Watching cancer cells evolve through chromosomal instability

Johnson, Sarah C.; McClelland, Sarah E.

doi:10.1038/d41586-019-01709-2

Cited by 13 publications

(10 citation statements)

References 13 publications

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“…The PUMC-CRC1 cells displayed chromosomal instability providing rapid adaptation, which may be one factor leading to successful culture. Chromosomes of tumor cells in the early stage are mostly diploid, and chromosome instability increases with tumor progression and is often accompanied by changes in chromosome number and the generation of hybrid DNA 39 , 40 . Chromosomal deletion, cleavage and rearrangement lead to the activation of oncogenes or the inactivation of tumor suppressor genes, which promotes tumorigenesis and development 41 , 42 .…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Bian

Cao

Wang

et al. 2021

Sci Rep

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Colorectal cancer (CRC) is one of the most common and fatal gastrointestinal cancers worldwide. Considering their diversity, the establishment of new continuous CRC cell lines with clear genetic backgrounds will provide useful tools for exploring molecular mechanisms, screening and evaluating antitumor drugs in CRC studies. Our de novo CRC cell line, PUMC-CRC1 (Peking Union Medical College Colorectal Cancer 1) was derived from a 47-year-old Chinese female patient diagnosed with moderately to poorly differentiated colon adenocarcinoma. Multiple experiments were used for full characterization. The new cell line was epithelial-like and was passaged for more than 40 times, with a population doubling time of 44 h in vitro, detected by cell counts. The cells exhibited complicated chromosomal abnormalities. The tumor formation rate in SCID mice was 100%. The xenograft tumor was adenocarcinoma with poor to moderate differentiation by Haematoxylin and Eosin staining (H&E) sections. Immunohistochemistry (IHC) analysis and next-generation sequencing (NGS) revealed microsatellite stable (MSS), APC (p.T1493fs) inactivation, KRAS (p.G12V) activation, and SMAD4 (p.V506A) mutation. Quality control of the cell line proved mycoplasma negative and identical STR profile with that of the original tissue, and no interspecific or intraspecific cross contamination was detected. In conclusion, PUMC-CRC1 was a newly established and well characterized human colon cancer cell line, which might be a good model for both in vitro and in vivo studies of the mechanism of colon cancer progression and the treatment strategies for MSS CRC.

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Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Bian

Cao

Wang

et al. 2021

Sci Rep

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“…Chromosomal instability is mainly manifested by changes in chromosome structure and quantity. The chromosomes of early tumour cells are mostly diploid, and chromosome instability increases with tumour progression and is often accompanied by changes in chromosome number and the generation of hybrid DNA [24,25]. In gastric cancer, chromosomal instability is one of the main causes of genetic instability.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Establishment and characterization of a new gastric cancer cell line, XGC-1

Peng

Cai

2020

Cancer Cell Int

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Background: To establish a primary human gastric cancer cell line. Methods: Fresh gastric cancer tissue samples were separated into a cell suspension, and DMEM/F12 medium containing 10% foetal bovine serum was used for primary culture and subculture. The morphology of the cells was observed under a light microscope, and the cell growth curve was plotted. A soft agar colony formation assay was used to detect the colony formation ability of the cell line. Immunohistochemical methods were used to detect cytokeratin, vimentin and Ki-67, the chromosome G banding method was used to analyse the karyotype of the cells, and the tumourigenic ability of the cells was detected by subcutaneous inoculation of BALB/C nude mice. Results: We established a gastric cancer cell line from a 68-year-old male patient. This gastric cancer cell line was named XGC-1 and had a doubling time of approximately 48 h. The cell line displayed strong colony formation ability and tumourigenicity in BALB/C nude mice and had complicated chromosomal abnormalities. When nutrients were insufficient, the cells shed and floated in the medium, but adherent growth was observed in nutrient-rich conditions. Conclusions: The XGC-1 cell line will be useful for future studies of gastric cancer development, progression, metastasis and therapy.

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“…Chromosomes are constantly shuffling as they divide, and the genome has a high rate of change during this process. This is termed chromosomal instability (CIN) (Johnson and McClelland, 2019), and often leads to abnormal chromosome structure and aneuploidy. CIN is also thought to promote tumor evolution and metastasis (Bakhoum et al, 2018).…”

Section: Cell-in-cell and Changes In Related Cellular Characteristicsmentioning

confidence: 99%

Cell-in-Cell Phenomenon and Its Relationship With Tumor Microenvironment and Tumor Progression: A Review

Wang

et al. 2019

Front. Cell Dev. Biol.

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The term cell-in-cell, morphologically, refers to the presence of one cell within another. This phenomenon can occur in tumors but also among non-tumor cells. The cell-in-cell phenomenon was first observed 100 years ago, and it has since been found in a variety of tumor types. Recently, increasing attention has been paid to this phenomenon and the underlying mechanism has gradually been elucidated. There are three main related process: cannibalism, emperipolesis, and entosis. These processes are affected by many factors, including the tumor microenvironment, mitosis, and genetic factors. There is considerable evidence to suggest that the cell-in-cell phenomenon is associated with the prognosis of cancers, and it promotes tumor progression in most situations. Notably, in pancreatic cancer, the cell-in-cell phenomenon is associated with reduced metastasis, which is the opposite of what happens in other tumor types. Thus, it can also inhibit tumor progression. Studies show that cell-in-cell structure formation is affected by the tumor microenvironment, and that it may lead to changes in cellular characteristics. In this review, we summarize the different cell-in-cell processes and discuss their role in tumor progression and how they are regulated by different mechanisms.

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Watching cancer cells evolve through chromosomal instability

Cited by 13 publications

References 13 publications

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Establishment and characterization of a new human colon cancer cell line, PUMC-CRC1

Establishment and characterization of a new gastric cancer cell line, XGC-1

Cell-in-Cell Phenomenon and Its Relationship With Tumor Microenvironment and Tumor Progression: A Review

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