Waste Water from Citrus Processing as a Source of Hesperidin by Concentration on Styrene−Divinylbenzene Resin

Abstract

This paper describes a procedure for recovering hesperidin from the waste water of orange juice processing, namely, yellow water, by concentration of diluted extracts on styrene-divinylbenzene resin. Turbid raw material flowing out from centrifuges of essential oil separation contains considerable amount of hesperidin ( approximately 1 g/L) mainly associated with solid particles. Yellow water was treated with calcium hydroxide until pH 12 to solubilize hesperidin, filtered, neutralized at pH 6, and loaded on resin up to saturation. Desorption with 10% ethanol aqueous solutions at different NaOH concentrations (0.23-0.92 M) assured high concentration of hesperidin in selected fractions (10-78 g/L), from which it precipitated in high yield and purity immediately after acidification at pH 5. Best results were obtained using 0.46 M NaOH as eluent: 71.5% of the adsorbed hesperidin was desorbed in 300 mL, with an overall 64% yield of isolated product at 95.4% purity (HPLC). These experiments can constitute a useful starting point for an industrial application.

Recovery, concentration and purification of phenolic compounds by adsorption: A review

“…Column adsorption of model solutions has also been assayed using resins (Ferreira and Rodrigues, 1995;Abburi, 2003;Otero et al, 2005a, b;Zabkova et al, 2006;Pan et al, 2008;Liu et al, 2010a,b), synthetic polymers (Romo et al, 2008), low cost adsorbents (Banat and Al-Asheh, 2001a;Rengaraj et al, 2002b;Navia et al, 2006), naturally occurring biopolymers (Nadavala et al, 2009), and biomass (Kumar et al, 2009a,b). Phenolic compounds from food products or byproducts can be selectively separated using low cost lignocellulosics (Sakanaka, 2003), resins (Grohmann et al, 1999;Di Mauro et al, 2000;Broadhurst and Rein, 2003;Chautard and Mayrand, 2003;Schieber et al, 2003;Manthey, 2004;Berardini et al, 2005;Fu et al, 2005;Achaerandio et al, 2007;Scordino et al, 2007;Díaz-Reinoso et al, 2010;Lu et al, 2010) and clay (Lopes et al, 2007). Continuous operation can be simulated using two parallel columns alternating adsorption and desorption-washing modes (di Mauro et al, 1999), eventually with a third security column (Camporro et al, 1994).…”

“…Oxidative polymerization of phenolics onto AC may result in low bioregeneration (de Jonge et al, 1996b). Aktas ß and Çeçen (2007) E, phenolics from apple juice M, phenolics from apple juice (kammerer et al, 2007) E, anthocyanins from molasses wastewater (Wang et al, 2008 b) M, anthocyanins from orange juice (Scordino et al, 2005) M, hydroxycinnamates from orange juice (Scordino et al, 2005) E, anthocyanins from orange juice (Scordino et al, 2005) E, hydroxycinnamates from orange juice (Scordino et al, 2005) NaOH + 10% E hesperidin from orange juice waste water (di Mauro et al, 2000) E, phenolics from barley husks autohydrolysates (Conde et al, 2008) E, phenolics from apple juice M, phenolics from apple juice (kammerer et al, 2007) E, anthocyanins from molasses wastewater (Wang et al, 2008 b) M, anthocyanins from orange juice (Scordino et al, 2005) M, hydroxycinnamates from orange juice (Scordino et al, 2005) E, anthocyanins from orange juice (Scordino et al, 2005) E, hydroxycinnamates from orange juice (Scordino et al, 2005) NaOH + 10% E hesperidin from orange juice waste water (di Mauro et al, 2000) E, phenolics from barley husks autohydrolysates (Conde et al, 2008) E, phenolicsfromapplejuice(Kammereret al mathematical modeling, mechanisms and influential factors on bioregeneration of organic and mineral adsorbents. Some of them, as rice husks, were easier to bioregenerate than ACs (Lee and Lim, 2003).…”

Interaction of apple polyphenols in a multi-compound system upon adsorption onto a food-grade resin

“…The use of resins for food production purposes is regulated by the US Food and Drug Administration and the Council of Europe, and foodgrade resins are now marketed for numerous applications. Adsorption processes are often developed empirically and successfully applied for large-scale purification and concentration of valuable compounds, however, the mechanisms behind the adsorption of components from complex systems still remain largely unknown (Di Mauro et al, 1999, 2000, 2002. This is due to the fact, that the adsorption behavior of each individual compound in such multi-compound systems needs to be taken into consideration as well as the characteristics of the solution, such as temperature, type of solvent, pH value and further substrate-specific components, and the physico-chemical properties of the adsorbent, such as type of resin matrix, porosity, surface area and pore radius.…”

Recovery of narirutin by adsorption on a non-ionic polar resin from a water-extract of Citrus unshiu peels

“…Adsorption has been previously employed in the removal of bitter substances, including limonin and naringin, during the processing of citrus juices (Hernandez, Couture, Rouseff, Chen, & Barros, 1992;Johnson & Chandler, 1988) and also to recover hesperidin from citrus peels or waste water during citrus processing (Manthey & Grohmann, 1996;Mauro, Fallico, Passerini, Rapisarda, & Maccarone, 1999;Mauro, Fallico, Passerini, & Maccarone, 2000).…”