantum dots as fluorescent reporters. − A labeling process may require costly reagents and multiple steps of purification. The external fluorescent sources also have negative influences on detection performance such as sensitivity, selectivity, and accuracy.…”

Section: Overall Perspective On the Detection Of Viruses Based On Dna...mentioning

confidence: 99%

Development of General Methods for Detection of Virus by Engineering Fluorescent Silver Nanoclusters

Chen

Gao

et al. 2021

ACS Sens.

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Viruses have caused significant damage to the world. Effective detection is required to relieve the impact of viral infections. A biomolecule can be used as a template such as deoxyribonucleic acid (DNA), peptide, or protein, for the growth of silver nanoclusters (AgNCs) and for recognizing a virus. Both the AgNCs and the recognition elements are tunable, which is promising for the analysis of new viruses. Considering that a new virus such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) urgently requires a facile sensing strategy, various virus detection strategies based on AgNCs including fluorescence enhancement, color change, quenching, and recovery are summarized. Particular emphasis is placed on the molecular analysis of viruses using DNA stabilized AgNCs (DNA-AgNCs), which detect the virus's genetic material. The more widespread applications of AgNCs for general virus detection are also discussed. Further development of these technologies may address the challenge for facile detection of SARS-CoV-2.

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Water-dispersed luminescent quantum dots for miRNA detection

Cited by 33 publications

References 72 publications

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

Bioanalytical Applications of Graphene Quantum Dots for Circulating Cell-Free Nucleic Acids: A Review

Formation of miRNA Nanoprobes—Conjugation Approaches Leading to the Functionalization

Development of General Methods for Detection of Virus by Engineering Fluorescent Silver Nanoclusters

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