Water Content of Lenses of Different Species

Hockwin, O.; Rast, F.; Rink, H.; Münninghoff, J.; Twenhöven, H.

doi:10.1159/000401962

Cited by 21 publications

(15 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is also in agreement with previous studies [21][22][23][24], An age-related continuous dehydration of the lens as observed in animal studies like mouse [25], rat, rabbit, dog and bovine lenses [19,24.26] does not take place in human lenses. With more sophisticated methods it becomes possible to differentiate between free and bound lens water, and to show alterations in these two water fractions [27,28].…”

Section: Discussionsupporting

confidence: 93%

Basic Biochemical Parameters of One Hundred Cataractous Lenses from Egyptian Patients

Rink

Ellayeh²,

Bours³

et al. 1995

Ophthalmic Res

Self Cite

View full text Add to dashboard Cite

Cataractous lenses from an extended population of 100 Egyptian patients whose age, sex and case history had been recorded, were collected and classified morphologically. After determination of the lens wet weights, lenses were microsectioned and basic parameters such as wet weight, dry weight, water content and contents of water-soluble and water-insoluble crystallins of the samples were determined. The data obtained document moderate as well as drastic changes of the investigated parameters along with aging and cataractogenesis, and extend our knowledge from small numbers of hitherto investigated human lenses to a considerably high number of cases.

show abstract

Section: Discussionsupporting

confidence: 93%

Basic Biochemical Parameters of One Hundred Cataractous Lenses from Egyptian Patients

Rink

Ellayeh²,

Bours³

et al. 1995

Ophthalmic Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…No differ ence in water content between both regions was found in the study by Nordmann [5]. This led to the view that in contrast to other mammalian lenses the water content is ho mogeneously distributed in humans [3,6]. However, using data originally reported by van Heyningen [4] and Nordmann [5], Bours et al [7] calculated that the human lens nucleus had a lower water content than the cortex.…”

Section: Introductionmentioning

confidence: 99%

“…The lens cortex of all mammals studied so far exhibits a higher water content than the respective lens nucleus [3]. In a study of postmortem human lenses, van Heyningen [4] concluded that the decrease in water con tent from the lens cortex to the nucleus is less steep in humans than in animals.…”

Section: Introductionmentioning

confidence: 99%

Regional Water Content of Clear and Cataractous Human Lenses

Deußen¹,

Pau

1989

Ophthalmic Res

View full text Add to dashboard Cite

The present study evaluates the regional water content of clear and cataractous human lenses. In order to determine the lens water, a freeze-drying technique was used which leaves behind only a very small amount of residual humidity. The effectiveness of freeze-drying was demonstrated in experiments with animal eye lenses (rat, pig, bovine). The results obtained revealed that, like in other mammalian lenses, the human lens cortex exhibits a significantly higher overall water content than the nucleus. This pattern was found in clear lenses as well as in lenses with early ‘senile’ cataracts. Subcapsulary cataracts present a unique feature as in these lenses the cortical water content is enhanced while the fresh lens weight is significantly reduced. Observations of postmortem human eye lenses indicate that regional differences in water content are greatly abolished 24–48 h after death

show abstract

“…Bovine nuclear lens tissue from 0 to 2 years has been shown to contain 56-60% water, while water content after 5 years has been shown to decrease to 54% [3,4], The tissues used in this study were from 0-to 1-year-old calves, so water compo sition was expected to be close to 60%. The mass percentages of water obtained in this study were 61.2 ± 1.6,61.2 ± 1.9, and 58.4 ± 1.9.…”

Section: Discussionmentioning

confidence: 98%

“…The protein concentration within the lens increases from the periphery toward the center in order to maintain a higher index of refraction in the nucleus. A newborn calf lens nucleus contains approximately 60% water, while the cortex has been reported as high as 78% water using lyophilization techniques [3]. The higher per centage of water in the cortex is due to the higher free water content of the tissue.…”

Section: Introductionmentioning

confidence: 99%

Determination of Water Content in Bovine Lenses Using Near-Infrared Spectroscopy

1997

View full text Add to dashboard Cite

Near-infrared spectral data of bovine nuclear lens tissue was collected in the 3,500- to 6,500-cm^––1 frequency range. The intensity ratios of the water peak at 5,194 cm^––1 to characteristic protein peaks at 4,862, 4,599, and 4,368 cm^––1 were determined for the nucleus of bovine lens samples. The water and protein mass percentages were determined by using a calibration curve established by spectra obtained from aqueous solutions with various concentrations of bovine serum albumin. The bovine fetal nucleus was found to contain 61.2 ± 1.6, 61.2 ± 1.9, and 58.4 ± 1.9 water using the ratio of the water peak intensity to each of the three characteristic protein peaks within each spectrum. These results are consistent with other analytical techniques, thus showing the accuracy of this technique for obtaining chemical information on lens tissue.

show abstract

Water Content of Lenses of Different Species

Cited by 21 publications

References 0 publications

Basic Biochemical Parameters of One Hundred Cataractous Lenses from Egyptian Patients

Basic Biochemical Parameters of One Hundred Cataractous Lenses from Egyptian Patients

Regional Water Content of Clear and Cataractous Human Lenses

Determination of Water Content in Bovine Lenses Using Near-Infrared Spectroscopy

Contact Info

Product

Resources

About