Wastewater-borne microalga Chlamydomonas sp.: A robust chassis for efficient biomass and biomethane production applying low-N cultivation strategy

Классен, Виктор; Blifernez-Klassen, Olga; Bax, Jördis; Kruse, Olaf

doi:10.1016/j.biortech.2020.123825

Cited by 25 publications

(11 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, our technology is also likely to be transferrable since a related Chlamydomonas sp. has shown good growth on wastewater ( 44 ) and PSR1 homologs carrying out P-starvation responses have been found in diverse taxa, such as the marine diatoms and higher plants ( 27, 45 ). Although we found hyper-accumulation to be transient, semi-/continuous cultures could be employed to hold the culture at peak biomass P. Alternatively, the moderate over-expresser lines also showed slower remobilization.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Slocombe

Zúñiga-Burgos

LiLi

et al. 2022

Preprint

View full text Add to dashboard Cite

Remediation using micro-algae offers an attractive solution to environmental phosphate (Pi) pollution. However, for maximum efficiency, pre-conditioning of algae to induce luxury phosphorus (P) uptake is needed. Here we show that natural pre-conditioning can be mimicked through over-expression of a single gene, the global regulator PSR1 (Myb transcription factor: Phosphate Starvation Response 1), raising P levels to 8% dry cell weight from 2% in control. Complete removal of Pi occurred in log phase, unlike the control. This was associated with increases in PolyP granule size and uptake of Mg2+, the principal counterion. Hyper-accumulation of P depended on a feed-forward mechanism, where a small set of Class I genes were activated despite abundant external Pi levels. This drove a reduction in external Pi levels, permitting more genes to be expressed (Class II), leading to more Pi -uptake. These discoveries enable a bio-circular approach of recycling nutrients from wastewater back to agriculture.

show abstract

Section: Discussionmentioning

confidence: 99%

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Slocombe

Zúñiga-Burgos

LiLi

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Mixed cultures have also been shown to promote growth of other Chlamydomonas spp. (Klassen et al, 2020). Here, the heavily domesticated C. reinhardtii UVM4 was robust enough to be the dominant microorganism in the AnMBR effluent under non-sterile conditions (Suppl.…”

Section: Resultsmentioning

confidence: 99%

Biomass generation and heterologous isoprenoid milking from engineered microalgae grown in anaerobic membrane bioreactor effluent

Freitas

Overmans

Medina

et al. 2022

Preprint

View full text Add to dashboard Cite

Wastewater (WW) treatment in anaerobic membrane bioreactors (AnMBR) is considered more sustainable than in their aerobic counterparts. However, outputs from AnMBR are mixed methane and carbon dioxide gas streams as well as ammonium- (N) and phosphate- (P) containing waters. Using AnMBR outputs as inputs for photoautotrophic algal cultivation can strip the CO2 and remove N and P from effluent which feed algal biomass generation. Recent advances in algal engineering have generated strains for concomitant high-value side product generation in addition to biomass, although only shown in heavily domesticated, lab-adapted strains. Here, investigated whether such a strain of Chlamydomonas reinhardtii could be grown directly in AnMBR effluent with CO2 at concentrations found in its off-gas. The domesticated strain was found to proliferate over bacteria in the non-sterile effluent, consume N and P to levels that meet general discharge or reuse limits, and tolerate cultivation in modelled (extreme) outdoor environmental conditions prevalent along the central Red Sea coast. High-value co-product milking was then demonstrated, up to 837 micro g / L culture in 96 h, in addition to algal biomass production, ~2.4 g CDW / L in 96 h, directly in effluents. This is the first demonstration of a combined bio-process that employs a heavily engineered algal strain to enhance the product generation potentials from AnMBR effluent treatment. This study shows it is possible to convert waste into value through use of engineered algae while also improve wastewater treatment economics through co-product generation.

show abstract

“…Also, Klassen et al. (2020) used nitrogen limited wastewater grown microalgae biomass for the production of good quality biogas. The obtained result showed that the continuous long‐term anaerobic digestion was produced specific biogas (765 ± 20 ml Ng/VS/d) and biomethane productivity (478 ± 15 ml Ng/VS/d).…”

Section: Bioenergy Generation From Microalgae‐based Wastewater Biorem...mentioning

confidence: 99%

Microalgal‐based remediation of wastewater: A step towards environment protection and management

Goswami

Agrawal

Verma

2022

Environmental Quality Mgmt

View full text Add to dashboard Cite

The widespread problem of water pollution is jeopardizing the ecosystem and our health. The contamination of freshwater through anthropogenic activities and its discharge into the ecosystems leads to the generation of wastewater. Wastewater mainly contains nutrient loads and the recent emerging contaminants such as pharmaceutical wastes, drugs, micropollutants, heavy metals, pesticides, phenolic compounds, and dyes have increased and contaminated the aquatic bodies. The existence of these emerging pollutants can cause serious threats to ecosystems and humans. Thus, conventional wastewater treatment was focused on the removal of solid compounds or nutrient loads. However, it could not remove the emerging contaminants and later microalgae that are photosynthetic eukaryotic microorganisms were found to be an efficient and eco‐friendly source for the remediation of diverse kinds of wastewater. Apart from nutrient removal, the algal biomass acts as the efficient feedstock for the generation of bioenergy products. However, limited data have been published regarding the remediation of emerging pollutants through microalgae. Thus, focusing on the recent gaps, the current review provides an overview of the potential of microalgae for the remediation of nutrient loads. Critically deliberate the recent advances and detailed summary of microalgae potential for the remediation of wastewaters containing high nutrient loads especially focused on the remediation of different emerging contaminants. It highlights the advanced approaches used in the microalgae remediation process and provides comprehensive information about the different bioenergy products such as liquid, gaseous, and bioelectricity generated through the utilization of wastewater‐grown microalgae.

show abstract

Wastewater-borne microalga Chlamydomonas sp.: A robust chassis for efficient biomass and biomethane production applying low-N cultivation strategy

Cited by 25 publications

References 44 publications

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Overexpression of PSR1 inChlamydomonas reinhardtiiinduces luxury phosphorus uptake

Biomass generation and heterologous isoprenoid milking from engineered microalgae grown in anaerobic membrane bioreactor effluent

Microalgal‐based remediation of wastewater: A step towards environment protection and management

Contact Info

Product

Resources

About