Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters

Kolarević, Stoimir; Micsinai, Adrienn; Szántó-Egész, Réka; Lukács, Alena; Kračun-Kolarević, Margareta; Djordjević, Ana; Milutinović, Danijela Vojnović; Marić, Jovana Jovanović; Kirschner, Alexander K. T.; Farnleitner, Andreas A H; Linke, Rita; Đukić, Anđelka; Kostić-Vuković, Jovana; Paunović, Momír

doi:10.1016/j.scitotenv.2022.156964

Cited by 4 publications

(3 citation statements)

References 38 publications

(60 reference statements)

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“…Surface waters heavily contaminated by sewage may also exhibit an epidemiological indicator function in terms of wastewater surveillance (e.g. (Kolarević et al 2022 , Maidana-Kulesza et al 2022 ).…”

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

“…applied an advanced sampling site characterization approach including measurement of human- (BacHum), ruminant- (BacR), and pig- (Pig-2-Bac) associated genetic faecal markers. By using this approach, they could trace and identify sites with significant raw sewage influence from human populations, which may serve as sampling locations for wastewater surveillance where no obvious sewage outlets exist (Kolarević et al 2022 ).…”

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

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Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

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The impacts on faecal pollution analysis using nucleic acid-based methods, such as PCR and sequencing, in health-related water quality research were assessed by rigorous literature analysis. A wide range of application areas and study designs has been identified since the first application more than 30 years ago (>1,100 publications). Given the consistency of methods and assessment types, we suggest defining this emerging part of science as a new discipline: genetic faecal pollution diagnostics (GFPD) in health-related microbial water quality analysis. Undoubtedly, GFPD has already revolutionised faecal pollution detection and microbial source tracking, the current core applications. GFPD is also expanding to many other research areas, including infection and health risk assessment, evaluation of microbial water treatment, and support of wastewater surveillance. In addition, storage of DNA extracts allows for biobanking, which opens up new perspectives. The tools of GFPD can be combined with cultivation-based standardised faecal indicator enumeration, pathogen detection, and various environmental data types, in an integrated data analysis approach. This comprehensive meta-analysis provides the scientific status quo of this field, including trend analyses and literature statistics, outlining identified application areas, and discussing the benefits and challenges of nucleic acid-based analysis in GFPD.

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Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

“…Wastewater-based epidemiology (WBE) has been used as a method of early detection and direct mitigation of poliovirus outbreaks in Israel and Egypt (Blomqvist et al 2012 ; Brouwer et al 2018 ; Kopel et al 2014 ) or Norovirus and Hepatitis A in Sweden (Berchenko et al 2017 ; Duintjer Tebbens et al 2017 ; Hellmér et al 2014 ). Currently, RT-qPCR assays are used for clinical trials and the detection of viral RNA in WWT (Acosta et al 2022 ; Kolarević et al 2022 ; Tanimoto et al 2022 ; Yanaç et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain

Trigo-Tasende

Vallejo

Rumbo‐Feal

et al. 2023

Environ Sci Pollut Res

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Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at the community level. Here we describe COVIDBENS, a wastewater surveillance program running from June 2020 to March 2022 in the wastewater treatment plant of Bens in A Coruña (Spain). The main goal of this work was to provide an effective early warning tool based in wastewater epidemiology to help in decision-making at both the social and public health levels. RT-qPCR procedures and Illumina sequencing were used to weekly monitor the viral load and to detect SARS-CoV-2 mutations in wastewater, respectively. In addition, own statistical models were applied to estimate the real number of infected people and the frequency of each emerging variant circulating in the community, which considerable improved the surveillance strategy. Our analysis detected 6 viral load waves in A Coruña with concentrations between 103 and 106 SARS-CoV-2 RNA copies/L. Our system was able to anticipate community outbreaks during the pandemic with 8–36 days in advance with respect to clinical reports and, to detect the emergence of new SARS-CoV-2 variants in A Coruña such as Alpha (B.1.1.7), Delta (B.1.617.2), and Omicron (B.1.1.529 and BA.2) in wastewater with 42, 30, and 27 days, respectively, before the health system did. Data generated here helped local authorities and health managers to give a faster and more efficient response to the pandemic situation, and also allowed important industrial companies to adapt their production to each situation. The wastewater-based epidemiology program developed in our metropolitan area of A Coruña (Spain) during the SARS-CoV-2 pandemic served as a powerful early warning system combining statistical models with mutations and viral load monitoring in wastewater over time.

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It is time to acknowledge coronavirus transmission via frozen and chilled foods: Undeniable evidence from China and lessons for the world

Han

Tang

Sun

et al. 2023

Science of The Total Environment

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Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters

Cited by 4 publications

References 38 publications

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain

It is time to acknowledge coronavirus transmission via frozen and chilled foods: Undeniable evidence from China and lessons for the world

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