Water Content of Plant Tissues: So Simple That Almost Forgotten?

Ievinsh, Gederts

doi:10.3390/plants12061238

Cited by 28 publications

(24 citation statements)

References 182 publications

(215 reference statements)

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“…This bound water level is a fingerprint of the specific biomolecules present and their water-binding properties, as shown in previous research. [44][45][46][47] Since these factors vary significantly between different species within the Allium genus, measuring their species-specific evaporation profiles using the rapid THz-LFI imaging method could offer a novel approach for identifying other genetic variants.…”

Section: Resultsmentioning

confidence: 99%

Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Kundu,

Tornainen,

Bertling

et al. 2024

Photonic Technologies in Plant and Agricultural Science

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In this article, we report our work on the development of a non-invasive, rapid, robust, and high-fidelity technique that can be used to discriminate between genetic variants. Our study focused on terahertz (THz) spectroscopy and imaging to distinguish between genetic variants of the Allium genus rapidly and accurately. This was done by measuring the cellular water dynamics of the samples by measuring their evaporation profiles using laser feedback interferometry (LFI) with THz quantum cascade lasers (QCL). The evaporation profiles of the samples were then processed to create trajectories in the amplitude-phase domain, which correlated with cell age, cell type, and the amount of water bound to biomolecules. This technique can differentiate between the members of the Allium genus. The presence of outliers was also studied to determine the effectiveness of the technique for different samples and to negate external influence. This was done to discern the extent of influence of cell biomechanics and biochemistry between genetic variants. We found that within a genus, different species would have different degree of interaction between cellular water and cell biochemistry, which could be clearly mapped out using THz-QCL-based LFI. Based on our observations, we propose that this method could be appropriate for observing minute alterations in cellular water dynamics in real-time, and in the future, has the potential to be employed for rapid and effective genetic discrimination in agricultural and genome conservation applications.

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Section: Resultsmentioning

confidence: 99%

Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Kundu,

Tornainen,

Bertling

et al. 2024

Photonic Technologies in Plant and Agricultural Science

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“…The different parts of the plant, like the stem, roots, and leaves, share a similar role in all species. For plants to exhibit mechanical properties, the location of water in tissues and cells is important . Essentially, they all transport water throughout the plant in near identical ways as well as store it in the large central vacuoles in all plant cells.…”

Section: Resultsmentioning

confidence: 99%

“…For plants to exhibit mechanical properties, the location of water in tissues and cells is important. 37 Essentially, they all transport water throughout the plant in near identical ways as well as store it in the large central vacuoles in all plant cells. This study looks at one metabolic pathway as a model system, but the detection and localization of other metabolites can be measured utilizing these methods.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Incorporation of Three Different Optical Trains into the IR-MALDESI Mass Spectrometry Imaging Platform to Characterize Artemisia annua

Ashbacher,

Mills,

Sohn

et al. 2024

J. Am. Soc. Mass Spectrom.

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Artemisinin is the leading medication for the treatment of malaria and is only produced naturally in Artemisia annua. The localization of artemisinin in both the glandular and non-glandular trichomes of the plant makes it an ideal candidate for mass spectrometry imaging (MSI) as a model system for method development. Infrared matrix-assisted laser desorption electrospray ionization MSI (IR-MALDESI-MSI) has the capability to detect hundreds to thousands of analytes simultaneously, providing abundance information in conjunction with species localization throughout a sample. The development of several new optical trains and their application to the IR-MALDESI-MSI platform has improved data quality in previous proof-of-concept experiments but has not yet been applied to analysis of native biological samples, especially the MSI analysis of plants. This study aimed to develop a workflow and optimize MSI parameters, specifically the laser optical train, for the analysis of Artemisia annua with the NextGen IR-MALDESI platform coupled to an Orbitrap Exploris 240 mass spectrometer. Two laser optics were compared to the conventional set up, of which include a Schwarzschild-like reflective objective and a diffractive optical element (DOE). These optics, respectively, enhance the spatial resolution of imaging experiments or create a square spot shape for top-hat imaging. Ultimately, we incorporated and characterized three different optical trains into our analysis of Artemisia annua to study metabolites in the artemisinin pathway. These improvements in our workflow, resulted in high spatial resolution and improved ion abundance from previous work, which will allow us to address many different questions in plant biology beyond this model system.

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“…Moisture content refers to the amount of water present in a sample ( 52 ) Water has been known to perform numerous functions such as temperature regulation, transport function, medium for substrate dissolution and lots more ( 53 ). The moisture content of fresh and dry Pleurotus ostreatus was 91.01 and 6.46%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Assessing the nutritional quality of Pleurotus ostreatus (oyster mushroom)

Effiong,

Umeokwochi,

Afolabi

et al. 2024

Front. Nutr.

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There is a huge gap between food production and the exploding population demands in various parts of the world, especially developing countries. This increases the chances of malnutrition, leading to increased disease incidence and the need for functional foods to reduce mortality. Pleurotus ostreatus are edible mushrooms that are cheaply sourced and rich in nutrient with the potential to be harnessed toward addressing the present and future food crisis while serving as functional foods for disease prevention and treatment. This study evaluated the nutritional, proximate, vitamins and amino acids contents of Pleurotus ostreatus. The proximate composition of Pleurotus ostreatus in this study revealed that it contains 43.42% carbohydrate, 23.63% crude fiber, 17.06% crude protein, 8.22% ash, 1.21% lipid and a moisture content of 91.01 and 6.46% for fresh and dry samples of Pleurotus ostreatus, respectively. The monosaccharide and disaccharide profile of Pleurotus ostreatus revealed the presence of glucose (55.08 g/100 g), xylose (7.19 g/100 g), fructose (19.70 g/100 g), galactose (17.47 g/100 g), trehalose (7.37 g/100 g), chitobiose (11.79 g/100 g), maltose (29.21 g/100 g), sucrose (51.60 g/100 g) and lower amounts of cellobiose (0.01 g/100 g), erythrose (0.48 g/100 g) and other unidentified sugars. Potassium, Iron and Magnesium were the highest minerals present with 12.25 mg, 9.66 mg and 7.00 mg amounts, respectively. The vitamin profile revealed the presence of vitamin A (2.93 IU/100 g), C (16.46 mg/100 g), E (21.50 mg/100 g) and B vitamins with vitamin B2 having the highest concentration of 92.97 mg/kg. The amino acid scores showed that Pleurotus ostreatus had more non-essential amino acids (564.17 mg/100 g) than essential amino acids (67.83 mg/100 g) with a ratio of 0.11. Lysine (23.18 mg/100 g) was the highest essential amino acid while aspartic acid (492.12 mg/kg) was the highest non-essential amino acid present in Pleurotus ostreatus. It had a higher concentration of acidic amino acids, 492.12 mg/100 g (77.87%), followed by neutral amino acids, 106.66 mg/100 g (16.88%) and least were the basic amino acids, 23.18 mg/100 g (3.67%). Based on the nutritional assessment of the Pleurotus ostreatus analyzed in this study, it can be concluded that it can serve as an important functional food source that can be exploited to meet the increasing food demands and reduce micronutrient deficiencies in many parts of the world, especially developing countries.

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Water Content of Plant Tissues: So Simple That Almost Forgotten?

Cited by 28 publications

References 182 publications

Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Variability in plant cell water dynamics as a genetic discriminatory method using terahertz quantum cascade laser-based laser feedback interferometry

Incorporation of Three Different Optical Trains into the IR-MALDESI Mass Spectrometry Imaging Platform to Characterize Artemisia annua

Assessing the nutritional quality of Pleurotus ostreatus (oyster mushroom)

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