Water and temperature relations of Fusarium langsethiae strains and modelling of growth and T-2 and HT-2 mycotoxin production on oat-based matrices

Verheecke‐Vaessen, Carol; García-Cela, Esther; López-Prieto, Alejandro; Jonsdottir, Inga Osk; Medina, Ángel; Magan, Naresh

doi:10.1016/j.ijfoodmicro.2021.109203

Cited by 12 publications

(12 citation statements)

References 33 publications

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“…Furthermore, the production of DON and its derivatives was usually affected by environmental conditions such as temperature, humidity and host species [ 15 , 22 , 34 , 36 , 37 ]. In previous studies, the circumstantial effects from temperature and a w were demonstrated for potential contamination by inoculating F. graminearum , F. verticillioides , F. langsethiae , and F. meridionale in a cereal matrix such as maize, oat, or soybean [ 15 , 32 , 33 , 34 ]. However, studies on the effect of F. graminearum strains and abiotic factors on mycotoxin production in wheat-based matrixes are still not comprehensive.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, the environmental effects on fungal growth and potential mycotoxin contamination were demonstrated by inoculating F. graminearum , F. verticillioides , F. langsethiae , and F. meridionale in different cereal matrixes such as maize, oat, and soybean [ 15 , 32 , 33 , 34 ]. It has been reported that biosynthesis of DON and D3G is usually affected by environmental temperature, humidity, and hosts [ 22 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

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Exploration of Mycotoxin Accumulation and Transcriptomes of Different Wheat Cultivars during Fusarium graminearum Infection

Yan

et al. 2022

Toxins

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Fusarium graminearum is one of the most devastating diseases of wheat worldwide, and can cause Fusarium head blight (FHB). F. graminearum infection and mycotoxin production mainly present in wheat and can be influenced by environmental factors and wheat cultivars. The objectives of this study were to examine the effect of wheat cultivars and interacting conditions of temperature and water activity (aw) on mycotoxin production by two strains of F. graminearum and investigate the response mechanisms of different wheat cultivars to F. graminearum infection. In this regard, six cultivars of wheat spikes under field conditions and three cultivars of post-harvest wheat grains under three different temperature conditions combined with five water activity (aw) conditions were used for F. graminearum infection in our studies. Liquid chromatography tandem mass spectrometry (LC–MS/MS) analysis showed significant differences in the concentration of Fusarium mycotoxins deoxynivalenol (DON) and its derivative deoxynivalenol-3-glucoside (D3G) resulting from wheat cultivars and environmental factors. Transcriptome profiles of wheat infected with F. graminearum revealed the lower expression of disease defense-factor-related genes, such as mitogen-activated protein kinases (MAPK)-encoding genes and hypersensitivity response (HR)-related genes of infected Annong 0711 grains compared with infected Sumai 3 grains. These findings demonstrated the optimal temperature and air humidity resulting in mycotoxin accumulation, which will be beneficial in determining the conditions of the relative level of risk of contamination with FHB and mycotoxins. More importantly, our transcriptome profiling illustrated differences at the molecular level between wheat cultivars with different FHB resistances, which will lay the foundation for further research on mycotoxin biosynthesis of F. graminearum and regulatory mechanisms of wheat to F. graminearum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploration of Mycotoxin Accumulation and Transcriptomes of Different Wheat Cultivars during Fusarium graminearum Infection

Yan

et al. 2022

Toxins

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“…F. langsethiae Fe2391 strain was selected as this strain, originating from the UK, has been previously characterized as potent producer of T-2 and HT-2 toxin [ 6 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…More than 300 Fusarium species exist [ 4 ] with different ecophysiological responses to parameters such as temperature and water availability. F. langsethiae is a relatively slow colonizer of temperate cereals although the ability to produce mycotoxins may provide some competitiveness in the phyllosphere microbiome [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

De novo genome assembly and functional annotation for Fusarium langsethiae

et al. 2022

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Background Fusarium langsethiae is a T-2 and HT-2 mycotoxins producing species firstly characterised in 2004. It is commonly isolated from oats in Northern Europe. T-2 and HT-2 mycotoxins exhibit immunological and haemotological effects in animal health mainly through inhibition of protein, RNA and DNA synthesis. The development of a high-quality and comprehensively annotated assembly for this species is therefore essential in providing the molecular understanding and the mechanism of T-2 and HT-2 biosynthesis in F. langsethiae to help develop effective control strategies. Results The F. langsethiae assembly was produced using PacBio long reads, which were then assembled independently using Canu, SMARTdenovo and Flye. A total of 19,336 coding genes were identified using RNA-Seq informed ab-initio gene prediction. Finally, predicting genes were annotated using the basic local alignment search tool (BLAST) against the NCBI non-redundant (NR) genome database and protein hits were annotated using InterProScan. Genes with blast hits were functionally annotated with Gene Ontology. Conclusions We developed a high-quality genome assembly of a total length of 59 Mb and N50 of 3.51 Mb. Raw sequence reads and assembled genome is publicly available and can be downloaded from: GenBank under the accession JAFFKB000000000. All commands used to generate this assembly are accessible via GitHub: https://github.com/FadyMohareb/fusarium_langsethiae.

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“…For instance, in the study by Akbar et al (2016), under CC-related stress conditions, A. westerdijkiae, A. ochraceus, and A. steynii exhibited an increase in OTA production, whereas A. carbonarius and A. niger displayed a reduction in it. Moreover, some intraspecific differences in T-2 production by subjecting different strains of F. langsethiae to interacting CC-related abiotic factors were recently highlighted (Verheecke-Vaessen et al, 2021).…”

Section: Evolution and Adaptation Of Fungimentioning

confidence: 99%

Changing climate, shifting mycotoxins: A comprehensive review of climate change impact on mycotoxin contamination

Casu,

Camardo Leggieri,

Toscano

et al. 2024

Comp Rev Food Sci Food Safe

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Climate change (CC) is a complex phenomenon that has the potential to significantly alter marine, terrestrial, and freshwater ecosystems worldwide. Global warming of 2°C is expected to be exceeded during the 21st century, and the frequency of extreme weather events, including floods, storms, droughts, extreme temperatures, and wildfires, has intensified globally over recent decades, differently affecting areas of the world. How CC may impact multiple food safety hazards is increasingly evident, with mycotoxin contamination in particular gaining in prominence. Research focusing on CC effects on mycotoxin contamination in edible crops has developed considerably throughout the years. Therefore, we conducted a comprehensive literature search to collect available studies in the scientific literature published between 2000 and 2023. The selected papers highlighted how warmer temperatures are enabling the migration, introduction, and mounting abundance of thermophilic and thermotolerant fungal species, including those producing mycotoxins. Certain mycotoxigenic fungal species, such as Aspergillus flavus and Fusarium graminearum, are expected to readily acclimatize to new conditions and could become more aggressive pathogens. Furthermore, abiotic stress factors resulting from CC are expected to weaken the resistance of host crops, rendering them more vulnerable to fungal disease outbreaks. Changed interactions of mycotoxigenic fungi are likewise expected, with the effect of influencing the prevalence and co‐occurrence of mycotoxins in the future. Looking ahead, future research should focus on improving predictive modeling, expanding research into different pathosystems, and facilitating the application of effective strategies to mitigate the impact of CC.

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Water and temperature relations of Fusarium langsethiae strains and modelling of growth and T-2 and HT-2 mycotoxin production on oat-based matrices

Cited by 12 publications

References 33 publications

Exploration of Mycotoxin Accumulation and Transcriptomes of Different Wheat Cultivars during Fusarium graminearum Infection

Exploration of Mycotoxin Accumulation and Transcriptomes of Different Wheat Cultivars during Fusarium graminearum Infection

De novo genome assembly and functional annotation for Fusarium langsethiae

Changing climate, shifting mycotoxins: A comprehensive review of climate change impact on mycotoxin contamination

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